History of the formation of high art of modern military medicine of Ukraine

В статье рассматриваются вопросы современной военной медицины в Украине как самостоятельного государства. Были составлены основные вехи национальной военной медицины, и были выделены особенности ее реформы. Также в статье предоставлен анализ мер регулирования и поддержки реформы военной медицины. Учитывая данные, полученные из большого количества архивных источников и административных материалов, особое внимание было уделено трансформации управления государственной медицинской поддержкой Вооруженных Сил Украины. Определены некоторые недостатки в реформировании военно-медицинского образования, показаны основные перспективные направления его развития

In vitro nuclear interactome of the HIV-1 Tat protein

<p>Abstract</p> <p>Background</p> <p>One facet of the complexity underlying the biology of HIV-1 resides not only in its limited number of viral proteins, but in the extensive repertoire of cellular proteins they interact with and their higher-order assembly. HIV-1 encodes the regulatory protein Tat (86–101aa), which is essential for HIV-1 replication and primarily orchestrates HIV-1 provirus transcriptional regulation. Previous studies have demonstrated that Tat function is highly dependent on specific interactions with a range of cellular proteins. However they can only partially account for the intricate molecular mechanisms underlying the dynamics of proviral gene expression. To obtain a comprehensive nuclear interaction map of Tat in T-cells, we have designed a proteomic strategy based on affinity chromatography coupled with mass spectrometry.</p> <p>Results</p> <p>Our approach resulted in the identification of a total of 183 candidates as Tat nuclear partners, 90% of which have not been previously characterised. Subsequently we applied <it>in silico </it>analysis, to validate and characterise our dataset which revealed that the Tat nuclear interactome exhibits unique signature(s). First, motif composition analysis highlighted that our dataset is enriched for domains mediating protein, RNA and DNA interactions, and helicase and ATPase activities. Secondly, functional classification and network reconstruction clearly depicted Tat as a polyvalent protein adaptor and positioned Tat at the nexus of a densely interconnected interaction network involved in a range of biological processes which included gene expression regulation, RNA biogenesis, chromatin structure, chromosome organisation, DNA replication and nuclear architecture.</p> <p>Conclusion</p> <p>We have completed the <it>in vitro </it>Tat nuclear interactome and have highlighted its modular network properties and particularly those involved in the coordination of gene expression by Tat. Ultimately, the highly specialised set of molecular interactions identified will provide a framework to further advance our understanding of the mechanisms of HIV-1 proviral gene silencing and activation.</p

Evaluation of the capabilities of the medical support system according to the requirements of NATO standards

В статье произведен анализ методики оценки возможностей подразделений медицинской службы Вооруженных Сил Украины. Цель исследования. Провести научный анализ мировых и отечественных источников информации по соответствию их содержания современным взглядам на организацию и проведение мероприятий оборонного планирования и оценивания возможностей медицинской службы в вооруженных силах ведущих стран мира. Методы исследования: описательный, библиосемантический, статистический, системного анализа, концептуального моделирования для анализа и синтеза данных в оборонном планировании на основе возможностей и их оценки в работе медицинской службы вооруженных сил. Научная новизна заключается в анализе и обосновании направления дальнейших исследований по совершенствованию методологии оценки возможностей медицинской службы Вооруженных Сил Украины. Выводы: оборонное планирование, базирующееся на основе их реальных возможностей, является современным этаном развития системы стратегического планирования. Определение рисков происходит проведением оценивания возможностей, однако, данный процесс требует дальнейшего развития и адаптации к новым конкретным обстоятельствам. The article analyzes the methodology for assessing the capabilities of the medical service units of the Armed Forces of Ukraine. According to the Strategic Defense Bulletin of Ukraine, the purpose of defense reform in Ukraine is the acquisition and maintenance by the defense forces of the required combat readiness level and ability to fulfill the tasks of defense of the state (defense capabilities), effective response to emerging military threats and military-political challenges to national security, the level of interoperability of the Armed Forces of Ukraine with units of the armed forces of NATO member states and the EU to fulfill common tasks in international peacekeeping and security operations. Purpose of the study. Conduct a scientific analysis of world and domestic sources of information on modem views on the organization and conduct of defense planning activities and assessing the capabilities of the medical service in the armed forces of the leading countries of the world. Research methods are descriptive, bibliosemantic, statistical, system analysis, conceptual modeling for the analysis and synthesis of defense planning data based on the capabilities and assessment of the capabilities of the armed forces medical service. The scientific novelty lies in the analysis and justification of the direction of further research to improve the methodology for assessing the capabilities of the medical service of the Armed Forces of Ukraine. Conclusions: Capability-based defense planning is the next stage in the development of the strategic planning system, which greatly improves the quality of planning, enhances the ability of the Armed Forces of Ukraine to perform its intended tasks, in particular, introduces a mechanism for identifying and correcting risks. The directions of improvement of the methodology for assessing the capabilities of the medical service of the Armed Forces of Ukraine are analyzed. The identification of risks occurs through an assessment of opportunities, but the process requires development and adaptation. The lack of effective approaches to capacity assessment, as well as the need for developing new or improving existing algorithms and assessment methods are highlighted