Preparing and characterizing Fe3O4@cellulose nanocomposites for effective isolation of cellulose-decomposing microorganisms

This study developed Fe3O4@cellulose nanocomposites by co-precipitation synthesis for bacteria capture and isolation. By surface modification with cellulose, the Fe3O4@cellulose nanocomposites have 20 nm average particle size and 3.3–24.9 emu/g saturation magnetization. Living bacteria could be captured by the Fe3O4@cellulose nanocomposites and harvested by magnetic field, with high efficiency (95.1%) and stability (>99.99%). By metabolizing cellulose and destroying the Fe3O4@cellulose@bacteria complex, cellulose-decomposing microorganisms lost the magnetism. They were therefore able to be isolated from the inert microbial community and the separation efficiency achieved over 99.2%. This research opened a door to cultivate the uncultivable cellulose-decomposing microorganisms in situ and further characterize their ecological functions in natural environment

Effect of Yuanbao Maple Tea Powder with High Chlorogenic Acid Content on Bread Quality

Using Yuanbao maple leaves as raw materials, the extraction process of chlorogenic acid in leaves was optimized, and single-factor and orthogonal experiments were carried out on ultrasonic temperature, time, and solid-liquid ratio through ultrasonic extraction. The results showed that the optimal level of the experiment was when the ratio of solid to liquid was 16:1, the concentration of ethanol was 60%, and the ultrasonic time was 15 min, and the extraction amount was 6.86% (mass fraction). Under the optimal extraction process conditions, the dynamic content of chlorogenic acid in the growth cycle of Yuanbaofeng in 2020 was analyzed. The results showed that the content of chlorogenic acid in the leaves of Yuanbaofeng in June was the highest, and the content in September was the least. In order to further explore the effect of Yuanbao maple tea powder on bread quality, different proportions of Yuanbao maple tea powder were added to bread to study its sensory effects on bread. The effects of scores, moisture content, texture, polyphenol content, antioxidant activity and other qualities. The results show that the water holding capacity, elasticity and anti-oxidation of bread are the best when the addition amount of GTB is 0.5%. Less elastic, more difficult to chew, and gradually unstable antioxidant properties

An Analysis on Comparison of the Static Load Test Performed to Post-Grouted Pile Foundation with Chinese, American and European Standard

In the paper, a study is conducted to pile foundation post-grouting and static load test, in which the tested value is used to compare with the standard of China, America and Europe. Taking Li-yuTuo bridge as study object, the trial piles - 3c2 and 3c4 with length of 33 meters and diameter of 1.2 meter are selected. The vertical bearing capacity of single pile is designed as 33000 kN which thereafter is enhanced using post-grouting. The on-site static load test is employed to evaluate the bearing capacity of pile foundation that is obtained being 39220 kN through self-balanced test. It is indicated through standard comparison that the bearing capacity calculated by China and America standard is akin to each other, turning to be excessively conservative and much less than the theoretical value of Europe. The Chinese standard shows bearing capacity after grouting is increased by 78.8 percent compared with pile foundation being not grouted. The post-grouting trial value is increased by 18.8 percent more than design value, 81.4 percent than theoretical value of general pile and 1.44 percent than theoretical value of post-grouting

LncRNA-ATB inhibits the proliferation and invasion of NSCLC cells by regulating MiR-200s expression

Purpose: To investigate the effect of lncRNA-ATB on the proliferation and invasion of non-small cell lung cancer (NSCLC) cells, and its mechanism of action. Methods: LncRNA-ATB mRNA levels in carcinoma tissues and normal adjacent tissues of 38 NSCLC patients in Peking University Shougang Hospital were determined by reverse transcription-polymerase chain reaction (RT-PCR). Human NSCLC A549 cell line was divided into control and lncRNA-ATB inhibition (si-ATB) groups, respectively. The proliferation and invasion of cells in each group were assessed. Subsequently, the effect of lncRNA-ATB inhibition on the growth of NSCLC cells was evaluated by subcutaneous tumor formation assay. Results: The expression of lncRNA-ATB was significantly higher in carcinoma tissues than in normal adjacent tissues in NSCLC patients. Cell counting kit-8 (CCK-8) assay results showed that si-ATB group displayed a weakened ability of the cells to proliferate (p < 0.05). Furthermore, deoxyribonucleic acid (DNA) replication ability was weaker in si-ATB group than in the control group. Wound healing assay results showed that the migration ability of cells in the si-ATB group was lower than that in the control group. Also, lncRNA-ATB knockdown inhibited the invasion ability of human NSCLC cells (p < 0.05). Tumor formation assay data indicate that lncRNA-ATB knockdown significantly repressed the subcutaneous tumor formation ability of NSCLC cells. Furthermore, lncRNA-ATB knockdown in NSCLC cells up-regulated miR-200a, miR-200b and miR-200c. Conclusion: The expression level of LncRNA-ATB is elevated in carcinoma tissues of NSCLC patients, and its knockdown suppresses the proliferation and invasion of NSCLC cells by up-regulating miR-200s. This finding suggests that it is a potential strategy for the management of NSCLC patients

Adaptive transmission in heterogeneous networks

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/166243/1/cmu2bf00018.pd