1 research outputs found
Passive Sampling and High Resolution Mass Spectrometry for Chemical Profiling of French Coastal Areas with a Focus on Marine Biotoxins
Passive
samplers (solid phase adsorption toxin tracking: SPATT)
are able to accumulate biotoxins produced by microalgae directly from
seawater, thus providing useful information for monitoring of the
marine environment. SPATTs containing 0.3, 3, and 10 g of resin were
deployed at four different coastal areas in France and analyzed using
liquid chromatography coupled to high resolution mass spectrometry.
Quantitative targeted screening provided insights into toxin profiles
and showed that toxin concentrations and profiles in SPATTs were dependent
on the amount of resin used. Between the three amounts of resin tested,
SPATTs containing 3 g of resin appeared to be the best compromise,
which is consistent with the use of 3 g of resin in SPATTs by previous
studies. MassHunter and Mass Profiler Professional softwares were
used for data reprocessing and statistical analyses. A differential
profiling approach was developed to investigate and compare the overall
chemical diversity of dissolved substances in different coastal water
bodies. Principal component analysis (PCA) allowed for spatial differentiation
between areas. Similarly, SPATTs retrieved from the same location
at early, medium, and late deployment periods were also differentiated
by PCA, reflecting seasonal variations in chemical profiles and in
the microalgal community. This study used an untargeted metabolomic
approach for spatial and temporal differentiation of marine environmental
chemical profiles using SPATTs, and we propose this approach as a
step forward in the discovery of chemical markers of short- or long-term
changes in the microbial community structure