Assessing the in vivo ameliorative effects of Lactobacillus acidophilus KLDS1.0901 for induced non-alcoholic fatty liver disease treatment

Reputed as a significant metabolic disorder, non-alcoholic fatty liver disease (NAFLD) is characterized by high-fat deposits in the liver and causes substantial economic challenges to any country's workforce. Previous studies have indicated that some lactic acid bacteria may effectively prevent or treat NAFLD. Overall, L. acidophilus KLDS1.0901 protected against HFD-induced NAFLD by improving liver characteristics and modulating microbiota composition, and thus could be a candidate for improving NAFLD. This study aimed to assess the protective effects of L. acidophilus KLDS1.0901 on a high-fat diet(HFD)-induced NAFLD. First, hepatic lipid profile and histological alterations were determined to study whether L. acidophilus KLDS1.0901 could ameliorate NAFLD. Then, the intestinal permeability and gut barrier were explored. Finally, gut microbiota was analyzed to elucidate the mechanism from the insights of the gut–liver axis. The results showed that Lactobacillus KLDS1.0901 administration significantly decreased body weight, Lee's index body, fat rate, and liver index. L. acidophilus KLDS1.0901 administration significantly improved lipid profiles by decreasing the hepatic levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) and by increasing the high-density lipoprotein cholesterol (HDL-C) levels. A conspicuous decrease of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum was observed after L. acidophilus KLDS1.0901 administration. Meanwhile, the H&E and Oil Red O-stained staining showed that L. acidophilus KLDS1.0901 significantly reduced liver lipid accumulation of HFD-fed mice by decreasing the NAS score and lipid area per total area. Our results showed that L. acidophilus KLDS1.0901 administration decreased the interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α) concentrations accompanied by the increase of interleukin-10 (IL-10). L. acidophilus KLDS1.0901 administration could improve the intestinal barrier function by upregulating the mRNA levels of occludin, claudin-1, ZO-1, and Muc-2, which were coupled to the decreases of the concentration of LPS and D-lactic acid. Notably, L. acidophilus KLDS1.0901 administration modulated the gut microbiota to a near-normal pattern. Hence, our results suggested that L. acidophilus KLDS1.0901 can be used as a candidate to ameliorate NAFLD

Determination of the major axis direction and macroseismic epicenter of the assessed intensity map based on relocated aftershock sequences

The rapid assessment of an intensity map following a strong earthquake forms the foundation for risk assessment and emergency response in Chinese mainland. The determination of the major axis direction in the assessed intensity map depends on various factors, including nearby active faults, instrumental intensities, and the distribution of aftershocks. To evaluate the effectiveness of promptly determining the major axis direction based on early aftershock distribution, we relocated and analyzed aftershock sequences occurring within 2 h of eighteen aftershock-rich earthquakes with a magnitude of Ms ≥ 6.0 in Chinese mainland from 2012 to 2021. HypoDD was used for relocation, and the standard deviational ellipse fitting technique was employed for analysis. Comparing the aftershock ellipses resulting from our analysis with the macroseismic intensity maps obtained from field surveys reveals a high level of agreement, with an average difference of approximately 9° in the major axis directions of the aftershock ellipses and the meizoseismal zones. For the majority of earthquakes, regardless of focal mechanisms, the lengths of the major axes of the aftershock ellipses closely correspond to the intensity VIII scale. Additionally, the spatial distribution of aftershocks aids in distinguishing the seismogenic fault from the two fault planes with distinctly different dips as indicated by the focal mechanism solution. Moreover, the distance between the aftershock center and the macroseismic epicenter systematically increases as the dip decreases. These findings hold significant scientific value as they contribute to the prompt determination of assessed intensity maps and provide effective guidance for earthquake emergency response

Facile Fluorescence "Turn on" Sensing of Lead Ions in Water via Carbon Nanodots Immobilized in Spherical Polyelectrolyte Brushes

Heavy metal detection has become very important for the protection of water resource. In this work, a novel controllable probe is presented for the sensitive detection of Pb2+ in aqueous solutions. The probe was synthesized via the immobilization of surface functionalized carbon dots (named as CAEA-Hs) into the shell of the spherical polyelectrolyte brushes (SPB). The fluorescence of CAEA-H was firstly "turned off" via electrostatic interaction induced quenching. Based on the aggregation induced emission enhancement (AIEE), the fluorescence of the immobilized CAEA-H could be specifically turned on via the aggregation of the SPB particles. This fluorescence "turn on" sensor could selectively detect Pb2+ among five different metal ions with a relatively wide detecting range (0-1.67 mM) and good linear relationship (R 2 = 0.9958). Moreover, the aggregating behavior and nano-structure of CAEA-H loaded SPB have been systematically analyzed via small angle X-ray scattering, turbidity titration, and Zeta-potential measurement. Based on a series of control experiments, we finally gain an insight into the sensing mechanism of this novel sensing probe. This contributed a proof of concept demonstration that sensitive and selective chemical detection can be achieved via a C-dot/SPB synergistic platform

Effect of the Preheating Strategy on the Combustion Process of the Intake Manifold Burner

The intake air preheating is an effective method to improve the cold start performance of diesel engines. The combustion process and ignition probability were investigated in the present study. The average flame area (AFA) during the steady stage of the combustion process was used to evaluate the effects of various factors on combustion. The increase of voltage was found to enhance the combustion process, while the increased diesel flow rate first promoted the combustion before deteriorating it. The increased intake air flow velocity enhanced the combustion within 2.64 m/s, and excessive air flow velocity hindered the combustion from 2.7 to 3 m/s. The cross-distributed vortex clusters in the combustion chamber, periodic diesel evaporation and vortexes with opposite rotation directions in the vicinity of the intake manifold burner were believed to be the main reasons for flame stripping and swirl motion. The temperature rise in the exhaust pipe was recorded to investigate the thermal distribution. The warm air was concentrated in the upper region because of the buoyancy effect of the flame. With the air flow velocity increasing from 1.4 to 10 m/s, the average temperature rise increased first before decreasing, while the combustion efficiency increased due to the increased air flow volume

Compressibility and thermal expansion of natural clinopyroxene Di0.66Hd0.13Jd0.12Ts0.05

A natural clinopyroxene (cpx) has been experimentally studied by in-situ X-ray powder diffraction combined with an externally heated diamond anvil cell (DAC) technique in the temperature range 300–673 K and at pressures up to 15 GPa. The specimen has a composition of Di0.66Hd0.13Jd0.12Ts0.05 on the basis of four end-members, diopside (CaMgSi2O6), hedenbergite (CaFeSi2O6), jadeite (NaAlSi2O6), and Mg-Tschermak (MgAl(AlSi)O6). By combining the current data with literature results, an empirical relationship, V0 (Å3) = 392.5(6.2) + 46.7(6.5) × Di + 57.6(6.3) × Hd + 9.9(6.2) × Jd has been obtained to describe the relationship between the unit-cell volume and the composition of Di-Jd-Hd solid solution. The isothermal bulk modulus KT0 = 112(3) GPa and its pressure derivative K′T0 = 5.0(7) are obtained by fitting pressure-volume data with a 3rd-order Birch-Murnaghan equation of state (B-M EoS). The volume thermal expansion coefficients are determined to be α0 = 2.97(8) × 10−5/K and 2.82(2) × 10−5/K at ∼6.9(2) GPa and 8.5(2) GPa, respectively, from which ∂α/∂P = −9.3 × 10−7/K GPa−1 and ∂KT/∂T = −0.012 GPa/K are resulted for the current Di0.66Hd0.13Jd0.12Ts0.05. Keywords: Clinopyroxene, X-ray diffraction, Diamond anvil cell, Equation of state, Thermal expansio

Effect of the Air Flow on the Combustion Process and Preheating Effect of the Intake Manifold Burner

Diesel engines show poor performance and high emissions under cold-start conditions. The intake manifold burner is an effective method to increase the intake air temperature and improve engine performance. In this paper, a visualization system was employed to investigate the combustion process of the intake manifold burner. The effects of diesel flow rate and airflow velocity on combustion performance were investigated. The combustion process of the intake manifold burner showed four stages: preparing stage A, rapid development stage B, steady-development stage C, and stable stage D. Flame stripping was found in stages C and D, presenting the instability of the combustion process. With the increase in air flow velocity from 1.4 m/s to 3.0 m/s, the flame stripping was enhanced, leading to the increasing combustion instability and regular flame penetration fluctuations. The average temperature rise and combustion efficiency increased with the increasing diesel flow rate, indicating the combustion enhancement. Comparison of temperature rise and combustion efficiency under 2.0 m/s and 10.0 m/s showed that stronger cross wind enhances the heat convection, improving the temperature uniformity and combustion efficiency

Complicated Tumor Lysis Syndrome after CVVH Treatment in a Renal Transplantation Patient: One Case Report and Literature Review

Tumor lysis syndrome (TLS) is a potentially lethal emergency causedby lysed tumor cells, and it frequently occurs in tumors of hematologic origin. Up until now, there has been only one known reportpublished overseas about TLS resulting from post-transplant lymphoproliferative disorder (PTLD)[1]. In 2008, a PTLD patient whohad previously undergone renal transplantation was treated in therenal transplantation center of our hospital, and TLS occurred duringtreatment for PTLD. At the time, no further literature regarding thetreatment of post-transplant renal inadequacy complicating TLS wasavailable. So, continuous veno-venous hemofiltration (CVVH) wasused to effectively control the progression of TLS. In this article, the experiences gained from our management of this complex case andour analysis, supported by relevant literature, are described

A bidirectional DC-DC converter with high voltage conversion ratio and zero ripple current for battery energy storage system

In this article, a novel bidirectional dc-dc converter (BDC) consisting of an active switched-inductor (A-SL) cell, a zero current ripple cell and an auxiliary capacitor cell is proposed for the battery energy storage system. The proposed BDC integrates with the advantages of high voltage conversion ratio, low power switch voltage stresses, zero ripple current on the low voltage side (LVS), and constant potential difference between the grounds of LVS and high voltage side. Thanks to the use of synchronous rectification technology, the efficiency of the proposed BDC is increased. The operating principles, the characteristics analysis and the parameter design of the proposed BDC are given. In addition, to show the merits of the proposed BDC, a comparison study involves the proposed converter and other BDCs is demonstrated. Finally, some experimental results obtained using a 1000 W prototype are presented to valid the effectiveness of the proposed BDC. 96.43% and 96.67% maximum conversion efficiencies can be achieved in the step-up and step-down modes, respectively.This work was supported by the Natural Science Foundation of China under Grant 51977145