18 research outputs found
Preservasi, Konservasi dan Renovasi Kawasan Kota Tua Jakarta
“Great nation is a nation who\u27s always appreciates their own history,” that was a statement from Bung Karno. This paper is trying to lift a heritage district in Kota Tua Jakarta. A legacy that full of arts, cultures, stories, romance and tragedy that happened, and how the origin of the city formed. It\u27s very unfortunate if you see the condition right now. When all of the nations soo proud of their culture and history, everyone is competing to maintain and conserve their heritage and run the management very well. What happened with our heritage? Nowadays, Kota Tua district has been revitalized, but sadly, the process didn\u27t maintained well. So the results looks neglected and not in the good shape
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Verrucaria indet.
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Ag<sub>2</sub>Se Quantum Dots with Tunable Emission in the Second Near-Infrared Window
Quantum dots (QDs) with fluorescence in the second near-infrared
window (NIR-II, 1000–1400 nm) are ideal fluorophores for in
vivo imaging of deep tissue with high signal-to-noise ratios. Ag<sub>2</sub>Se (bulk band gap 0.15 eV) is a promising candidate for preparing
NIR-II QDs. By using 1-octanethiol as ligand to effectively balance
the nucleation and growth, tuning the fluorescence of Ag<sub>2</sub>Se QDs was successfully realized in the NIR-II window ranged from
1080 to 1330 nm. The prepared Ag<sub>2</sub>Se QDs can be conveniently
transferred to the aqueous phase by ligand exchange, showing great
potential for multicolor NIR-II fluorescence imaging in vivo
Myosin-Driven Intercellular Transportation of Wheat Germ Agglutinin Mediated by Membrane Nanotubes between Human Lung Cancer Cells
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for intercellular transfer of cellular contents. However, the transport mechanisms of membrane nanotubes remain poorly understood between cancer cells. Also largely unknown is the transport pattern mediated by membrane nanotubes. In this work, wheat germ agglutinin (WGA), a widely used drug carrier and potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as a model for exploring the intercellular transportation <i>via</i> membrane nanotubes. We found that membrane nanotubes allowed effective transfer of QDs-WGA. Long-term single-particle tracking indicated that the movements of QDs-WGA exhibited a slow and directed motion pattern in nanotubes. Significantly, the transport of QDs-WGA was driven by myosin molecular motors in an active and unidirectional manner. These results contribute to a better understanding of cell-to-cell communication for cancer research
Dissecting the Factors Affecting the Fluorescence Stability of Quantum Dots in Live Cells
Labeling
and imaging of live cells with quantum dots (QDs) has attracted great
attention in the biomedical field over the past two decades. Maintenance
of the fluorescence of QDs in a biological environment is crucial
for performing long-term cell tracking to investigate the proliferation
and functional evolution of cells. The cell-penetrating peptide transactivator
of transcription (TAT) is a well-studied peptide to efficiently enhance
the transmembrane delivery. Here, we used TAT peptide-conjugated QDs
(TAT–QDs) as a model system to examine the fluorescence stability
of QDs in live cells. By confocal microscopy, we found that TAT–QDs
were internalized into cells by endocytosis, and transported into
the cytoplasm via the mitochondria, Golgi apparatus, and lysosomes.
More importantly, the fluorescence of TAT–QDs in live cells
was decreased mainly by cell proliferation, and the low pH value in
the lysosomes could also lower the fluorescence intensity of intracellular
QDs. Quantitative analysis of the amount of QDs in the extracellular
region and whole cells indicated that the exocytosis was not the primary
cause of fluorescence decay of intracellular QDs. This work facilitates
a better understanding of the fluorescence stability of QDs for cell
imaging and long-term tracking in live cells. Also, it provides insights
into the utility of TAT for transmembrane transportation, and the
preparation and modification of QDs for cell imaging and tracking