Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

Salt Overly Sensitive 1 (SOS1) is one of the members of the Salt Overly Sensitive (SOS) signaling pathway and plays critical salt tolerance determinant in plants, while the characterization of the SOS1 family in potato (Solanum tuberosum) is lacking. In this study, 37 StSOS1s were identified and found to be unevenly distributed across 10 chromosomes, with most of them located on the plasma membrane. Promoter analysis revealed that the majority of these StSOS1 genes contain abundant cis-elements involved in various abiotic stress responses. Tissue specific expression showed that 21 of the 37 StSOS1s were widely expressed in various tissues or organs of the potato. Molecular interaction network analysis suggests that 25 StSOS1s may interact with other proteins involved in potassium ion transmembrane transport, response to salt stress, and cellular processes. In addition, collinearity analysis showed that 17, 8, 1 and 5 of orthologous StSOS1 genes were paired with those in tomato, pepper, tobacco, and Arabidopsis, respectively. Furthermore, RT-qPCR results revealed that the expression of StSOS1s were significant modulated by various abiotic stresses, in particular salt and abscisic acid stress. Furthermore, subcellular localization in Nicotiana benthamiana suggested that StSOS1-13 was located on the plasma membrane. These results extend the comprehensive overview of the StSOS1 gene family and set the stage for further analysis of the function of genes in SOS and hormone signaling pathways

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Vertical Structure and Seasonal Variability of Shear on the Southwestern Continental Slope of the East China Sea

The vertical structure and seasonal variability of shear were examined using nearly three years of mooring ADCP (acoustic Doppler current profiler) data on the southwestern continental slope of the East China Sea (ECS). Shear spectra suggest that the sub-inertial currents (SICs); near-inertial waves (NIWs); and diurnal (D1), semidiurnal (D2), and tridiurnal (D3) internal tides (ITs) dominate the local shear field. The shear exhibits a remarkable surface-intensified pattern with high values occurring mostly in the upper 200 m. Significant seasonal variations can be found in the shear, but with differences between the upper (50–200 m averaged) and lower layers (210–570 m averaged). Satellite altimeter data indicate that the meander of the Kuroshio mainstream and the Kuroshio intrusion affect the seasonal variation of total shear by mainly influencing the shear caused by SICs. In addition, the shear efficiency (SE) of D2 ITs is obviously less than that of NIWs and that of D1 and D3 ITs via analyzing the kinetic energy (KE) densities and shear caused by these motions, since the predominant mode of the former is the first baroclinic mode, while the latter is dominated by higher baroclinic modes with large vertical wavenumbers. Moreover, the SE of incoherent ITs is relatively stronger than that of coherent ITs as a result of a larger proportion of high baroclinic modes in the incoherent component compared to the coherent component, based on modal decomposition

Observation of Near-Inertial Waves Induced by Typhoon Mitag (2019) on the Southwestern East China Sea Continental Slope

Based on horizontal velocity data recorded by a moored acoustic Doppler current profiler (ADCP) deployed on the southwestern continental slope of the East China Sea (ECS), this study investigates the characteristics of near-inertial waves (NIWs) induced by typhoon Mitag in October 2019. The results indicated that Mitag-induced near-inertial kinetic energy (NIKE) was mainly concentrated above 290 m and was subsurface-intensified; both the maximum velocity and kinetic energy of the NIWs occurred at a depth of 100 m and were 0.21 m/s and 23.01 J/m3, respectively. The rotary vertical wavenumber spectra suggested that both downward and upward energy propagation existed. However, upward energy propagation was much smaller than downward energy propagation, mainly in the 0.007–0.014 cpm wavenumber band. The NIWs had an e-folding timescale of 9.5 days and were red-shifted as a result of the Doppler shift of the Kuroshio. Normal mode analysis suggested that the NIWs were dominated by the first and fourth baroclinic modes, which together accounted for 76.7% of the total NIKE. Spectral analysis showed that although the spectral density of the semidiurnal internal tide (M2) peak overwhelmed that of the NIWs by a factor of approximately 30, the shear strength generated by the NIWs was comparable to that of the semidiurnal internal tide (M2), which plays an important role in upper ocean mixing on the southwestern continental slope of the ECS. In addition, the bicoherence analysis suggested that a harmonic wave (M2–f) was generated via the nonlinear interaction between the NIWs and semidiurnal internal tide (M2), which reflects the energy dissipation mechanism of semidiurnal tides and NIWs on the southwestern continental slope of the ECS

Plasmon-Induced Transparency for Tunable Atom Trapping in a Chiral Metamaterial Structure

Plasmon-induced transparency (PIT), usually observed in plasmonic metamaterial structure, remains an attractive topic for research due to its unique optical properties. However, there is almost no research on using the interaction of plasmonic metamaterial and high refractive index dielectric to realize PIT. Here, we report a novel nanophotonics system that makes it possible to realize PIT based on guided-mode resonance and numerically demonstrate its transmission and reflection characteristics by finite element method simulations. The system is composed of a high refractive-index dielectric material and a two-dimensional metallic photonic crystal with 4-fold asymmetric holes. The interaction mechanism of the proposed structure is analyzed by the coupled-mode theory, and the effects of the parameters on PIT are investigated in detail. In addition, we first consider this PIT phenomenon of such fields on atom trapping (87Rb), and the results show that a stable 3D atom trapping with a tunable range of position of about ~17 nm is achieved. Our work provides a novel, efficient way to realize PIT, and it further broadens the application of plasmonic metamaterial systems

Discovering the evolution of resource-based theory: science mapping based on bibliometric analysis

A great number of papers published on resource-based theory (RBT) have shaped its popularity and usefulness as a strategic approach. This study contributes to the literature by reviewing and evaluating the development of RBT research. Using a bibliometric analysis, we analyzed 2771 primary focal articles published between 1991 and 2020 and 103,796 secondary references. Our research reveals the status of RBT research by identifying the influential publications, authors, countries, institutions, and journals in this field. Based on the bibliometric analysis results, we suggest the future agenda of RBT research. Complementing previous qualitative literature reviews and quantitative meta-analyses, our study provides a systematic and dynamic understanding of RBT research

Influence of soil properties and aging on exogenous antimony toxicity to Caenorhabditis elegans in agricultural soil

Exploring the influence of soil on antimony (Sb) aging could help predict Sb toxicity on nematodes that play an important role in agricultural soil nitrogen cycling. This study aimed to investigate the major soil factors affecting the aging process and toxicity of exogenous Sb. Therefore, nematodes were exposed to varying levels of Sb contamination (0–6400 mg/kg) in nine agricultural soils, with aging periods of 7, 56, and 168 days, under dark conditions at 20 ± 0.5 °C for 96 h. The results suggested that nematode reproduction was more sensitive to the toxicity of exogenous trivalent Sb (Sb(III)) compared to growth and fertility. Following 7–168 days of aging, the EC50 of nematode reproduction increased from 546–1557 to 3560–6193 mg/kg in nine soils contaminated by exogenous Sb(III). Exogenous Sb(III) toxicity is overestimated without considering its aging process. The aging factors (AF) of nine soils aged over 7–168 days were calculated as 3.54–8.03. The regression equation AF = 0.923 pH − 0.812 (n = 9, adjust-r2 = 0.687, P = 0.004) indicated that pH was the primary soil factor explaining 85.2% of the variance in the aging process of exogenous Sb(III). No significant toxicity was observed in soils contaminated with exogenous pentavalent Sb after 7 days of aging. These findings could provide guidance for the adjustment of Sb toxicity data, the revision of soil environmental quality standard, and efficient soil environmental management.</p

The Critical Shoulder Angle Can be Accurately and Reliably Determined from Three‐Dimensional Computed Tomography Images

Objective Anteroposterior (AP) radiographs do not necessarily offer the optimal approach to measuring the critical shoulder angle (CSA) due to the malposition of the scapula. Three‐dimensional computed tomography (3D‐CT) may offer some advantages, including the ability to rotate the scapula for position alignment and pre‐operative planning for reducing CSA. This study aimed to investigate the accuracy and reliability of CSA measurement in 3D‐CT and to determine whether there is an association between CSA and rotator cuff tears (RCTs). Methods In this retrospective study we identified 200 patients who received shoulder arthroscopy from 2019 to 2021, including 142 patients (81 females, 61 males) with RCTs and 58 patients (14 females, 44 males) with non‐RCTs. For each participant, CSA was measured from standard shoulder AP radiographs and anterior views of 3D‐CT of the scapula by two independent assessors. Inter‐ and intra‐observer agreements were assessed by the intraclass correlation coefficient (ICC). The relationship between the two measurement methodologies was determined by Spearman's correlation coefficient and Bland–Altman plots. Discriminative capacity was calculated by using receiver operating curve (ROC) analyses in the whole cohort and age sub‐groups above and below 45 years. Results We found perfect inter‐observer (ICC >0.96) and intra‐observer (ICC >0.97) reliabilities for CSA measurements obtained from the standard AP radiographs and the 3D‐CT. There was a strong correlation between the two methods (r = 0.960, P < 0.001). The mean CSA was 31.7° ± 4.2° in the standard AP radiographs and 31.8° ± 4.4° in the 3D‐CT (mean difference 0.02°, P = 0.940; bias 0.02°, limits of agreement −2.29° to +2.33°). ROC analysis of the whole cohort showed that the CSA measured in the standard AP radiographs (area under the ROC curve [AUC] = 0.812, P < 0.001) and the 3D‐CT (AUC = 0.815, P < 0.001) predicted RCT with high confidence. ROC analysis of patients aged ≥45 years showed that the CSA measured from the standard AP radiographs (AUC = 0.869, P < 0.001) and the 3D‐CT (AUC = 0.870, P < 0.001) were very good at predicting RCTs. Conclusion CSA measured from standard AP radiographs and 3D‐CT showed high consistency, and the CSA could be accurately and reliably measured using 3D‐CT. CSAs measured from standard AP radiographs and 3D‐CT could predict RCTs, especially in patients aged ≥45 years