Axial plane optical microscopy.

We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues

Functional Evolution of BRCT Domains from Binding DNA to Protein

The BRCT domain (BRCA1 C-terminal domain) is an important signaling and protein targeting motif in the DNA damage response system. The BRCT domain, which mainly occurs as a singleton (single BRCT) or tandem pair (double BRCT), contains a phosphate-binding pocket that can bind the phosphate from either the DNA end or a phosphopeptide. In this work, we performed a database search, phylogeny reconstruction, and phosphate-binding pocket comparison to analyze the functional evolution of the BRCT domain. We identified new BRCT-containing proteins in bacteria and eukaryotes, and found that the number of BRCT-containing proteins per genome is correlated with genome complexity. Phylogeny analyses revealed that there are two groups of single BRCT domains (sGroup I and sGroup II) and double BRCT domains (dGroup I and dGroup II). These four BRCT groups differ in their phosphate-binding pockets. In eukaryotes, the evolution of the BRCT domain can be divided into three phases. In the first phase, the sGroup I BRCT domain with the phosphate-binding pocket that can bind the phosphate of nicked DNA invaded eukaryotic genome. In the second phase, the phosphate-binding pocket changed from a DNA-binding type to a protein-binding type in sGroup II. The tandem duplication of sGroup II BRCT domain gave birth to double BRCT domain, from which two structurally and functionally distinct groups were evolved. The third phase is after the divergence between animals and plants. Both sGroup I and sGroup II BRCT domains originating in this phase lost the phosphate-binding pocket and many evolved protein-binding sites. Many dGroup I members were evolved in this stage but few dGroup II members were observed. The results further suggested that the BRCT domain expansion and functional change in eukaryote may be driven by the evolution of the DNA damage response system

Multimedia over massive MIMO wireless systems

To satisfy the massive wireless traffic transmission generated by multimedia applications, the massive multi-input-multi-output (MIMO) wireless system has emerged as a possible solution for future 5G wireless communication systems. However, the mutual coupling effect of massive MIMO systems has a negative effect potential on the wireless capacity. In this paper, the receive diversity gain is first defined and analyzed for massive MIMO wireless systems. Furthermore, we propose an effective capacity with the mutual coupling effect and the quality of service (QoS) statistical exponent constraint for multimedia massive MIMO wireless systems. Based on numerical results, the effective capacity approaches the effective capacity with the antenna spacing of d = 0.5λ when the antenna spacing is increased in the massive MIMO antenna array

Influence of nonideal voltage measurement on parameter estimation in permanent-magnet synchronous machines

This paper investigates the influence of nonideal voltage measurements on the parameter estimation of permanentmagnet synchronous machines (PMSMs). The influence of nonideal voltage measurements, such as the dc bus voltage drop, zero shift in the amplifier, and voltage source inverter nonlinearities, on the estimation of different machine parameters is investigated by theoretical and experimental analysis. For analysis, a model-reference-adaptive-system-based estimator is first described for the parameter estimation of the q-axis inductance, stator winding resistance, and rotor flux linkage. The estimator is then applied to a prototype surface-mounted PMSM to investigate the influence of nonideal voltage measurement on the estimation of various machine parameter values. It shows that, at low speed, the inverter nonlinearity compensation has significant influence on both the rotor flux linkage and winding resistance estimations while, at high speed, it has significant influence only on the winding resistance estimation and has negligible influence on the rotor flux linkage estimation. In addition, the inverter nonlinearity compensation will not influence the q-axis inductance estimation when it is under id = 0 control. However, the dc bus voltage drop due to the load variation and zero shift in the amplifier will significantly influence the q-axis inductance estimation. © 2011 IEEE