21 research outputs found

    Research on the Reform of Teaching Management Emergency Mechanism in University Based on Big Data

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    Sudden major public health events once again test the ability of the whole society to deal with emergencies. Universities are no exception. There are many kinds of management work in Colleges and universities, among which teaching management is the most important one, which is also one of the most affected in this epidemic situation. Therefore, in view of the problems of teaching management in Colleges and universities exposed in the epidemic, combined with the characteristics of independent colleges, starting from the aspects of teaching guarantee mechanism, teaching supervision mechanism, teaching process construction, teaching resources construction and so on, the advantages of big data technology, such as large amount of information, easy to communicate and easy to integrate, are fully used to put forward a set of open-minded, perfect mechanism and advanced technology teaching management emergency response mechanism

    The Impact of Fertilizer Amendments on Soil Autotrophic Bacteria and Carbon Emissions in Maize Field on the Semiarid Loess Plateau

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    Soil autotrophic bacteria play a crucial role in regulating CO2 fixation and crop productivity. However, the information is limited to how fertilization amendments alter soil autotrophic bacterial community, crop yield, and carbon emission efficiency (CEE). Here, we estimated the impact of the structure and co-occurrence network of soil autotrophic bacterial community on maize yield and CEE. A long-term field experiment was conducted with five fertilization treatments in semiarid Loess Plateau, including no amendment (NA), chemical fertilizer (CF), chemical fertilizer plus commercial organic fertilizer (SC), commercial organic fertilizer (SM), and maize straw (MS). The results showed that fertilization amendments impacted the structure and network of soil Calvin–Benson–Bassham (CBB) (cbbL) gene-carrying bacterial community via changing soil pH and NO3–N. Compared with no amendment, the cbbL-carrying bacterial diversity was increased under the SC, SM, and MS treatments but decreased under the CF treatment. Soil autotrophic bacterial network contained distinct microbial modules that consisted of closely associated microbial species. We detected the higher abundances of soil cbbL-carrying bacterial genus Xanthobacter, Bradyrhizobium, and Nitrosospira. Structural equation modeling further suggested that the diversity, composition, and network of autotrophic bacterial community had strongly positive relationships with CEE and maize yield. Taken together, our results suggest that soil autotrophic bacterial community may drive crop productivity and CEE, and mitigate the atmospheric greenhouse effect

    A multimodal cell census and atlas of the mammalian primary motor cortex

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    ABSTRACT We report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex (MOp or M1) as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties, and cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance the collective knowledge and understanding of brain cell type organization: First, our study reveals a unified molecular genetic landscape of cortical cell types that congruently integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a unified taxonomy of transcriptomic types and their hierarchical organization that are conserved from mouse to marmoset and human. Third, cross-modal analysis provides compelling evidence for the epigenomic, transcriptomic, and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types and subtypes. Fourth, in situ single-cell transcriptomics provides a spatially-resolved cell type atlas of the motor cortex. Fifth, integrated transcriptomic, epigenomic and anatomical analyses reveal the correspondence between neural circuits and transcriptomic cell types. We further present an extensive genetic toolset for targeting and fate mapping glutamatergic projection neuron types toward linking their developmental trajectory to their circuit function. Together, our results establish a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties

    Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

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    For potato production, continuous cropping (CC) could lead to autotoxicity buildup and microflora imbalance in the field soil, which may result in failure of crops and reduction in yield. In this study, non-targeted metabolomics (via liquid chromatography with tandem mass spectrometry (LC–MS/MS)) combined with metagenomic profiling (via high-throughput amplicon sequencing) were used to evaluate correlations between metabolomics of potato root exudates and communities of bacteria and fungi around potato plants to illustrate the impacts of CC. Potato plants were grown in soil collected from fields with various CC years (0, 1, 4, and 7 years). Metabolomic analysis showed that the contents and types of potential autotoxins in potato root exudates increased significantly in CC4 and CC7 plants (i.e., grown in soils with 4 and 7 years of CC). The differentially expressed metabolites were mainly produced via alpha-linolenic acid metabolism in plant groups CC0 and CC1 (i.e., no CC or 1 year CC). The metabolomics of the groups CC4 and CC7 became dominated by styrene degradation, biosynthesis of siderophore group non-ribosomal peptides, phenylpropanoid biosynthesis, and biosynthesis of various plant secondary metabolites. Continuous cropping beyond 4 years significantly changed the bacterial and fungal communities in the soil around the potato crops, with significant reduction of beneficial bacteria and accumulation of harmful fungi. Correlations between DEMs and microflora biomarkers were established with strong significances. These results suggested that continuous cropping of potato crops changed their metabolism as reflected in the plant root exudates and drove rhizosphere microflora to directions less favorable to plant growth, and it needs to be well managed to assure potato yield.This article is published as Xing, Yanhong, Pingliang Zhang, Wenming Zhang, Chenxu Yu, and Zhuzhu Luo. "Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora." Frontiers in Microbiology 14: 1318586. doi: https://doi.org/10.3389/fmicb.2023.1318586. © 2024 Xing, Zhang, Zhang, Yu and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)

    Land Use Affects Soil Water Balance and Soil Desiccation within the Soil Profile: Evidence from the Western Loess Plateau Case

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    This study evaluated the properties of soil water dynamics and desiccation to a depth of 500 cm and tested the idea that land use affects soil drying in deep profiles. Four land use types were chosen: farmland, artificial forest and grassland, and abandoned land. Soil water content was most outstanding under long-term wheat fields, but average soil water content under artificial vegetation of Caragana korshinskii Kom. and alfalfa dropped to 6–8% within the 160–500 cm soil profile, very near to the 7.0% wilting point. Long-term continuous maize cultivation in a fully mulched ridge–furrow system significantly depleted soil water and resulted in a dried soil layer with a thickness of 240 cm. Short-term or long-term land abandonment enhanced soil reservoir and reduced soil water storage deficit degree compared to long-term maize and artificial vegetation. Soil water storage in the 160–500 cm soil profile was depleted by 240 and 464 mm under long-term maize and Caragana korshinskii Kom., respectively, by 267, 319, 381, and 463 mm under 5-, 10-, 20-, and 30-year alfalfa, and by −58, 278, 234, and 93 mm under 5-, 10-, 20-, and 30-year abandonment land, respectively, compared to long-term wheat. Based on the analysis of long-term experimental results, this study shows that the phenomenon of soil drying caused by long-term intensive maize production cannot be ignored in semi-arid areas and that natural re-vegetation under long-term abandonment, rather than artificial vegetation, may be the best type of vegetation reconstruction for this region based on soil water balances

    Continuous Monoculture of Alfalfa and Annual Crops Influence Soil Organic Matter and Microbial Communities in the Rainfed Loess Plateau of China

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    Cropping systems are structured to maximize crop yields and increase sustainability in agricultural production. A field study was conducted to investigate different long-term cropping systems on soil organic matter and microbial communities. The cropping systems studied were: (i) a 14-year continuous alfalfa (Medicago sativa L.) (CA), (ii) a 9-year alfalfa removed and rotated with 4–5 years continuous annual crops (spring wheat (Triticum aestivum L.), maize (Zea mays L.), potato (Solanum tuberosum L.), and millet (Panicum miliaceum L.)), and (iii) a 5-year field fallow after alfalfa. Results showed that continued annual crops decreased total organic C and labile organic C by 10 to 20% and 17 to 34% in the topsoil (0–30 cm), and by 15 to 35% and 20 to 46% in the subsoil (30–60 cm), respectively, compared with CA. Similar trends were found in soil total N concentration, which decreased by 7 to 20% in the topsoil. Highest microbial biomass C was found in CA. Shannon-Wiener diversity and substrate richness of soil microbes measured by Biolog EcoPlates was significantly affected by cropping system with CA exhibiting a higher degree of soil microbial functional diversity in the topsoil, while the lowest values were found in the alfalfa-potato system. The higher soil organic matter content and functional diversity of soil microbe in CA indicates that soil nutrition and microbial activity did not limit alfalfa development and growth in the dryland area. The lower microbial activity and functional diversity observed in the potato field indicates the importance of crop selection in cropping systems

    Land Use Affects Soil Water Balance and Soil Desiccation within the Soil Profile: Evidence from the Western Loess Plateau Case

    No full text
    This study evaluated the properties of soil water dynamics and desiccation to a depth of 500 cm and tested the idea that land use affects soil drying in deep profiles. Four land use types were chosen: farmland, artificial forest and grassland, and abandoned land. Soil water content was most outstanding under long-term wheat fields, but average soil water content under artificial vegetation of Caragana korshinskii Kom. and alfalfa dropped to 6–8% within the 160–500 cm soil profile, very near to the 7.0% wilting point. Long-term continuous maize cultivation in a fully mulched ridge–furrow system significantly depleted soil water and resulted in a dried soil layer with a thickness of 240 cm. Short-term or long-term land abandonment enhanced soil reservoir and reduced soil water storage deficit degree compared to long-term maize and artificial vegetation. Soil water storage in the 160–500 cm soil profile was depleted by 240 and 464 mm under long-term maize and Caragana korshinskii Kom., respectively, by 267, 319, 381, and 463 mm under 5-, 10-, 20-, and 30-year alfalfa, and by −58, 278, 234, and 93 mm under 5-, 10-, 20-, and 30-year abandonment land, respectively, compared to long-term wheat. Based on the analysis of long-term experimental results, this study shows that the phenomenon of soil drying caused by long-term intensive maize production cannot be ignored in semi-arid areas and that natural re-vegetation under long-term abandonment, rather than artificial vegetation, may be the best type of vegetation reconstruction for this region based on soil water balances

    Exploring Optimal Cropping System to Improve the Water Use Efficiency and Soil Water Restoration after Lucerne-to-Crop Conversion in the Semiarid Environment

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    Due to depleting water supplies and the cultivation of high water-demanding crops such as lucerne, the effect of water deficits in crop production has become a major concern, especially in semiarid regions of China. A six-year field experiment (2012–2018) was conducted to evaluate soil water recovery and soil fertility after lucerne-to-crop conversions on the western Loess Plateau of China. Six rotation treatments (lucerne initially grew from 2003 to 2012 followed by the rotation of other crops or fallow until assessments in 2018) were: (1) lucerne (Medicago sativa L.)–lucerne (L-L); (2) lucerne–fallow (L-F); (3) lucerne–wheat (Triticum aestivum L.) (L-W); (4) lucerne–corn (Zea mays L.) (L-C); (6) lucerne–potato (Solanum tuberosum L.) (L-P); and (6) lucerne–millet (Setaria italica) (L-M). The same crops were grown each year after cultivation during 2013–2018. According to the findings, all rotation types gradually increased the soil water content, with the 0–110 cm soil layer experiencing the maximum soil water replenishment rate, followed by the 110–200 and 200–300 cm soil layers. After converting lucerne to crops, the amount of organic carbon, total nitrogen, and mineral nitrogen in the soil decreased, whereas total phosphorus and accessible phosphorus increased. Soil bulk density was reduced under rotation treatments. Soil water absolute restoration index was the highest under L-F, followed by L-C, L-W, L-P, and L-M. The rate of soil water recovery was 39.5, 33.0, 33.7, 33.5, 29, and 8.2 mm yr−1 under L-F, L-W, L-C, L-P, L-M, and L-L, respectively. The net economic return was greatest under L-C, followed by L-L, L-W, L-P, and L-M. From the analysis of the long-term experimental results, this study shows that the effect of soil water restoration is greatest when continuous alfalfa is converted into wheat, corn, and potato or fallow after 9 years. When the economic benefits and soil moisture recovery are considered comprehensively, corn sown in a ridge–furrow system with fully plastic film annual mulching is the most suitable field management practice after lucerne-to-crop conversion on the western Loess Plateau of China

    Evolution of soil and water conservation in rain-fed areas of China

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    Rain-fed (dryland) farming is an ancient agricultural production system in China. It occurs widely across almost the whole country, especially in the Northwest and North China. The semi-arid Loess Plateau is the most important region of rain-fed farming in China, but unfortunately, soil erosion on the Loess Plateau area is the highest in China, and indeed amongst the highest in the world. This highlights the necessity for developing practices that can reduce soil and water erosion, improve soil water use efficiency, improve crop productivity, and reduce rural poverty in the region. Many techniques of soil and water conservation are being used in rain-fed areas of China, including such systems as mulch, ridge and furrow systems. The Appendix describes a unique system of soil and water conservation, called Shatian. Modern research on conservation tillage (No Till), although essential for reducing erosion, increasing crop productivity, and ameliorating poverty, is just beginning in China. Modern conservation tillage research started in the1990s' with support from Australia and other countries. The procedures, however, were modified to be in accord with local conditions and prevailing farmer experiences. With 10 years of experimentation, results show that the most successful conservation practice on the Western Loess Plateau is no till with stubble retention. This technique helps to conserve soil water, increases soil organic carbon, improves soil structure and water infiltration, reduces soil and water erosion, and improves crop productivity and sustainability of rain-fed farming systems. However, its adoption rate remains low due to barriers such as traditional attitude, insufficient rural extension, and so forth

    Irrigation as an Effective Way to Increase Potato Yields in Northern China: A Meta-Analysis

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    A meta-analysis was conducted with the aim of exploring the influence of irrigation on potato yield, evapotranspiration (ET), and water-use efficiency (WUE) in northern China, considering factors such as irrigation methods, growing region, irrigation water-use efficiency (IWUE), irrigation frequency, soil types, and nitrogen (N) fertilizer rate. Overall, irrigation significantly increased potato yield and ET by an average of 45 and 54% compared to non-irrigation, respectively, but did not significantly increase the WUE. The increase in potato yield under irrigation is the most evident in aeolian sandy soil in northeast China and northwest China. Drip irrigation demonstrated the highest positive impact on both yield and WUE. Optimal yields were achieved with an irrigation amount ranging from 100 to 200 mm, while the highest WUE was observed with an irrigation amount of 30–50 mm. When the amount of irrigation exceeded 100 mm, the irrigation significantly resulted in decreased WUE compared to non-irrigation. The relative increase in yield per unit of irrigation amount and IWUE gradually decreased and eventually stabilized when the irrigation amount exceeded 100 mm. Therefore, the yield and WUE perform best when the irrigation amount is below 100 mm and the irrigation frequency is less than three times for sprinkling and flood irrigation methods. The greatest increases in yield and WUE under irrigation were found under a moderate N rate (150–250 kg N ha−1). Additionally, the relative increase in yield per unit of irrigation amount decreases gradually as the organic matter content increases. These findings suggest that the optimal benefits from irrigation might be realized when the irrigation amount is below 100 mm, with a moderate nitrogen fertilizer application and an irrigation frequency of three times. However, it is essential to consider local environmental factors such as the growing region, soil types, and organic carbon content
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