10 research outputs found
Gemcitabine Inhibits Poliovirus Replication and Interferes with Viral RNA Elongation
While we are approaching the end game of global eradication of circulating wild-type polioviruses(PV), vaccination with oral poliovirus vaccine (OPV) has led to emergence of circulating vaccine-derived poliovirus (cVDPV) and vaccine-associated paralytic poliomyelitis (VAPP). Complete cessation of all poliovirus infections may require stopping use of OPV and formulating improved vaccines and new antiviral drugs. Currently, no licensed drugs are available to treat chronically infected poliovirus excreters. Here, we created a modified PV expressing Gaussia Luciferase (PV-GLuc) and developed a cell-based high-throughput screening (HTS) antiviral assay. Using the validated HTS assay, we screened the FDA-approved drug library of compounds and identified candidate agents capable of inhibiting PV replication. We then characterized anti-poliovirus activity for the best hit, Gemcitabine, a nucleoside analogue used in tumor chemotherapy. We found that Gemcitabine inhibited PV Mahoney replication with IC50 of 0.3μM. It completely protected HeLa cells from PV-induced cytopathic effects at 5μM, without detectable toxicity for cell viability. Furthermore, Gemcitabine metabolite directly inhibited the ability of PV RNA polymerase to synthesize or elongate PV RNA. Since PV RNA polymerase is somehow conserved among species in Picornaviridae family, Gemcitabine may be further developed as an attractive broad-spectrum antiviral for PV and others
Data_Sheet_5_Vitamin D deficiency increases the risk of bacterial vaginosis during pregnancy: Evidence from a meta-analysis based on observational studies.PDF
BackgroundBacterial vaginosis (BV) is the most common microbiological syndrome in women of childbearing age, causing numerous adverse health issues in pregnant women. Several observational studies have discussed the association between vitamin D deficiency and the risk of BV during pregnancy, but the results were inconclusive. Therefore, this meta-analysis aimed to explore the association between vitamin D deficiency and BV risk in pregnant women.Materials and methodsWe searched four databases, including PubMed, Embase, Cochrane Library, and Web of Science, from their inception to July 2022. Pooled odds ratios (OR) with corresponding 95% confidence intervals (CI) were estimated using random effects models. Additionally, we conducted subgroup analyses to identify the potential sources of between-study heterogeneity. Sensitivity analysis was performed using the method of exclusion, one study at a time. Publication bias was examined using Egger’s test and funnel plot.ResultsA total of 14 studies from 13 articles including 4,793 participants were eligible for this meta-analysis. The outcome showed that vitamin D deficiency may increase the risk of BV during pregnancy by 54% (OR, 1.54; 95% CI, 1.25–1.91; P ConclusionThis meta-analysis showed that vitamin D deficiency is positively associated with the risk of BV during pregnancy. Further high-quality prospective cohort studies are needed to determine whether vitamin D intake reduces the prevalence of BV in pregnant women.</p
Data_Sheet_2_Vitamin D deficiency increases the risk of bacterial vaginosis during pregnancy: Evidence from a meta-analysis based on observational studies.PDF
BackgroundBacterial vaginosis (BV) is the most common microbiological syndrome in women of childbearing age, causing numerous adverse health issues in pregnant women. Several observational studies have discussed the association between vitamin D deficiency and the risk of BV during pregnancy, but the results were inconclusive. Therefore, this meta-analysis aimed to explore the association between vitamin D deficiency and BV risk in pregnant women.Materials and methodsWe searched four databases, including PubMed, Embase, Cochrane Library, and Web of Science, from their inception to July 2022. Pooled odds ratios (OR) with corresponding 95% confidence intervals (CI) were estimated using random effects models. Additionally, we conducted subgroup analyses to identify the potential sources of between-study heterogeneity. Sensitivity analysis was performed using the method of exclusion, one study at a time. Publication bias was examined using Egger’s test and funnel plot.ResultsA total of 14 studies from 13 articles including 4,793 participants were eligible for this meta-analysis. The outcome showed that vitamin D deficiency may increase the risk of BV during pregnancy by 54% (OR, 1.54; 95% CI, 1.25–1.91; P ConclusionThis meta-analysis showed that vitamin D deficiency is positively associated with the risk of BV during pregnancy. Further high-quality prospective cohort studies are needed to determine whether vitamin D intake reduces the prevalence of BV in pregnant women.</p
Data_Sheet_3_Vitamin D deficiency increases the risk of bacterial vaginosis during pregnancy: Evidence from a meta-analysis based on observational studies.PDF
BackgroundBacterial vaginosis (BV) is the most common microbiological syndrome in women of childbearing age, causing numerous adverse health issues in pregnant women. Several observational studies have discussed the association between vitamin D deficiency and the risk of BV during pregnancy, but the results were inconclusive. Therefore, this meta-analysis aimed to explore the association between vitamin D deficiency and BV risk in pregnant women.Materials and methodsWe searched four databases, including PubMed, Embase, Cochrane Library, and Web of Science, from their inception to July 2022. Pooled odds ratios (OR) with corresponding 95% confidence intervals (CI) were estimated using random effects models. Additionally, we conducted subgroup analyses to identify the potential sources of between-study heterogeneity. Sensitivity analysis was performed using the method of exclusion, one study at a time. Publication bias was examined using Egger’s test and funnel plot.ResultsA total of 14 studies from 13 articles including 4,793 participants were eligible for this meta-analysis. The outcome showed that vitamin D deficiency may increase the risk of BV during pregnancy by 54% (OR, 1.54; 95% CI, 1.25–1.91; P ConclusionThis meta-analysis showed that vitamin D deficiency is positively associated with the risk of BV during pregnancy. Further high-quality prospective cohort studies are needed to determine whether vitamin D intake reduces the prevalence of BV in pregnant women.</p
Data_Sheet_4_Vitamin D deficiency increases the risk of bacterial vaginosis during pregnancy: Evidence from a meta-analysis based on observational studies.PDF
BackgroundBacterial vaginosis (BV) is the most common microbiological syndrome in women of childbearing age, causing numerous adverse health issues in pregnant women. Several observational studies have discussed the association between vitamin D deficiency and the risk of BV during pregnancy, but the results were inconclusive. Therefore, this meta-analysis aimed to explore the association between vitamin D deficiency and BV risk in pregnant women.Materials and methodsWe searched four databases, including PubMed, Embase, Cochrane Library, and Web of Science, from their inception to July 2022. Pooled odds ratios (OR) with corresponding 95% confidence intervals (CI) were estimated using random effects models. Additionally, we conducted subgroup analyses to identify the potential sources of between-study heterogeneity. Sensitivity analysis was performed using the method of exclusion, one study at a time. Publication bias was examined using Egger’s test and funnel plot.ResultsA total of 14 studies from 13 articles including 4,793 participants were eligible for this meta-analysis. The outcome showed that vitamin D deficiency may increase the risk of BV during pregnancy by 54% (OR, 1.54; 95% CI, 1.25–1.91; P ConclusionThis meta-analysis showed that vitamin D deficiency is positively associated with the risk of BV during pregnancy. Further high-quality prospective cohort studies are needed to determine whether vitamin D intake reduces the prevalence of BV in pregnant women.</p
Table_1_Vitamin D deficiency increases the risk of bacterial vaginosis during pregnancy: Evidence from a meta-analysis based on observational studies.DOCX
BackgroundBacterial vaginosis (BV) is the most common microbiological syndrome in women of childbearing age, causing numerous adverse health issues in pregnant women. Several observational studies have discussed the association between vitamin D deficiency and the risk of BV during pregnancy, but the results were inconclusive. Therefore, this meta-analysis aimed to explore the association between vitamin D deficiency and BV risk in pregnant women.Materials and methodsWe searched four databases, including PubMed, Embase, Cochrane Library, and Web of Science, from their inception to July 2022. Pooled odds ratios (OR) with corresponding 95% confidence intervals (CI) were estimated using random effects models. Additionally, we conducted subgroup analyses to identify the potential sources of between-study heterogeneity. Sensitivity analysis was performed using the method of exclusion, one study at a time. Publication bias was examined using Egger’s test and funnel plot.ResultsA total of 14 studies from 13 articles including 4,793 participants were eligible for this meta-analysis. The outcome showed that vitamin D deficiency may increase the risk of BV during pregnancy by 54% (OR, 1.54; 95% CI, 1.25–1.91; P ConclusionThis meta-analysis showed that vitamin D deficiency is positively associated with the risk of BV during pregnancy. Further high-quality prospective cohort studies are needed to determine whether vitamin D intake reduces the prevalence of BV in pregnant women.</p
Synthesis and Characterization of Electron-Deficient Asymmetrically Substituted Diarylindenotetracenes
Electron-deficient
asymmetrically substituted diarylindenotetracenes
were prepared via a series of Friedel–Crafts acylations, aryl–aryl
cross-couplings, and an intramolecular oxidative cyclization to form
the indene ring. Single-crystal X-ray experiments showed good π–π
overlap with π–π distances ranging from 3.26 to
3.76 Ã…. Both thermogravimetric analysis and differential scanning
calorimetry indicated that asymmetrically substituted indenotetracenes
(ASIs) are stable at elevated temperatures. From cyclic voltammetry
experiments, HOMO/LUMO energy levels of ASI derivatives were determined
to be near −5.4/–4.0 eV. UV/visible absorption spectra
showed strong absorption of light between 400 and 650 nm with molar
attenuation coefficients from 10<sup>4</sup> to 10<sup>5</sup> M<sup>–1</sup> cm<sup>–1</sup>. ASIs were also found to have
very low fluorescence quantum yields, less than 4%. Using the solid-state
packing determined from the single-crystal X-ray experiments, computational
modeling indicated that ASI molecules should favor electron transport
Free-Standing Mn<sub>3</sub>O<sub>4</sub>@CNF/S Paper Cathodes with High Sulfur Loading for Lithium–Sulfur Batteries
Free-standing
paper cathodes with layer-by-layer structure are synthesized for high-loading
lithium–sulfur (Li–S) battery. Sulfur is loaded in a
three-dimensional (3D) interconnected nitrogen-doped carbon nanofiber
(CNF) framework impregnated with Mn<sub>3</sub>O<sub>4</sub> nanoparticles.
The 3D interconnected CNF framework creates an architecture with outstanding
mechanical properties. Synergetic effects generated from physical
and chemical entrapment could effectively suppress the dissolution
and diffusion of the polysulfides. Electrochemical measurements suggest
that the rationally designed structure endows the electrode with high
utilization of sulfur and good cycle performance. Specifically, the
cathode with a high areal sulfur loading of 11 mg cm<sup>–2</sup> exhibits a reversible areal capacity over 8 mAh cm<sup>–2</sup>. The fabrication procedure is of low cost and readily scalable.
We believe that this work will provide a promising choice for potential
practical applications
Cell-Based High-Throughput Screening Assay Identifies 2′,2′-Difluoro-2′-deoxycytidine Gemcitabine as a Potential Antipoliovirus Agent
As
we approach the global eradication of circulating wild-type polioviruses
(PV), vaccination with oral poliovirus vaccine (OPV) has led to the
emergence of circulating vaccine-derived poliovirus (cVDPV) and vaccine-associated
paralytic poliomyelitis (VAPP). Complete cessation of all poliovirus
infections may require stopping use of OPV and formulating improved
vaccines and new antiviral drugs. Currently, no licensed drugs are
available to treat chronically infected poliovirus excretors. Here,
we created a modified PV expressing Gaussia Luciferase (Sb-Gluc) and
developed a cell-based high-throughput screening (HTS) antiviral assay.
Using the validated HTS assay, we screened the FDA-approved drug library
of compounds and identified candidate agents capable of inhibiting
PV replication. We then characterized antipoliovirus activity for
the best hit, gemcitabine, a nucleoside analogue used in tumor chemotherapy.
We found that gemcitabine inhibited PV Mahoney replication with an
IC<sub>50</sub> of 0.3 μM. It completely protected HeLa cells
from PV-induced cytopathic effects at 25 μM, without detectable
toxicity for cell viability. Furthermore, a gemcitabine metabolite
directly inhibited the ability of PV RNA polymerase to synthesize
or elongate PV RNA. Because PV RNA polymerase is somehow conserved
among species in the <i>Picornaviridae</i> family, gemcitabine
may be further developed as an attractive broad-spectrum antiviral
for PV and others
Partial Fluorination as a Strategy for Crystal Engineering of Rubrene Derivatives
Through
a close examination of the intermolecular interactions
of rubrene (<b>1a</b>) and select derivatives (<b>1b</b>–<b>1p</b>), a clearer understanding of why certain
fluorinated rubrene derivatives pack with planar tetracene backbones
has been achieved. In this study we synthesized, crystallized, and
determined the packing structure of new rubrene derivatives (<b>1h</b>–<b>p</b>). Previously, we proposed that introducing
electron-withdrawing CF<sub>3</sub> substituents induced planarity
by reducing intramolecular repulsion between the peripheral aryl groups
(<b>1e</b>–<b>g</b>). However, we found that in
most cases, further increasing the fluorine content of rubrene lead
to twisted tetracene backbones in the solid state. To understand how
rubrene (<b>1a</b>) and its derivatives (<b>1b</b>–<b>p</b>) pack in the solid state, we (re)Âexamined the crystal structures
through a systematic study of the close contacts. We found that planar
tetracene cores occur when close contacts organize to produce an <i>S</i> symmetry element about a given rubrene molecule. We report
the first instance of rubrene derivatives (<b>1l</b> and <b>1n</b>) that pack in a two-dimensional brick motif. The prospects
for new rubrene derivatives in semiconductors were estimated by calculating
the reorganization energies of the monomers and transfer integrals
of the dimers we observed. Our work allows for the rational design
and improved crystal engineering of new rubrene derivatives