A REEXAMINATION OF THE ROBUSTNESS OF THE FRAMING EFFECT IN COGNITIVE PROCESSING

We conducted 2 experiments on the framing effect; recording reaction times in the first using a questionnaire and in the second using a computer-programmed procedure. We found that a positive framing effect caused participants to make faster decisions and show an intuitive, heuristic, decision-making pattern; whereas a negative framing effect caused participants to make slower decisions and show a rational, analytical, decision-making pattern. These results suggest that when decision-making time is not strictly controlled, other potential factors such as individual and cultural differences may influence the robustness of the framing effect

A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity

<p>Abstract</p> <p>Background</p> <p>Influenza A virus can infect a wide variety of animal species including humans, pigs, birds and other species. Viral ribonucleoprotein (vRNP) was involved in genome replication, transcription and host adaptation. Currently, firefly luciferase (Fluc) reporter system was used in vRNP functional assay. However, its limitation for the testing by virus infection resulted in an increased need for rapid, sensitive, and biosafe techniques. Here, an influenza A virus UTR-driven gene reporter for vRNP assay based on secreted <it>Gaussia </it>luciferase (Gluc) activity was evaluated.</p> <p>Results</p> <p>By measuring Gluc levels in supernatants, reporter gene activity could be detected and quantitated after either reconstitution of influenza A virus polymerase complex or viral infection of 293T and A549 cells, respectively. As compared with Fluc reporter, Gluc-based reporter was heat-tolerant (65°C for 30 min) and produced 50-fold higher bioluminescent activity at 24 h posttransfection. Signals generated by Gluc reporter gene could be detected as early as 6 h post-infection and accumulated with time. Testing by viral infection, stronger signals were detected by Gluc reporter at a MOI of 0.001 than that of 1 and the effects of PB2-627K/E or amantadine on influenza vRNP activity were elucidated more effectively by the Gluc reporter system.</p> <p>Conclusions</p> <p>This approach provided a rapid, sensitive, and biosafe assay of influenza vRNP function, particularly for the highly pathogenic avian influenza viruses.</p

Who Is the Rightful Owner? Young Children’s Ownership Judgments in Different Transfer Contexts

This study aimed to examine whether Chinese preschoolers understand that ownership can be transferred in different contexts. The study participants were 3- to 5-year-old Chinese children (n = 96) and adults (n = 34). With four scenarios that contained different transfer types (giving, stealing, losing, and abandoning), participants were asked four questions about ownership. The results indicated that preschoolers’ ability to distinguish legitimate ownership transfers from illegitimate ownership transfers improved with age. Three-year-olds understood that ownership cannot be transferred in a stealing context, but the appropriate understanding of ownership was not attained until 4 years old in a giving context and 5 years old in losing and abandoning contexts, which is similar to the adults’ performance. In addition to the first possessor bias (a tendency to judge the first possessor as the owner) found in previous studies, 3-year-olds also displayed a loan bias (a tendency to believe everything that is transferred should be returned) in the study. The findings suggest that the developmental trajectories of preschoolers’ understanding of ownership transfers varied across different contexts, which may relate to children’s ability to consider the role of intent in determining ownership and parents’ disciplinary behavior. Both cross-cultural similarities and differences are discussed

Aqueous electrosynthesis of an electrochromic material based water-soluble EDOT-MeNH2 hydrochloride

2\u27-Aminomethyl-3,4-ethylenedioxythiophene (EDOT-MeNH2) showed unsatisfactory results when its polymerization occurred in organic solvent in our previous report. Therefore, a water-soluble EDOT derivative was designed by using hydrochloric modified EDOT-MeNH2 (EDOT-MeNH2·HCl) and electropolymerized in aqueous solution to form the corresponding polymer with excellent electrochromic properties. Moreover, the polymer was systematically explored, including electrochemical, optical properties and structure characterization. Cyclic voltammetry showed low oxidation potential of EDOT-MeNH2·HCl (0.85 V) in aqueous solution, leading to the facile electrodeposition of uniform the polymer film with outstanding electroactivity. Compared with poly(2′-aminomethyl- 3,4-ethylenedioxythiophene) (PEDOT-MeNH2), poly(2′-aminomethyl-3,4-ethylenedioxythiophene salt) (PEDOT-MeNH3 +A-) revealed higher efficiencies (156 cm2 C-1), lower bandgap (1.68 eV), and faster response time (1.4 s). Satisfactory results implied that salinization can not only change the polymerization system, but also adjust the optical absorption, thereby increase the electrochromic properties

Testing the knowledge of Alzheimer's disease via an intervention study among community health service center staff in Jiaxing, China

ObjectiveThis study aimed to investigate the knowledge status of Alzheimer's disease (AD) among community health service center (CHSC) staff in Jiaxing, China, and to compare the effects of online with offline training.MethodsA total of 763 people from 12 community health service centers were investigated using a self-created general situation questionnaire and the Alzheimer's Disease Knowledge Scale (ADKS). Among the participants, 261 people who were willing to receive training were randomly divided into two groups according to the institution in which they worked to receive online or offline training, respectively.ResultsThe average ADKS score was 19.77, and the awareness rate was 65.92%; the results for every field were as follows: treatment and management (81.32%); life impact (77.76%); disease course (75.23%); assessment and diagnosis (68.94%); risk factors (65.05%); symptoms (57.90%); caregiving (44.06%). Education and profession had impacts on the total ADKS scores (P &lt; 0.05). A total of 261 people participated in the training, and there were significant differences in ADKS scores before and after training (P &lt; 0.05). Before the training, there was no significant difference in ADKS score between the two groups; after the training, either (P &gt; 0.05). There were significant differences in the ADKS scores after training in both groups (P &lt; 0.05).ConclusionCommunity health service center staff in Jiaxing had limited knowledge of AD, particularly in the “symptom” and “caregiving” dimensions. One instance of training on AD-related knowledge to some degree helped to improve this but still fell short of meeting the national requirements. No significant differences were found between offline and online training effects