{μ-N,N,N′,N′-Tetra­kis[(diphenyl­phosphan­yl)meth­yl]benzene-1,4-diamine}­bis­[(2,2′-bipyrid­yl)copper(I)] bis­(tetra­fluoridoborate)

In the title compound, [Cu2(C10H8N2)2(C58H52N2P4)](BF4)2, the dinuclear cation lies on an inversion centre. The CuI atom is coordinated by two N atoms from a 2,2′-bipyridine ligand and two P atoms from an N,N,N′,N′-tetra­kis­[(diphenyl­phos­phan­yl)meth­yl]benzene-1,4-diamine ligand in a distorted tetra­hedral geometry. In the crystal, inter­molecular C—H⋯F hydrogen bonds link the ions into layers parallel to [01]. π–π inter­actions [centroid–centroid distance = 3.668 (4) Å] are also observed. One F atom of the anion is disordered over two orientations with a refined occupancy ratio of 0.675 (13):0.325 (13)

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

BACKGROUND: MicroRNAs (miRNAs) are non-coding, single-stranded small RNAs that regulate gene expression negatively, which is involved in fundamental cellular processes. In this study, we investigated the role of miR-490-3P in the development of drug resistance in ovarian cancer cells. METHODS: The human ovarian carcinoma cell line A2780 and A2780/Taxol were exposed to paclitaxel in the presence or absence of microRNA 490-3P transfection, after which cell viability were performed by CCK-8 assay. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to assess the mRNA and protein expression levels of GST-π, MDR1 or P-gp. RESULTS: Our results showed higher miR-490-3P mRNA expression level in A2780/Taxol cells than in A2780 cells (p < 0.05). Following miR-490-3P transfection, both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel. The mRNA expression levels of MDR1, GST-π (p < 0.05) and protein expression levels of P-gp, GST-π were down-regulated after miR-490-3P transfection in comparison to mock and negative control cancer cells. CONCLUSION: Our results demonstrate for the first time that microRNA 490-3P may be involved in the development of drug resistance in ovarian cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13048-014-0084-4) contains supplementary material, which is available to authorized users

The evolution of floral deception in Epipactis veratrifolia (Orchidaceae): from indirect defense to pollination

BACKGROUND: It is estimated that floral deception has evolved in at least 7500 species of angiosperms, of which two thirds are orchids. Epipactis veratrifolia (Orchidaceae) is a model system of aphid mimicry as aphidophagous hoverflies lay eggs on false brood sites on their flowers. To understand the evolutionary ecology of floral deception, we investigated the pollination biology of E. veratrifolia across 10 populations in the Eastern Himalayas. We reconstructed the phylogeny of Epipactis and mapped the known pollination systems of previously studied species onto the tree. RESULTS: Some inflorescences of E. veratrifolia were so infested with aphids while they were still in bud that the some larvae of hoverflies developed to the third instar while flower buds opened. This indicated that adult female hoverflies were partly rewarded for oviposition. Although flowers failed to secrete nectar, they mimicked both alarm pheromones and aphid coloring of to attract female hoverflies as their exclusive pollinators. Phylogenetic mapping indicate that pollination by aphidophagous hoverflies is likely an ancestral condition in the genus Epipactis. We suggest that the biological interaction of aphid (prey), orchid (primary producer) and hoverfly (predator) may represent an intermediate stage between mutualism and deception in the evolution of pollination-by-deceit in E. veratrifolia. CONCLUSIONS: Our analyses indicate that this intermediate stage may be used as a model system to interpret the origin of oviposition (brood site) mimicry in Epipactis. We propose the hypothesis that some deceptive pollination systems evolved directly from earlier (partly) mutualistic systems that maintained the fidelity of the original pollinator(s) even though rewards (nectar/ brood site) were lost

Visualizing the dynamic behavior of poliovirus plus-strand RNA in living host cells

Dynamic analysis of viral nucleic acids in host cells is important for understanding virus–host interaction. By labeling endogenous RNA with molecular beacon, we have realized the direct visualization of viral nucleic acids in living host cells and have studied the dynamic behavior of poliovirus plus-strand RNA. Poliovirus plus-strand RNA was observed to display different distribution patterns in living Vero cells at different post-infection time points. Real-time imaging suggested that the translocation of poliovirus plus-strand RNA is a characteristic rearrangement process requiring intact microtubule network of host cells. Confocal-FRAP measurements showed that 49.4 ± 3.2% of the poliovirus plus-strand RNA molecules diffused freely (with a D-value of 9.6 ± 1.6 × 10(−10) cm(2)/s) within their distribution region, while the remaining (50.5 ± 2.9%) were almost immobile and moved very slowly only with change of the RNA distribution region. Under the electron microscope, it was found that virus-induced membrane rearrangement is microtubule-associated in poliovirus-infected Vero cells. These results reveal an entrapment and diffusion mechanism for the movement of poliovirus plus-strand RNA in living mammalian cells, and demonstrate that the mechanism is mainly associated with microtubules and virus-induced membrane structures

Model-Independent Determination of H0H_0 and ΩK,0\Omega_{K,0} using Time-Delay Galaxy Lenses and Gamma-Ray Bursts

Combining the `time-delay distance' ($D_{\Delta t}$) measurements from galaxy lenses and other distance indicators provides model-independent determinations of the Hubble constant ($H_0$) and spatial curvature ($\Omega_{K,0}$), only based on the validity of the Friedmann-Lema\^itre-Robertson-Walker (FLRW) metric and geometrical optics. To take the full merit of combining $D_{\Delta t}$ measurements in constraining $H_0$, we use gamma-ray burst (GRB) distances to extend the redshift coverage of lensing systems much higher than that of Type Ia Supernovae (SNe Ia) and even higher than quasars, whilst the general cosmography with a curvature component is implemented for the GRB distance parametrizations. Combining Lensing+GRB yields $H_0=71.5^{+4.4}_{-3.0}$~km s$^{-1}$Mpc$^{-1}$ and $\Omega_{K,0} = -0.07^{+0.13}_{-0.06}$ (1$\sigma$). A flat-universe prior gives slightly an improved $H_0 = 70.9^{+4.2}_{-2.9}$~km s$^{-1}$Mpc$^{-1}$. When combining Lensing+GRB+SN Ia, the error bar $\Delta H_0$ falls by 25\%, whereas $\Omega_{K,0}$ is not improved due to the degeneracy between SN Ia absolute magnitude, $M_B$, and $H_0$ along with the mismatch between the SN Ia and GRB Hubble diagrams at $z\gtrsim 1.4$. Future increment of GRB observations can help to moderately eliminate the $M_B-H_0$ degeneracy in SN Ia distances and ameliorate the restrictions on cosmographic parameters along with $\Omega_{K,0}$ when combining Lensing+SN Ia+GRB. We conclude that there is no evidence of significant deviation from a (an) flat (accelerating) universe and $H_0$ is currently determined at 3\% precision. The measurements show great potential to arbitrate the $H_0$ tension between the local distance ladder and cosmic microwave background measurements and provide a relevant consistency test of the FLRW metric.Comment: Accepted for publication in MNRA

A multi-angle hierarchical differential evolution approach for multimodal optimization problems

Multimodal optimization problem (MMOP) is one of the most common problems in engineering practices that requires multiple optimal solutions to be located simultaneously. An efficient algorithm for solving MMOPs should balance the diversity and convergence of the population, so that the global optimal solutions can be located as many as possible. However, most of existing algorithms are easy to be trapped into local peaks and cannot provide high-quality solutions. To better deal with MMOPs, considerations on the solution quality angle and the evolution stage angle are both taken into account in this paper and a multi-angle hierarchical differential evolution (MaHDE) algorithm is proposed. Firstly, a fitness hierarchical mutation (FHM) strategy is designed to balance the exploration and exploitation ability of different individuals. In the FHM strategy, the individuals are divided into two levels (i.e., low/high-level) according to their solution quality in the current niche. Then, the low/high-level individuals are applied to different guiding strategies. Secondly, a directed global search (DGS) strategy is introduced for the low-level individuals in the late evolution stage, which can improve the population diversity and provide these low-level individuals with the opportunity to re-search the global peaks. Thirdly, an elite local search (ELS) strategy is designed for the high-level individuals in the late evolution stage to refine their solution accuracy. Extensive experiments are developed to verify the performance of MaHDE on the widely used MMOPs test functions i.e., CEC’2013. Experimental results show that MaHDE generally outperforms the compared state-of-the-art multimodal algorithms

The topological differences between visitation and pollen transport networks: a comparison in species rich communities of the Himalaya–Hengduan Mountains

Pollination networks are usually constructed and assessed by direct field observations which commonly assume that all flower visitors are true pollinators. However, this assumption is often invalid and the use of data based on mere visitors to flowers may lead to a misunderstanding of intrinsic pollination networks. Here, using a large dataset by both sampling floral visitors and analyzing their pollen loads, we constructed 32 networks pairs (visitation versus pollen transport) across one flowering season at four elevation sites in the Himalaya–Hengduan Mountains region. Pollen analysis was conducted to determine which flower visitors acted as potential pollinators (pollen vectors) or as cheaters (those not carrying pollen of the visited plants). We tested whether there were topological differences between visitation and pollen transport networks and whether different taxonomic groups of insect visitors differed in their ability to carry pollen of the visited plants. Our results indicated that there was a significantly higher degree of specialization at both the network and species levels in the pollen transport networks in contrast to the visitation networks. Modularity was lower but nestedness was higher in the visitation networks compared to the pollen transport networks. All the cheaters were identified as peripheral species and most of them contributed positively to the nested structure. This may explain in part the differences in modularity and nestedness between the two network types. Bees carried the highest proportion of pollen of the visited plants. This was followed by Coleoptera, other Hymenoptera and Diptera. Lepidoptera carried the lowest proportion of pollen of the visited plants. Our study shows that the construction of pollen transport networks could provide a more in-depth understanding of plant–pollinator interactions. Moreover, it suggests that detecting and removing cheater interactions when studying the topology of other mutualistic networks might be also important.This study was supported by Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31020000), National Key Basic Research Program of China (2014CB954100), Joint Fund of the National Natural Science Foundation of China-Yunnan Province (U1502261), Major International Joint Research Project of NSF China (31320103919), Applied Fundamental Research Foundation of Yunnan Province (2014GA003), National Natural Science Foundation of China (31700361), Yunlin Scholarship of Yunnan Province to H. Wang (YLXL20170001) and CAS ‘Light of West China’ Program to Y.H. Zhao. A. Lázaro was supported by a Ramóny Cajal contract financed by the Spanish Ministry of Economy and Competitiveness