The complete chloroplast genome sequence of Sibiraea angustata, a traditional Chinese medicine in Sichuan Province, China

The complete chloroplast genome of Sibiraea angustata was assembled and subjected to phylogenetic analysis. The chloroplast genome of S. angustata was 155,869 bp in length, containing a large single-copy region (84,343 bp), a small single-copy region (18,820 bp), and two inverted repeat regions (26,353 bp). The overall GC content of S. angustata chloroplast genome was 36.80%. The chloroplast genome of S. angustata contained 127 unique genes, including 83 protein-coding genes, 36 tRNA genes and eight rRNA genes. Phylogenetic analysis revealed that S. angustata was related to Malus ioensis, Malus florentina and Malus trilobata

The complete chloroplast genome of Salix cupularis Rehder, a sand binder in alpine hillslope, China

The complete chloroplast genome of Salix cupularis Rehder was assembled and subjected to phylogenetic analysis. The chloroplast genome of S. cupularis was 155,518 bp in length, containing a large single-copy region (84,373 bp), a small single-copy region (16,226 bp), and two inverted repeat regions (27,458 bp). The overall GC content of S. cupularis chloroplast genome was 36.70%. The chloroplast genome of S. cupularis contained 127 unique genes, including 82 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis showed that S. cupularis was most related to Salix magnifica

Potential impact of climatic factors on the distribution of Graphium sarpedon in China

Abstract Graphium sarpedon is a significant foliar pest of Laurel plants in China. In this study, the MaxEnt model was used to investigate the distribution of G. sarpedon and predict its potential distribution areas in China in the future (2050s and 2090s) based on three Shared Socioeconomic Pathways (SSP1‐2.6, SSP2‐4.5, and SSP5‐8.5), and key environmental variables affecting its distribution were identified. The results showed that under the current climatic conditions, the suitable distribution areas of G. sarpedon were 92.17°–134.96° E and 18.04°–33.61° N, including Yangtze Plain (Middle and Lower), Pearl River Delta, Yangtze River Delta, and Lingnan areas. Under the future climate conditions, the total suitable distribution area of G. sarpedon decreased, but the area of medium suitable area increased. The study identified 11 key environmental variables affecting the distribution of G. sarpedon, the most critical of which was Precipitation of Warmest Quarter (bio18) and precipitation in April, May, June, and September (prec4, prec5, prec6, and prec9). This study is beneficial for monitoring and preventing the possible changes of G. sarpedon and provides theoretical references for its prevention and control

Characterization of the complete mitochondrial genome of the longhorn beetle, Batocera horsfieldi (Coleoptera, Cerambycidae) and its phylogenetic analysis with suitable longhorn beetles

Mitochondrial genome analysis is an important tool for studying insect phylogenetics. The longhorn beetle, Batocera horsfieldi, is a significant pest in timber, economic and protection forests. This study determined the mitochondrial genome of B. horsfieldi and compared it with the mitochondrial genomes of other Cerambycidae with the aim of exploring the phylogenetic status of the pest and the evolutionary relationships among some Cerambycidae subgroups. The complete mitochondrial genome of B. horsfieldi was sequenced by the Illumina HiSeq platform. The mitochondrial genome was aligned and compared with the existing mitochondrial genomes of Batocera lineolata and B. rubus in GenBank (MF521888, MW629558, OM161963, respectively). The secondary structure of transfer RNA (tRNA) was predicted using tRNAScan-SE server v.1.21 and MITOS WebSever. Thirteen protein-coding genes (PCGs) and two ribosomal RNA gene sequences of 21 longhorn beetles, including B. horsfieldi, plus two outgroups, Dryops ernesti (Dryopidae) and Heterocerus parallelus (Heteroceridae), were analyzed. The phylogenetic tree was constructed using maximum likelihood and Bayesian inference methods. In this study, we successfully obtained the complete mitochondrial genome of B. horsfieldi for the first time, which is 15 425 bp in length. It contains 37 genes and an A + T-rich region, arranged in the same order as the recognized ancestor of longhorn beetles. The genome of B. horsfieldi is composed of 33.12% A bases, 41.64% T bases, 12.08% C bases, and 13.16% G bases. The structure, nucleotide composition, and codon usage of the new mitochondrial genome are not significantly different from other longhorn mitochondrial genomes. Phylogenetic analyses revealed that Cerambycidae formed a highly supported single clade, and Vesperidae was either clustered with Cerambycidae or formed a separate clade. Interestingly, B. horsfieldi, B. rubus and B. lineolata were clustered with Monochamus and Anoplophora species in both analyses, with high node support. Additionally, the Vesperidae Spiniphilus spinicornis and Vesperus sanzi and the 19 Cerambycidae species formed a sister clade in the Bayesian analysis. Our results have produced new complete mitogenomic data, which will provide information for future phylogenetic and taxonomic research, and provide a foundation for future relevant research

The complete mitochondrial genome of Poecilocoris druraei (Linnaeus, 1771) (Hemiptera: Scutelleridae) and its phylogenetic implications

The complete mitochondrial genome sequence of Poecilocoris druraei was sequenced and analyzed. The 16,524 bp mitogenome of P. druraei contained 21 tRNA genes, two rRNA genes, 13 protein-coding genes (PCGs), and a control region. Most PCGs used standard ATG start codons and complete TAA ermination codons. Phylogenetic analysis indicated that P. druraei is closed related with P. nepalensis

Expression levels of the <i>B</i>. <i>horsfieldi</i> unigenes by FPKM analysis.

<p>(A) The perspective of general distribution. (B) The dispersing perspective. BF: <i>B</i>. <i>horsfieldi</i> females, BP: <i>B</i>. <i>horsfieldi</i> pupae, BL: <i>B</i>. <i>horsfieldi</i> larvae, BM: <i>B</i>. <i>horsfieldi</i> males.</p

Unigenes annotated in different databases.

<p>Unigenes annotated in different databases.</p

Differentially expressed OBPs between males and females.

<p>Differentially expressed OBPs between males and females.</p

Venn diagram of the number of differentially expressed genes in males, females, larvae, and pupae.

<p>BF: <i>B</i>. <i>horsfieldi</i> females, BP: <i>B</i>. <i>horsfieldi</i> pupae, BL: <i>B</i>. <i>horsfieldi</i> larvae, BM: <i>B</i>. <i>horsfieldi</i> males.</p

Scattergram of simple sequence repeats (SSRs) detected in the <i>B</i>. <i>horsfieldi</i> unigene sequences.

<p>Scattergram of simple sequence repeats (SSRs) detected in the <i>B</i>. <i>horsfieldi</i> unigene sequences.</p