Electrical treeing breakdown properties for polyimide nanocomposite film in inverter-fed motors

The failure of magnet wires used in inter-turn insulation of inverter-fed motor winding is the main cause which leads to the motor insulation breakdown. This paper conducted electrical tree breakdown experiments on magnet wire insulating film (FCR 100) used in the inter-turn insulation of JD117 inverter-fed motor and meanwhile combining with energy spectrum analysis and scanning electrical microscope (SEM), by which type and content of elements, breakdown main body and electrical tree channel could be clearly observed, the corresponding failure mechanisms were analyzed. The experiment results reveal that all the breakdown main bodies are located at the pointedness and insulation joint of magnet wires, of which dielectric strength is relatively lower; they are the insulation "weak point", which should be paid enough attention and strengthened at the manufacturing period. The electrical tree breakdown time is quite long and the life span of magnet wire is 11 hours. The electrical breakdown strength of envelope is very low when containing metal impurity. It is also found that the aluminum occupies the highest content tested by energy spectrum analysis

Optimization of Load Distribution Method for Hydropower Units Based on Output Fluctuation Constraint and Double-Layer Nested Model

During the load distribution of hydropower units, the frequent crossing of vibration zones as well as large output fluctuations affect the stability of the power station. A multi-objective double-layer intelligent nesting model that considers the constraint of the output fluctuation of units is proposed to address these problems. The nonlinear constraint unit commitment optimization model layer is built based on outer dynamic programming, and the load distribution optimization model layer is constructed based on the improved biogeography-based optimization algorithm. Simultaneously, the unit output fluctuation constraint is established based on whether the unit combination changes in order to limit the unit output fluctuation. The results of this model indicate that compared with traditional load allocation models, the application of the method proposed in this paper can reduce the fluctuation range of unit output by 85.01%. In addition, except for the inevitable vibration zone crossings during startup and shutdown processes, the unit does not cross the vibration zone during operation, which greatly improves the unit’s vibration isolation and optimization capabilities. The multi-objective double-layer intelligent nested model proposed in this paper has significant advantages in the field of load allocation for hydropower units. It effectively improves the stability and reliability of unit operation, and this method can be applied to practical load allocation processes. It is of great significance for the research on load allocation optimization of hydropower units

Genetic and biochemical characterization of genes involved in hyaluronic acid synthesis in Streptococcus zooepidemicus

The biosynthetic pathway for hyaluronic acid (HA) has been proposed; however, a thorough genetic and functional analysis is required to further elucidate the roles of genes involved in HA production. Previously, we developed a markerless gene-deletion system for Streptococcus zooepidemicus and confirmed that hasA is essential for HA synthesis. Here, we constructed a comprehensive set of deletion mutants and investigated the roles of ten additional predicted genes in the HA synthetic pathway. Phenotypic assays revealed that all ten genes play a role in cell growth and/or HA synthesis. As expected, the deletion of hasA or hasB abolished HA production with little effect on growth, while the deletion of genes that are also required for peptidoglycan biosynthesis (hasE, glmM, and glmS) significantly reduced cell growth and HA production. Either of the glmU homologues (hasD and gcaD) was sufficient for optimal growth and the mucoid phenotype, while no double mutant could be isolated. Of the two UDP-glucose pyrophosphorylase (UGPase) paralogues, the operon-encoded hasC1 was responsible for 65 % of the activity, while hasC2 was responsible for the remaining 35 %. The deletion of hasC1 had no effect on cell growth and caused only a moderate decrease in the UDP-glucose level and HA production. The deletion of both hasC1 and hasC2 resulted in a severe growth defect and negligible UDP-glucose accumulation, HA production, and pyrophosphorylase activity. Of the two phosphoglucomutase paralogues, pgm1 and pgm2, the former is responsible for around 10 % of activity, while the latter is responsible for 90 %. The deletion of pgm1 showed no apparent effect on HA synthesis and growth, while the deletion of pgm2 resulted in the abolishment of HA synthesis and a significantly slower growth. These results should guide the metabolic engineering of S. zooepidemicus to improve HA productivity and quality

Deletion of RBPJK in Mesenchymal Stem Cells Enhances Osteogenic Activity by Up-Regulation of BMP Signaling.

Recently we have demonstrated the importance of RBPjk-dependent Notch signaling in the regulation of mesenchymal stem cell (MSC) differentiation during skeletogenesis both in vivo and in vitro. Here we further performed RBPJK loss-of-function experiments to demonstrate for the first time that RBPJK deficient MSC shows enhanced differentiation and osteogenesis acts via up-regulation of the BMP signaling. In the present study, we first compared the spontaneous and osteogenic differentiation in normal and recombination signal binding protein for immunoglobulin kappa J region (RBPJK) deficient human bone marrow-derived mesenchymal stem cells (MSCs). It was found that RBPJK highly expressed in fresh isolated MSCs and its expression was progressing down-regulated during spontaneous differentiation and even greater in osteogenic media inducted differentiation. Deletion of RBPJK in MSCs not only enhances cell spontaneous differentiation, but also significantly accelerates condition media inducted osteogenic differentiation by showing enhanced alkaline phosphatase (ALP) activity, Alizarin red staining, gene expression of Runx2, Osteopontin (OPN), Type I collagen (COL1a1) in culture. Additionally, BMP signaling responsive reporter activity and phosphor-smad1/5/8 expression were also significantly increased upon removal of RBPJK in MSCs. These data proved that inhibition of Notch signaling in MSCs promotes cell osteogenic differentiation by up-regulation of BMP signaling, and RBPJK deficient MSC maybe a better cell population for cell-based bone tissue engineering

Black Phosphorus: Degradation Favors Lubrication

Due to its innate instability, the degradation of black phosphorus (BP) with oxygen and moisture was considered the obstacle for its application in ambient conditions. Here, a friction force reduced by about 50% at the degraded area of the BP nanosheets was expressly observed using atomic force microscopy due to the produced phosphorus oxides during degradation. Energy-dispersive spectrometer mapping analyses corroborated the localized concentration of oxygen on the degraded BP flake surface where friction reduction was observed. Water absorption was discovered to be essential for the degraded characteristic as well as the friction reduction behavior of BP sheets. The combination of water molecules as well as the resulting chemical groups (P–OH bonds) that are formed on the oxidized surface may account for the friction reduction of degraded BP flakes. It is indicated that, besides its layered structure, the ambient degradation of BP significantly favors its lubrication behavior

Study on the Role of Salicylic Acid in Watermelon-Resistant Fusarium Wilt under Different Growth Conditions

Background: Fusarium wilt disease is leading threat to watermelon yield and quality. Different cultivation cropping systems have been reported as safe and efficient methods to control watermelon Fusarium wilt. However, the role of salicylic acid (SA) in watermelon resistance to Fusarium wilt in these different cultivation systems remains unknown. Methods: in this experiment, we used RNA-seq and qRT-PCR to study the effect of SA biosynthesis on improving watermelon health, demonstrating how it may be responsible for Fusarium wilt resistance under continuous monocropping and oilseed rape rotation systems. Results: the results revealed that the expression of the CIPALs genes was key to SA accumulation in watermelon roots. We observed that the NPR family genes may play different roles in responding to the SA signal. Differentially expressed NPRs and WRKYs may interact with other phytohormones, leading to the amelioration of watermelon Fusarium wilt. Conclusions: further understanding of gene expression patterns will pave the way for interventions that effectively control the disease

Formation Mechanism of Heavy Hydrocarbon Carbon Isotope Anomalies in Natural Gas from Ordovician Marine Carbonate in the Ordos Basin

Interactive depositional systems of marine carbonates and gypsum salt rocks are closely related to natural gas reservoirs. Despite continuous progress in the exploration of new areas of marine carbonate genesis within the Ordos Basin, the source and mechanism of “sub-salt” natural gas genesis remains controversial. In this study, we investigated natural gas genesis through geochemical analysis of Lower Paleozoic natural gas samples from the mid-eastern Ordos Basin, obtaining natural gas composition data and carbon/hydrogen isotope compositions. We found evident differences between the geochemical characteristics of “sub-salt” and “post-salt” natural gas; the methane carbon isotope signature of “sub-salt” natural gas was lighter overall than that of “post-salt” natural gas, while the ethane carbon isotope composition of the former was more widely distributed and partially lighter than that of the latter. Combining these data with the regional geological background and existing geochemical data, it is evident that Ordovician “post-salt” natural gas comprises a composite of Upper Paleozoic coal-type gas and Lower Paleozoic oil-type gas, with the oil-type gas accounting for the largest proportion. In contrast, the “sub-salt” natural gas was formed and preserved within the Ordovician marine carbonates or sourced from deeper and more ancient hydrocarbon source rocks. Geochemical anomalies, including light methane carbon isotopes and ethane carbon isotopes with coal-type gas characteristics, are closely related to the prevalence of thermochemical sulfate reduction during hydrocarbon formation and reservoir formation of natural gas in “sub-salt” strata

Enhanced BMP signaling in RBPJK deficient MSCs.

<p>(A) Real time RT-PCR analysis reveals that expression of RBPJK was significantly inhibited by 90% in shRBPJK lentivirus infected MSCs (shRBPJK) at day 2 when compared to shRNA-lentivirus control MSCs (Co). (B) Luciferase assays showed a significant increase of BMP responsive reporter activity in shRBPJK MSCs and this transcriptional up-regulation is further enhanced by BMP-2 treatment. Data are means ± s.d. of three independent experiments performed in duplicate and all the results were normalized to internal control (*, p < 0.05 compared with control MSCs (Co) without BMP-2 treatment). (C) Western Blot shows phosphor- smad1/5/8 (P-smad1/5/8) protein levels were significantly enhanced by deletion of RBPJK in MSC culture at day 2. β-actin was used as a loading control. (D) Quantification of P-smad1/5/8 protein level in Western blots was determined by measuring band intensity with ImageJ. (E) Western Blot shows deletion of RBPJK did not cause a change in phosphor-ERK1/2 (P-ERK1/2), total-ERK1/2 (T-ERK1/2), phosphor-p38 (P-p38) and total-p38 (T-p38) protein levels in MSC culture at day 2. (F) Densitometry quantification of activated P-ERK1/2 and P-p38, expressed as a ratio to total ERK and total p38 by P/T ratio. Data are means ± s.d. of three independent experiments. (*, p < 0.05 compared with shRNA-lentivirus control MSCs).</p