Figure

Figur

The Emean, Emax, Emin and SD comparison in the central area of torsional testis measured by two senior sonographers.

<p>The Emean, Emax, Emin and SD comparison in the central area of torsional testis measured by two senior sonographers.</p

The Emean, Emax, Emin and SD comparison in the border area of torsional and normal testis.

<p>Compared with the Emean, Emax, Emin and SD values in the border area of torsional testis, *P<0.05.</p

The pathological result of torsional testis.

<p>6A-6B: the orchiectomy specimens showed that the size of torsional testis increased and the testis infarction appeared dark red or jet black; 6C-6E: HE staining showed that the lobular gap cavities of infarct testis was filled with diffuse hemorrhage and sporadic died interstitial cells, a large number of spermatogonia, extensive coagulation necrosis of spermatocytes in seminiferous tubules, interstitial hyperplasia and lymphocyte infiltration.</p

The SWE imaging of twisted and normal testicles.

<p>A: the SWE imaging of twisted testicles; B: the SWE imaging of normal testicles.</p

Effects of shPHD2 delivery on PHD2, HIF-1a, VEGF, bFGF and TGF-β1 mRNA and protein expression in H9C2 cells.

<p>A-E: Expression of PHD2, HIF-1,VEGF, TGF-β1and bFGF was determined by quantitative polymerase chain reaction(qPCR) in H9C2 cells of Normoxic & EGFP, Normoxic & shPHD2-EGFP, Hypoxic & EGFP and Hypoxic & shPHD2-EGFP group presented in the bar graph (asterisk indicates <i>P</i>-value<0.05).</p

The effect of ET on cell transfection efficiency and cell viability.

<p>(A): transfection efficiency at different exposure time; (B): cell viability at different exposure time. Indication: Symbols indicate significant differences (<i>P</i>< 0.05) from ET of 15 s (*) or 30 s (†).</p

Effects of shPHD2 delivery on PHD2, HIF-1a,VEGF, bFGF and TGF-β1 protein expression in H9C2 cells.

<p>A1, B1, C1 and D1: Representative Western-blot, showing bands of PHD2, HIF-1α,VEGF, bFGF and TGF-β1 in myocardial specimens collected from the indicated groups (asterisk indicates <i>P</i>—value<0.05). A2-A3,B2-B3,C2-C4 and D2-D4:Densitometric measurements of the PHD2, HIF-1α, VEGF, bFGF and TGF-β1 to GAPDH and presented in the bar graph (asterisk indicates <i>P</i>-value<0.05). Indication: Normoxic & EGFP: normoxic H9C2 cells transfected with EGFP plasmid; Normoxic & shPHD2-EGFP: normoxic H9C2 cells transfected with shPHD2-EGFP plasmid; Hypoxic & EGFP: hypoxic H9C2 cells transfected with EGFP plasmid; Hypoxic & shPHD2-EGFP: hypoxic H9C2 cells transfected with shPHD2-EGFP plasmid.</p

Effects of AI and MC on cell transfection efficiency and cell viability.

<p>(A1-A2): transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 0.5W/cm². (B1-B2): transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 1.0 W/cm². (C1-C2): transfection efficiency and cell viability at different MC(100 μl/ml, 200μl/ml, 300μl/ml and 400μl/ml) when AI was 1.5W/cm². (D1-D2) transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 2.0 W/cm². Indication: Symbols indicate significant differences (<i>P</i> < 0.05) from the 100μg/ml. (*), from 200μg/ml (†) and from 300μg/ml (‡)respectively.</p

The effect of PC on transfection efficiency and cell viability.

<p>A-D showing EGFP expression at PC 5 μg/ml,10 μg/ml,15 μg/ml, 20 μg/ml in sequence. E showing transfection efficiency at different PC with when MC (30%), AI (1.5 W/cm²), and ET(30 s) were fixed. F cell viability at different PC with when MC (30%), AI (1.5 W/cm²), and ET (30 s) were fixed. Indication: Symbols indicate significant differences (<i>P</i>< 0.05) from ET of 5 μg/ml(*) or 10μg/ml (†).</p