SacB-SacR Gene Cassette As the Negative Selection Marker to Suppress Agrobacterium Overgrowth in Agrobacterium-Mediated Plant Transformation

Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transfor-mation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. The antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. The SacB-SacR proteins are toxic to most Agrobacterium tumefaciens strains when they are grown on culture medium sup¬plemented with sucrose. Therefore, SacB-SacR genes can be used as negative selection markers to suppress the overgrowth of Agrobacterium tumefaciens in the plant tissue culture process. We generated a mutant Agrobacterium tumefaciens strain GV2260 (recA-SacB/R) that has the SacB-SacR cassette inserted into the bacterial genome at the recA gene locus. The mutant Agrobacterium strain is sensitive to sucrose but maintains its ability to transform plant cells in both transient and stable transformation assays. We demonstrated that the mutant strain GV2260 (recA-SacB/R) can be inhibited by sucrose that reduces the overgrowth of Agrobacterium and therefore improves the plant transformation efficiency. We employed GV2260 (recA-SacB/R) to generate stable transgenic N. benthamiana plants expressing a CRISPR-Cas9 for knocking out a WRKY transcrip¬tion factor

Radiologic comparison of posterior release, internal distraction, final PSO and spinal fusion with one-stage posterior vertebral column resection for multi-level severe congenital scoliosis

Abstract Background To compare radiologic results of posterior release, internal distraction, and final pedicle subtraction osteotomy (PSO) and spinal fusionwith one-stage posterior vertebral column resection (PVCR) in treating multi-level severe congenital scoliosis. Methods Forty-onesevere congenital scoliosis patients were used in the study. Group A comprised 24 patients who underwent one-stage PVCR. Group B comprised 17 patients who underwent posterior release with internal distraction, followed by final posterior fusion and instrumentation. The average preoperative main curve was 110.4° (95–130°) in group A and 109.4° (range 90°–126°) in group B. Postoperative follow-up time was ≥2 years (2.0–4.5 years) to analyze the radiographic and clinical outcomes. Results A comparison of posterior release, internal distraction, and final spinal fusion with PVCR showed no significant differences in postoperative main curve and compensatory caudal curve correction, coronal and sagittal imbalance. However, significant differences were found between the 2 groups in compensatory cranial curve correction. Conclusions Posterior release, internal distraction, and final spinal fusion produce better corrective results in compensatory cranial curve correction than PVCR in treating severe multi-level congenital scoliosis

Characterization and Electrochemical Behaviour of Ce(Ⅲ) Amino Acid Imidazole Ternary Complexes

合成了铈（ Ⅲ）＿色氨酸＿咪唑和铈（ Ⅲ）＿苯丙氨酸＿咪唑三元配合物，对配合物进行了元素分析、摩尔电导测试、热重＿差热分析及红外光谱分析，确定了配合物的组成和成键特征，并用循环伏安法研究了铈（ Ⅲ） 离子、铈（Ⅲ） 与氨基酸及咪唑混合溶液、铈（ Ⅲ） 二元及三元配合物在玻碳电极上的电化学行为．实验结果表明，在－ ０－１０ ～１－１０ Ｖ（ｖｓ．ＳＣＥ） 电位扫描范围内，两种铈（Ⅲ） 三元配合物显示了完全不可逆的电子迁移过程Two Ce(Ⅲ)_amino acid_imidazole teranry complexe were synthesized,These complexes have the composition of Ce(Trp) 3IM(NO 3) 3·3H 2O and Ce(Phe) 3IM(NO 3) 3 respectively. The IR spectra indicate that the carboxy group of amino_acid and the N atoms of imidazole are coordinated with Ce(Ⅲ) ion.Cyclic voltammetry was used to study the electrochemical behaviour of Ce(Ⅲ) ion,the mixture of Ce(Ⅲ) with ligands,and Ce(Ⅲ) binary or ternary complexes. Within the scan range of -0.1 to 1.10 V (vs.SCE),both of ternary complexes depicted completely irreversible electron transfer process.作者联系地址：上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234Author's Address: Dept.of Chem.,Shanghai Normal Univ.,Shanghai 20023

Interactions of miR395, 319 and 159 with Their Target Genes.

<p>Interactions of miR395, 319 and 159 (red circles) with their target genes (light blue circles and pink circles) are shown. Two targets are commonly regulated by miR319 and miR395 while seven targets are commonly regulated by miR319 and miR159.</p

Sequence Alignment of Inverted Repeats and a Neutral Invertase Gene.

<p>The central regions of the inverted repeat sequences 1 to 8, their homologous IR sequences from chromosomes 8, 2, 14 and 20 and a fragment of a neutral invertase gene (Glyma12g02690) were aligned. The miRNA and miRNA star sequences are highlighted in green and blue, respectively. Nucleotides that differ from the consensus sequence are shaded in red.</p

Electrochemical Behavior of Superoxide dismutase on Gold Minigrid with Thin Layer Cell

ＳＯＤ在薄层电池金微网栅电极上的电化学行为①吴霞琴＊刘倩许兵孟晓云章宗穰（上海师范大学化学系电化学研究室上海２００２３４）超氧化物歧化酶（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，简称ＳＯＤ）能专一消除生物氧化产物超氧阴离子（Ｏ－２），因而在抵御疾病，...The electrochemical behavior of superoxide dismutase (Cu, Zn SOD) was investigated on gold wire electrode and gold minigrid with thin layer cell by cyclic voltammetry. A quasi reversible redox wave of SOD has obtained on gold minigrid thin layer electrode, but only oxidation peak has observed on gold wire electrode. The electrochemical reaction parameters, such as the formal potential E 0 ′, diffusion coefficient D and standard rate constant k 0′ were estimated.作者联系地址：上海师范大学化学系电化学研究室Author's Address: Elec. Division Dept. of Chem., Shanghai Teacher′s Univ., Shanghai 20023