An Experimental Study on the Establishment of Pulmonary Hypertension Model in Rats induced by Monocrotaline

Pulmonary hypertension is called PH for short. It is caused by the pulmonary artery vascular disease leading to pulmonary vascular resistance, and the increase right lung compartment load, which resulting in weakening or even collapse of the right ventricular function. The establishment of rat PH model under the action of monocrotaline is a repeatable, simple and accessible operation technique, which has been widely used in the treatment of pulmonary hypertension. This paper discusses the principle and properties of the PH model on rats under the monocrotaline action

Neutrophil extracellular traps induce IL-1β production by macrophages in combination with lipopolysaccharide

Upon exposure to invading microorganisms, neutrophils undergo NETosis, a recently identified type of programmed cell death, and release neutrophil extracellular traps (NETs). NETs are described as an antimicrobial mechanism, based on the fact that NEls can trap microorganisms and exhibit bactericidal activity through the action of NET-associated components. In contrast, the components of NETs have been recognized as damage-associated molecular pattern molecules (DAMPs), which trigger inflammatory signals to induce cell death, inflammation and organ failure. In the present study, to clarify the effect of NETs on cytokine production by macrophages, mouse macrophage-like J774 cells were treated with NETs in combination with lipopolysaccharide (LPS) as a constituent of pathogen-associated molecular patterns. The results revealed that NETs significantly induced the production of interleukin (IL)-1 beta by J774 cells in the presence of LPS. Notably, the NET/LPS-induced IL-1 beta production was inhibited by both caspase-1 and caspase-8 inhibitors. Furthermore, nucleases and serine protease inhibitors but not anti-histone antibodies significantly inhibited the NET/LPS-induced IL-1 beta production. Moreover, we confirmed that caspase-1 and caspase-8 were activated by NETs/LPS, and the combination of LPS, DNA and neutrophil elastase induced IL-1 beta production in reconstitution experiments. These observations indicate that NETs induce the production of IL-1 beta by J774 macrophages in combination with LPS via the caspase-1 and caspase-8 pathways, and NET-associated DNA and serine proteases are involved in NET/LPS-induced IL-1 beta production as essential components

Antimicrobial cathelicidin peptide LL-37 inhibits the pyroptosis of macrophages and improves the survival of polybacterial septic mice

LL-37 is the only known member of the cathelicidin family of antimicrobial peptides in humans. In addition to its broad spectrum of antimicrobial activities, LL-37 can modulate various inflammatory reactions. We previously revealed that LL-37 suppresses the LPS/ATP-induced pyroptosis of macrophages in vitro by both neutralizing the action of LPS and inhibiting the response of P2X(7) (a nucleotide receptor) to ATP. Thus, in this study, we further evaluated the effect of LL-37 on pyroptosis in vivo using a cecal ligation and puncture (CLP) sepsis model. As a result, the intravenous administration of LL-37 improved the survival of the CLP septic mice. Interestingly, LL-37 inhibited the CLP-induced caspase-1 activation and pyroptosis of peritoneal macrophages. Moreover, LL-37 modulated the levels of inflammatory cytokines (IL-1 beta, IL-6 and TNF-alpha) in both peritoneal fluids and sera, and suppressed the activation of peritoneal macrophages (as evidenced by the increase in the intracellular levels of IL-1 beta, IL-6 and TNF-alpha). Finally, LL-37 reduced the bacterial burdens in both peritoneal fluids and blood samples. Together, these observations suggest that LL-37 improves the survival of CLP septic mice by possibly suppressing the pyroptosis of macrophages, and inflammatory cytokine production by activated macrophages and bacterial growth. Thus, the present findings imply that LL-37 can be a promising candidate for sepsis because of its many functions, such as the inhibition of pyroptosis, modulation of inflammatory cytokine production and antimicrobial activity

Antimicrobial cathelicidin peptide LL-37 inhibits the pyroptosis of macrophages and improves the survival of polybacterial septic mice

LL-37 is the only known member of the cathelicidin family of antimicrobial peptides in humans. In addition to its broad spectrum of antimicrobial activities, LL-37 can modulate various inflammatory reactions. We previously revealed that LL-37 suppresses the LPS/ATP-induced pyroptosis of macrophages in vitro by both neutralizing the action of LPS and inhibiting the response of P2X(7) (a nucleotide receptor) to ATP. Thus, in this study, we further evaluated the effect of LL-37 on pyroptosis in vivo using a cecal ligation and puncture (CLP) sepsis model. As a result, the intravenous administration of LL-37 improved the survival of the CLP septic mice. Interestingly, LL-37 inhibited the CLP-induced caspase-1 activation and pyroptosis of peritoneal macrophages. Moreover, LL-37 modulated the levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α) in both peritoneal fluids and sera, and suppressed the activation of peritoneal macrophages (as evidenced by the increase in the intracellular levels of IL-1β, IL-6 and TNF-α). Finally, LL-37 reduced the bacterial burdens in both peritoneal fluids and blood samples. Together, these observations suggest that LL-37 improves the survival of CLP septic mice by possibly suppressing the pyroptosis of macrophages, and inflammatory cytokine production by activated macrophages and bacterial growth. Thus, the present findings imply that LL-37 can be a promising candidate for sepsis because of its many functions, such as the inhibition of pyroptosis, modulation of inflammatory cytokine production and antimicrobial activity

Effect of LL-37 on the LPS/ATP-induced inflammasome formation in J774 cells.

<p>J774 cells were primed with 10/ml LPS for 4 h, and then treated with 3 mM ATP for 90 min in the absence or presence of LL-37 (1 µg/ml). Cells were also incubated with LPS or ATP alone, or without LPS and ATP (Resting). Thereafter, the cells were stained with FAM-YVAD-fmk (a fluorescent labeled caspase-1 inhibitor) and Hoechst 33342 (A). Arrowheads indicate the inflammasomes containing the activated caspase-1. Furthermore, the percentage of inflammasome-containing cells with activated caspase-1 was determined by counting at least 200 Hoechst positive cells (B). Data shows the mean ± SD of 3-5 separate experiments. Values are compared in the LPS/ATP-treated cells between the absence and presence of LL-37. ***P<0.001. Images cells are representative of 3–5 separate experiments.</p

Antimicrobial Cathelicidin Peptide LL-37 Inhibits the LPS/ATP-Induced Pyroptosis of Macrophages by Dual Mechanism

<div><p>Pyroptosis is a caspase-1 dependent cell death, associated with proinflammatory cytokine production, and is considered to play a crucial role in sepsis. Pyroptosis is induced by the two distinct stimuli, microbial PAMPs (pathogen associated molecular patterns) and endogenous DAMPs (damage associated molecular patterns). Importantly, cathelicidin-related AMPs (antimicrobial peptides) have a role in innate immune defense. Notably, human cathelicidin LL-37 exhibits the protective effect on the septic animal models. Thus, in this study, to elucidate the mechanism for the protective action of LL-37 on sepsis, we utilized LPS (lipopolysaccharide) and ATP (adenosine triphosphate) as a PAMP and a DAMP, respectively, and examined the effect of LL-37 on the LPS/ATP-induced pyroptosis of macrophage-like J774 cells. The data indicated that the stimulation of J774 cells with LPS and ATP induces the features of pyroptosis, including the expression of IL-1β mRNA and protein, activation of caspase-1, inflammasome formation and cell death. Moreover, LL-37 inhibits the LPS/ATP-induced IL-1β expression, caspase-1 activation, inflammasome formation, as well as cell death. Notably, LL-37 suppressed the LPS binding to target cells and ATP-induced/P2X₇-mediated caspase-1 activation. Together these observations suggest that LL-37 potently inhibits the LPS/ATP-induced pyroptosis by both neutralizing the action of LPS and inhibiting the response of P2X₇ to ATP. Thus, the present finding may provide a novel insight into the modulation of sepsis utilizing LL-37 with a dual action on the LPS binding and P2X₇ activation.</p></div

Effect of LL-37 on the ATP-induced caspase-1 activation in J774 cells.

<p>J774 cells were treated with 3-37 (0.01, 0.1 or 1 µg/ml), P2X₇ antagonists (1 µM KN-62 and 1 µM KN-93) or dimethylsulfoxide (DMSO, a solvent for KN-62 and KN-93, 0.1%). Cells were also incubated without ATP, LL-37 and P2X₇ antagonists (Resting). Thereafter, the caspase-1 activation was assayed by flow cytometry using FAM-YVAD-fmk (FLICA), and expressed as the percentage of FLICA positive cells. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of LL-37 or P2X₇ antagonists among ATP-treated cells. **P<0.01, ***P<0.001.</p

Effect of LL-37 on the LPS binding to J774 cells.

<p>J774 cells were suspended in DMEM containing 10% FBS, and incubated with 1 µg/ml FITC-LPS at 37°C for 15 min in the absence or presence of LL-37 (0.01, 0.1 or 1 µg/ml), anti-mouse CD14 monoclonal antibody (4C1, 10 µg/ml), anti-mouse TLR4 monoclonal antibody (MTS510, 40 µg/ml) or isotype control IgG (IgG2b and IgG2a). The binding of LPS was analyzed by flow cytometry, and the median fluorescence intensity was determined. The LPS binding was expressed as the percentage of that with FITC-LPS alone. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of LL-37, anti-CD14 monoclonal antibody or anti-TLR4 monoclonal antibody. **P<0.01, ***P<0.001.</p

Effect of LPS and ATP treatment on the pyroptosis of J774 cells.

<p>Macrophage-like J774 cells were primed with 10 ng/ml LPS for 4 h, and then treated with 3 mM ATP for the indicated time periods in the absence or presence of 20 µM Ac-YVAD-CHO, a caspase-1 specific inhibitor. Cells were also incubated with LPS or ATP alone, or without LPS and ATP (Resting). Thereafter, the supernatants were recovered for the assays of IL-1β (A) and LDH (B). IL-1β 1evels were determined using a commercially available mouse IL-1β ELISA kit. LDH activities in the supernatants and 1% Triton X-100-lysed cells (as a total activity of 100%) were determined using a commercially available LDH assay kit. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of Ac-YVAD-CHO. **P<0.01, ***P< 0.001. (C) J774 cells were primed with 10 ng/ml LPS for 4 h and then treated with 3 mM ATP for 90 min. Thereafter, the cells were stained with FAM-YVAD-fmk (a fluorescent labeled caspase-1 inhibitor for inflammasome staining, green) and Hoechst 33342 (for nuclear staining, blue) and photographed with a fluorescence microscope system. Arrowheads indicate the inflammasomes containing the activated caspase-1. Images of cells are representative of 3 separate experiments.</p

Effect of LL-37 on the LPS/ATP-induced pyroptosis of J774 cells.

<p>J774 cells were primed with 10/ml LPS for 4 h, and then treated with 3 mM ATP for 90 min in the absence or presence of LL-37 (0.01, 0.1 or 1 µg/ml). Cells were also incubated with LPS or ATP alone, or without LPS and ATP. Thereafter, the supernatants were recovered for the assays of IL-1β (A) and LDH (C), and the cells were used for the assays of IL-1β mRNA expression (B) and caspase-1 activation (D). IL-1β mRNA expression were determined by RT-PCR and expressed as fold increase relative to resting cells incubated without LPS, ATP and LL-37; the caspase-1 activation was assayed by flow cytometry using FAM-YVAD-fmk (a fluorescent labeled inhibitor of caspase-1, FLICA) that irreversibly binds with activated caspase-1, and expressed as the percentage of FLICA positive cells. Data shows the mean ± SD of 3-5 separate experiments. Values are compared between the absence and presence of LL-37 among LPS/ATP-treated cells. *P<0.05, **P<0.01, ***P<0.001. Images of RT-PCR are representative of 3-5 separate experiments.</p