Effect of methyl jasmonate on production of 20- hydroxyecdysone and turkesterone in hairy roots of Silene linicola C.C.Gmelin

Methyl jasmonate (MeJ) affects the regulation of secondary metabolism, and it is considered to be a promising elicitor in the culture of cells, tissues and plant organs. High-performance liquid chromatography method was used to identify the composition of ecdysteroids in hairy roots of Silene linicola. MeJ was found to have a stimulating effect on ecdysteroid biosynthesis in this culture. Addition of MeJ at a concentration of 100 μM increased the biosynthesis of 20-hydroxyecdysone by 74% (day 3), and that of turkesterone by 35% (day 6). The share of turkesterone in total ecdysteroid content in the investigated samples was up to 60%, and the content of 20-hydroxyecdysone was up to 30%. The study shows that MeJ is a promising stimulator of ecdysteroid biosynthesis in hairy roots of S. linicola

Effect of methyl jasmonate on production of 20-hydroxyecdysone and turkesterone in hairy roots of Silene linicola C.C.Gmelin

Methyl jasmonate (MeJ) affects the regulation of secondary metabolism, and it is considered to be a promising elicitor in the culture of cells, tissues and plant organs. High-performance liquid chromatography method was used to identify the composition of ecdysteroids in hairy roots of Silene linicola. MeJ was found to have a stimulating effect on ecdysteroid biosynthesis in this culture. Addition of MeJ at a concentration of 100 μM increased the biosynthesis of 20-hydroxyecdysone by 74% (day 3), and that of turkesterone by 35% (day 6). The share of turkesterone in total ecdysteroid content in the investigated samples was up to 60%, and the content of 20-hydroxyecdysone was up to 30%. The study shows that MeJ is a promising stimulator of ecdysteroid biosynthesis in hairy roots of S. linicola

Morphological, histochemical and biochemical features of cultivated Rhodiola rosea (Altai Mountains ecotype)

The study analyzed the content and localization of phenolic compounds, in particular phenylpropanoids, of Rodiola rosea plants of Altai Mountains ecotype during the introduction period of 2–4 years in the conditions of the forest-steppe zone of Western Siberia. The plant material for the introduction experiment was obtained by in vitro method. HPLC was used to identify 11 phenolic compounds, including gallic acid, rosarin, rosavin, rosin, cinnamyl alcohol, rhodiosin, rhodionin, and kaempferol. The highest content of phenylpropenoids was found in rhizomes of the 4-year-old R. rosea plants: 1.02% rosarin, 2.64% rosavin, 1.05% rosin, 3.39% cinnamyl alcohol. Analysis of the phenylpropanoid profile showed that the predominant component in all the studied samples was cinnamyl alcohol (up to 58%). Histochemical studies identified phenolic substances in the rhizomes and roots of R. rosea, which are localized in parenchymal and vascular tissues. It was revealed that the total rhizome biomass exceeded that of the root, and by the 4th year of introduction, it was approximately 2-fold greater in dry weight. The study showed high biosynthetic potential and biological productivity of the studied R. rosea ecotype upon introduction

Chemical analysis of bioactive substances in seven siberian Saussurea species

Main groups of biologically active substances of seven siberian Saussurea species (S. controversa DC., S. latifolia Ledeb., S. parviflora (Poir.) DC., S. frolowii Ledeb, S. amara (L.) DC., S. salicifolia (L.) DC. and S. daurica Adams) have been studied using paper, thin-layer, performance liquid chromatography, IR spectroscopy, spectrophotometry and mass spectrometry with inductively coupled plasma. Siberian Saussurea species have a rich elemental composition and contain a variety of phenolic compounds, amino acids, polysaccharides. The majority of polysaccharides are accumulated by S. controversa, S. salicifolia and S. frolowii. These plants contain a significant amount of calcium that may be a species characteristic. All plants contain quercetin and its glycosides, in some species luteolin, kaempferol, glycosides of apigenin and myricetin were revealed. Phenolic acids with predominant content of caffeic, chlorogenic and cinnamic acids were found in all the species. The maximum amount of phenolic acids and flavonoids was determined in the grass of S. latifolia, S. controversa and S. daurica. Characteristic absorption bands of lactone carbonyl of sesquiterpenoids in IR spectrum found in S. latifolia, S. controversa, S. daurica, S. amara and S. salicifolia. HPLC / UV analysis showed that peaks with absorption maxima of 242-246 nm due to the presence of α,β-unsaturated ketone group in the structure of ecdysteroids were found in S. salicifolia, S. controversa, S. daurica and S. latifolia

Evaluation of the gastroprotective effect of the flavonoid complex from Lychnis chalcedonica L. on the models of experimental ulcerogenesis

Using rat and mouse models of neurogenic, ethanol-induced, and indometacin-induced damage to the gastric mucosa we demonstrated that course preventive treatment with favonoid complex from aerial parts of Lychnis chalcedonica L. increased the resistance of gastric mucosa to ulcerogenic factors of diferent etiology. The gastroprotective efect of the phytocomplex in a dose range of 16-1600 µg/kg was comparable with that of the reference drug plantaglucide and was superior to that of the reference drugs eleutherococcus extract and methyluracil in the therapeutic doses. The antiulcerogenic activity of Lychnis chalcedonica favonoid complex considerably exceeded activity of Lychnis chalcedonica L. extract demonstrated in our previous experiments