Results of four years of digital urban monitoring of Rattus norvegicus with RatMap in Hamburg including data on infestation near the surface and in underground sewers

Plenge-Bönig, A., Zickert, A., Baumgardt, K., Sammann, A

Long-term effect of xylitol chewing gum on dental caries

– About 85% ( n = 269) of the subjects who participated in the Ylivieska follow-up studies on the effect of xylitol chewing gum on dental caries during 1982–84 or 1982–85 were re-examined in 1987 for the analysis of possible long-term preventive effects. Further caries reduction was found 2 or 3 yr after the discontinuation of the use of xylitol. The effect was especially marked in girls; the reduction in caries increment in the post-use years was 60% for the 2-yr users, suggesting that more pronounced caries reduction was associated with the most regular use of xylitol. In teeth erupting during the first year of the use of xylitol gum the long-term preventive effect was greater than in other teeth. Several explanations are suggested: lasting effect of the microbiological changes in the mouth, bacterial colonization on newly erupted teeth by organisms other than S. mutatis , and/or thorough maturation of the teeth under favorable physico-chemical circumstances. The results suggest that the value of xylitol in caries prevention depends on the timing of the treatment in relation to the development of the dentitionPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75700/1/j.1600-0528.1989.tb00611.x.pd

Low-density granulocytes are related to shorter pregnancy duration but not to interferon alpha protein blood levels in systemic lupus erythematosus

BACKGROUND: An increased risk of pregnancy complications is seen in women with systemic lupus erythematosus (SLE), but the specific immunopathological drivers are still unclear. Hallmarks of SLE are granulocyte activation, type I interferon (IFN) overproduction, and autoantibodies. Here we examined whether low-density granulocytes (LDG) and granulocyte activation increase during pregnancy, and related the results to IFNα protein levels, autoantibody profile, and gestational age at birth. METHODS: Repeated blood samples were collected during pregnancy in trimesters one, two, and three from 69 women with SLE and 27 healthy pregnant women (HC). Nineteen of the SLE women were also sampled late postpartum. LDG proportions and granulocyte activation (CD62L shedding) were measured by flow cytometry. Plasma IFNα protein concentrations were quantified by single molecule array (Simoa) immune assay. Clinical data were obtained from medical records. RESULTS: Women with SLE had higher LDG proportions and increased IFNα protein levels compared to HC throughout pregnancy, but neither LDG fractions nor IFNα levels differed during pregnancy compared to postpartum in SLE. Granulocyte activation status was higher in SLE relative to HC pregnancies, and it was increased during pregnancy compared to after pregnancy in SLE. Higher LDG proportions in SLE were associated with antiphospholipid positivity but not to IFNα protein levels. Finally, higher LDG proportions in trimester three correlated independently with lower gestational age at birth in SLE. CONCLUSION: Our results suggest that SLE pregnancy results in increased peripheral granulocyte priming, and that higher LDG proportions late in pregnancy are related to shorter pregnancy duration but not to IFNα blood levels in SLE

A new method for chlorhexidine (CHX) determination: CHX release after application of differently concentrated CHX-containing preparations on artificial fissures

Aims of the study were (1) to establish a method for quantification of chlorhexidine (CHX) in small volumes and (2) to determine CHX release from differently concentrated CHX-containing preparations, varnishes, and a CHX gel applied on artificial fissures. CHX determination was conducted in a microplate reader using polystyrene wells. The reduced intensity of fluorescence of the microplates was used for CHX quantification. For verification of the technique, intra- and inter-assay coefficients of variation were calculated for graded series of CHX concentrations, and the lower limit of quantification (LLOQ) was determined. Additionally, artificial fissures were prepared in 50 bovine enamel samples, divided into five groups (A–E, n = 10) and stored in distilled water (7 days); A: CHX-varnish EC40; B: CHX-varnish Cervitec; C: CHX-gel Chlorhexamed; D: negative control, no CHX application; and E: CXH-diacetate standard (E1, n = 5) or CHX-digluconate (E2, n = 5) in the solution. The specimens were brushed daily, and CHX in the solution was measured. The method showed intra- and inter-assay coefficients of variation of <10 and <20%, respectively; LLOQ was 0.91–1.22 nmol/well. The cumulative CHX release (mean ± SD) during the 7 days was: EC40 (217.2 ± 41.8 nmol), CHX-gel (31.3 ± 8.5 nmol), Cervitec (18.6 ± 1.7 nmol). Groups A–C revealed a significantly higher CHX release than group D and a continuous CHX-release with the highest increase from day 0 to 7 for EC40 and the lowest for Chlorhexamed. The new method is a reliable tool to quantify CHX in small volumes. Both tested varnishes demonstrate prolonged and higher CHX release from artificial fissures than the CHX-gel tested

K-Decompositions and 3d Gauge Theories

This paper combines several new constructions in mathematics and physics. Mathematically, we study framed flat PGL(K,C)-connections on a large class of 3-manifolds M with boundary. We define a space L_K(M) of framed flat connections on the boundary of M that extend to M. Our goal is to understand an open part of L_K(M) as a Lagrangian in the symplectic space of framed flat connections on the boundary, and as a K_2-Lagrangian, meaning that the K_2-avatar of the symplectic form restricts to zero. We construct an open part of L_K(M) from data assigned to a hypersimplicial K-decomposition of an ideal triangulation of M, generalizing Thurston's gluing equations in 3d hyperbolic geometry, and combining them with the cluster coordinates for framed flat PGL(K)-connections on surfaces. Using a canonical map from the complex of configurations of decorated flags to the Bloch complex, we prove that any generic component of L_K(M) is K_2-isotropic if the boundary satisfies some topological constraints (Theorem 4.2). In some cases this implies that L_K(M) is K_2-Lagrangian. For general M, we extend a classic result of Neumann-Zagier on symplectic properties of PGL(2) gluing equations to reduce the K_2-Lagrangian property to a combinatorial claim. Physically, we use the symplectic properties of K-decompositions to construct 3d N=2 superconformal field theories T_K[M] corresponding (conjecturally) to the compactification of K M5-branes on M. This extends known constructions for K=2. Just as for K=2, the theories T_K[M] are described as IR fixed points of abelian Chern-Simons-matter theories. Changes of triangulation (2-3 moves) lead to abelian mirror symmetries that are all generated by the elementary duality between N_f=1 SQED and the XYZ model. In the large K limit, we find evidence that the degrees of freedom of T_K[M] grow cubically in K.Comment: 121 pages + 2 appendices, 80 figures; Version 2: reorganized mathematical perspective, swapped Sections 3 and

What is damaging the kidney in lupus nephritis?

Despite marked improvements in the survival of patients with severe lupus nephritis over the past 50 years, the rate of complete clinical remission after immune suppression therapy i