A new approach to modelling the shelf life of Gilthead seabream (Sparus aurata)

A total of 217 Gilthead seabreams were subdivided in four groups, according to four different storage conditions. All fish were evaluated by both Quality Index Method (QIM) and microbiological analysis, sampling skin, gills and flesh, separately. A QIM score predictive system was set by modelling the growth of microflora of skin, gills and flesh and coupling these predictions to each related partial QIM score (QIMSkin, QIMGills, QIMFlesh). The expression of QIM score as a function of bacterial behaviour was carried out by the employment of two coefficients. The predicted mean bacterial concentrations corresponding to the QIM score at 14 days were always near to Log 8 CFU g^{ -1} in the case of 'S' (skin) and 'G' (gills) series. Moreover, predicted QIM scores were in a good agreement with observed data, reproducing the observed mean time of rejection as well as the bacterial spoilage level (Log 8 CFU g^{ -1}), for all kinds of storage condition

Modelling Bacterial Dynamics in Food Products: Role of Environmental Noise and Interspecific Competition

In this paper we review some results obtained within the context of the predictive microbiology, which is a specific field of the population dynamics. In particular we discuss three models, which exploit tools of statistical mechanics, for bacterial dynamics in food of animal origin. In the first model, the random fluctuating behaviour, experimentally meas- ured, of the temperature is considered. In the second model stochastic differential equations are introduced to take into account the influence of physical and chemical variables, such as temperature, pH and activity water, subject to deter- ministic and random variations. The third model, which is an extended version of the second one, neglects the environ- mental fluctuations, and concentrates on the role of the interspecific bacterial interactions. The comparison between expected results and observed data indicates that the presence of noise sources and interspecific bacterial interactions improves the predictive features of the models analyzed

EFFECT OF SIDEROPHORES AGAINST E. COLI O157:H7

In this study, the inhibiting activity of siderophores produced by Pseudomonas fluorescens against Escherichia coli O157:7 was assayed. Siderophores were extracted from P. fluorescens cultures and preliminarily tested by C.A.S. reagent and UV. The siderophores activity against E. coli O157:H7 was characterised by the determination of Minimal Inhibiting Concentration (MIC). Furthermore, E. coli O157:H7 growth tests with and without siderophores were prepared at 12°C, pH 7 and 5, aw 0.997 and 0,988. The MIC was assessed at 7 μg/ml whereas the growth test showed a high inhibiting activity with the exception of tests at pH 5

BEHAVIOUR OF Aeromonas hydrophila IN SALTED SWORDFISH SAMPLES

A challenge test for Aeromonas hydrophila in salted swordfish samples was carried out. Particularly, 24 samples (250g) were experimentally contaminated, salted and stored at two different temperature regimes (fluctuating – F group - and non fluctuating – NF group – regime). The count of A. hydrophila, Enterobacteria and Lactic acid bacteria (LAB) as well as the determination of pH and aw were performed at 0, 19 43, 163, 187, 230, 320 and 368 hours whereas the temperature was monitored continuously by using 6 data-loggers. In both group, the mean concentrations of A. hydrophila did not exceed Log 3 cfu/g and decreased below the mean value of Log 1 cfu/g after 368 hours. However in the F group the A. hydrophila growth was slower and the decrease appeared slightly higher than NF group and this suggests the temperature fluctuations induces a more pronounced behaviour variability of A. hydrophila under stressing conditions

Development of a predictive model for the shelf-life of Atlantic mackerel (Scomber scombrus)

Despite its commercial value, the shelf-life of the Atlantic mackerel (Scomber scombrus) during refrigerated storage was poorly investigated. In this regard, the Quality Index Method (QIM) was proposed as a suitable scoring system for freshness and quality sensorial estimation of fishery products. This study aims to develop a deterministic mathematical model based on dynamic temperatures conditions and a suc-cessive statistical analysis of the results obtained. This model will be exploited to predict the shelf-life of the Atlantic mackerel based on specific storage temperatures. A total of 60 fresh fishes were subdivided into two groups and respectively stored in ice for 12 days at a constant temperature of 1±0.5°C (Group A) and a fluctuating temperature ranging between 1 and 7°C (Group B). Microbiological analysis and sensory evaluation through the QIM were performed on each fish at regular time inter-vals. A critical value of 6 Log cfu/g of spoilage bacteria (mainly psychotropic) associated with a significant decay of the sensorial characteristics was exceeded after 9 days of storage for Group A and 3 days for Group B. A reliable prediction of fish freshness was obtained by modelling the QIM as a function of the spoilage bacteria behaviour. A coefficient β of correlation was determined to convert the spoilage bacteria load into a Quality Index score. The adoption of mathematical predictive models to assess microbial behaviour under different environmental conditions is an interest-ing tool for food industries to maximize production and reduce waste

A new approach to predict the fish fillet shelf-life in presence of natural preservative agents

Three data sets concerning the behaviour of spoilage flora of fillets treated with natural preservative substances (NPS) were used to construct a new kind of mathematical predictive model. This model, unlike other ones, allows expressing the antibacterial effect of the NPS separately from the prediction of the growth rate. This approach, based on the introduction of a parameter into the predictive primary model, produced a good fitting of observed data and allowed characterising quantitatively the increase of shelf-life of fillets

Lagrangian for the Majorana-Ahluwalia Construct

The equations describing self/anti-self charge conjugate states, recently proposed by Ahluwalia, are re-written to covariant form. The corresponding Lagrangian for the neutral particle theory is proposed. From a group-theoretical viewpoint the construct is an example of the Nigam-Foldy-Bargmann-Wightman-Wigner-type quantum field theory based on the doubled representations of the extended Lorentz group. Relations with the Sachs-Schwebel and Ziino-Barut concepts of relativistic quantum theory are discussed.Comment: 10pp., REVTeX 3.0 fil