DNA Matrix Operation Based on the Mechanism of the DNAzyme Binding to Auxiliary Strands to Cleave the Substrate

Numerical computation is a focus of DNA computing, and matrix operations are among the most basic and frequently used operations in numerical computation. As an important computing tool, matrix operations are often used to deal with intensive computing tasks. During calculation, the speed and accuracy of matrix operations directly affect the performance of the entire computing system. Therefore, it is important to find a way to perform matrix calculations that can ensure the speed of calculations and improve the accuracy. This paper proposes a DNA matrix operation method based on the mechanism of the DNAzyme binding to auxiliary strands to cleave the substrate. In this mechanism, the DNAzyme binding substrate requires the connection of two auxiliary strands. Without any of the two auxiliary strands, the DNAzyme does not cleave the substrate. Based on this mechanism, the multiplication operation of two matrices is realized; the two types of auxiliary strands are used as elements of the two matrices, to participate in the operation, and then are combined with the DNAzyme to cut the substrate and output the result of the matrix operation. This research provides a new method of matrix operations and provides ideas for more complex computing systems

Psoralen and Bakuchiol Ameliorate M-CSF Plus RANKL-Induced Osteoclast Differentiation and Bone Resorption Via Inhibition of AKT and AP-1 Pathways in Vitro

Background/Aims: Psoralen and bakuchiol are the main active compounds found in the traditional Chinese medicine Psoralea corylifolia L., and have been used to treat osteoporosis. This study aims to investigate the anti-osteoporosis effects of these two compounds using osteoclasts precursor differentiation and bone absorption assays in vitro. Methods: Primary mouse osteoclasts precursor cells were induced by M-CSF (macrophage colony stimulating factor) plus RANKL (receptor activator of nuclear factor kappa-B ligand) in vitro. TRACP (tartrate-resistant acid phosphatase) enzyme activity and toluidine blue staining were used to observe the effects of psoralen and bakuchiol on osteoclast differentiation and bone resorption, respectively. Gelatin zymography was used to assess MMP (matrix metalloproteinase) activity, and ELISA was performed to measure cathepsin K activity. Western blotting analysis for expression of phosphorylated AKT, ERK, NF-kB, and c-jun; and immunofluorescence analysis for c-jun and p65 nuclear translocation in induced osteoclasts were then used to determine the mechanism of anti-bone resorption of psoralen and bakuchiol. Results: Mature osteoclasts were induced by M-CSF plus RANKL from primary bone marrow macrophages in vitro. Both psoralen and bakuchiol significantly inhibited TRACP enzyme activity and slightly decreased the number of TRACP+ multinuclear osteoclasts induced by M-CSF plus RANKL. Bakuchiol significantly decreased bone lacunae area and attenuated MMP-2 activity induced by M-CSF plus RANKL in osteoclasts. Both psoralen and bakuchiol significantly decreased the expression and nuclear translocation of phosphorylated c-jun stimulated by M-CSF plus RANKL, but no significant effect on p65 translocation was observed in osteoclasts. Additionally, bakuchiol significantly attenuated the increased of M-CSF plus RANKL-induced phosphorylation of AKT in osteoclasts. Conclusions: Psoralen and bakuchiol ameliorated M-CSF plus RANKL-induced osteoclast differentiation and bone resorption via inhibition of AKT and AP-1 pathways activation in vitro

Neuroprotective Effect of Xueshuantong for Injection (Lyophilized) in Transient and Permanent Rat Cerebral Ischemia Model

Xueshuantong for Injection (Lyophilized) (XST), a Chinese Materia Medica standardized product extracted from Panax notoginseng (Burk.), is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction clinically in China. In the present study, we evaluated the acute and extended protective effects of XST in different rat cerebral ischemic model and explored its effect on peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 signaling pathway. We found that XST treatment for 3 days could significantly inhibit transient middle cerebral artery occlusion (MCAO) induced infarct volume and swelling percent and regulate the mRNA expression of interleukin-1β (IL-1β), IL-17, IL-23p19, tumor necrosis factor-α (TNFα), and inducible nitric oxide synthase (iNOS) in brain. Further study demonstrated that treatment with XST suppressed the protein expression of peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 and phosphorylation level of p38 and upregulated the phosphorylation level of STAT3. In permanent MCAO rats, XST could reduce the infarct volume and swelling percent. Moreover, our results revealed that XST treatment could increase the rats’ weight and improve a batch of functional outcomes. In conclusion, the present data suggested that XST could protect against ischemia injury in transient and permanent MCAO rats, which might be related to Prx6-TLR4 pathway

Surface nanocrystallization of Cu-Cr alloy by a high power density continuous laser beam

A nanostructured surface layer of similar to 300 mu m thickness was fabricated on Cu-30Cr (wt%) hypereutectic alloy by a continuous laser beam with high power density (1.08 x 10(7) W/cm(2)). The average grain size of Cr-rich particles was refined to similar to 40 nm, and the solid solubility limit of Cr in Cu was extended to 1.96 at. %. Experimental results show that the dispersion of nano-sized Cr-rich spheroids in Cu-rich matrix was attributed to the Brownian motion of Cr-rich spheroids, and the high cooling rate (5.75 x 10(6) K/s) during liquid phase separation which inhibits the collisions between Cr-rich spheroids. (C) 2018 Published by Elsevier B.V

drivingmechanismofkeyholeevolutionduringmultipulsedrillingwithamillisecondlaser

To better understand the physical processes of multi-pulse laser drilling,this study investigates the keyhole evolution and its driving mechanism in a time-resolved observation system.The evolution characteristics suggested a two-phase process of rapid penetration followed by moderate penetration.As revealed in the ejection and vaporization behavior,the keyhole evolution was dominated by ejection and vaporization during the rapid and moderate penetration stages,respectively.In a single laser-pulsed drilling experiment,the driving mechanism itself was found to be affected by the dimensionless laser power density.The effect of dimensionless laser power density on depth increment was then discussed by comparing the experimental observations with numerical simulation results.The results further confirmed the driving mechanism of the keyhole evolution.The results in this paper are useful for understanding the driving mechanism of the keyhole evolution during multi-pulse laser drilling

K-Modulated Co Nanoparticles Trapped in La-Ga-O as Superior Catalysts for Higher Alcohols Synthesis from Syngas

Owing to the outstanding catalytic performance for higher alcohol synthesis, Ga-Co catalysts have attracted much attention. In view of their unsatisfactory stability and alcohol selectivity, herein, K-modulated Co nanoparticles trapped in La-Ga-O catalysts were prepared by the reduction of La1−xKxCo0.65Ga0.35O3 perovskite precursor. Benefiting from the atomic dispersion of all the elements in the precursor, during the reduction of La1−xKxCo0.65Ga0.35O3, Co nanoparticles could be confined into the K-modified La-Ga-O composite oxides, and the confinement of La-Ga-O could improve the anti-sintering performance of Co nanoparticles. In addition, the addition of K modulated parts of La-Ga-O into La2O3, which ameliorated the anti-carbon deposition performance. Finally, the addition of K increased the dispersion of cobalt and provided more electron donors to metallic Co, resulting in a high activity and superior selectivity to higher alcohols. Benefiting from the above characteristics, the catalyst possesses excellent activity, good selectivity, and superior stability

Driving mechanism of keyhole evolution during multi-pulse drilling with a millisecond laser

To better understand the physical processes of multi-pulse laser drilling, this study investigates the keyhole evolution and its driving mechanism in a time-resolved observation system. The evolution characteristics suggested a two-phase process of rapid penetration followed by moderate penetration. As revealed in the ejection and vaporization behavior, the keyhole evolution was dominated by ejection and vaporization during the rapid and moderate penetration stages, respectively. In a single laser-pulsed drilling experiment, the driving mechanism itself was found to be affected by the dimensionless laser power density. The effect of dimensionless laser power density on depth increment was then discussed by comparing the experimental observations with numerical simulation results. The results further confirmed the driving mechanism of the keyhole evolution. The results in this paper are useful for understanding the driving mechanism of the keyhole evolution during multi-pulse laser drilling

Grain Refinement of Hypereutectic Immiscible Cu-50Cr Alloy during Rapid Melting and Solidification Induced by High Power Density Laser Beams

The microstructure of hypereutectic immiscible Cu-50Cr alloy (wt%) was effectively refined and homogenized by a high power density Nd:YAG continuous laser beam (power density 104−105 MW/m2). The average grain size of Cr was effectively refined from ~100 μm to ~1 μm, and some of the Cr particles even decreased to a few hundred nanometers. The high cooling rate (7.29 × 106 K/s) effectively inhibited the coarsening effect on Cr particles during liquid phase separation (LPS). The spherical Cr particles were preferably dispersed in the melt layer, and the thickness of the layer was up to ~150 μm. The refinement and dispersion of the Cr phase contributed to improving the interruption capability of the Cu-Cr contacts. Compared with the untreated samples, the arc duration and the withstanding voltage of the laser surface melting (LSM) treated contacts with refined microstructure increased to 21% and 33%, respectively. The results demonstrated that the LSM method was an effective approach to optimize the microstructure of Cu-Cr alloy, which made it a promising modification method for Cu-Cr vacuum contact applications

Dynamic evolution of keyhole during multi-pulse drilling with a millisecond laser on 304 stainless steel

The efficiency of millisecond laser drilling is high due to the molten material ejection, and is applied in many industries like aerospace, automobile and electronics. However, there may be some defects such as hole blockage, low machining accuracy and low repeatability, especially for the process of multi-pulse drilling. The melt flow remaining inside keyhole due to inefficient ejection, is responsible for the formation of those defects. Hence, it is necessary to investigate the dynamic behavior of melt flow during multi-pulse drilling. In this paper, the dynamic evolution of keyhole is investigated combining in-situ observation and numerical simulation methods. According to the ejection efficiency, the multi-pulse drilling process can be divided into three periods, namely rapid drilling period, linear drilling period, and moderate drilling period. The melt flow behaves differently in each period. In rapid drilling period, a conical keyhole is formed in the end. The melt flow behavior is affected dominantly by the recoil pressure when the laser is on and by the surface tension when the laser is off. In linear drilling period, the blocked keyhole occurs occasionally. The melt flow behavior is affected by gravity and recoil pressure when the laser is on and by the surface tension and recoil pressure when the laser is off. In moderate drilling period, the keyhole profile is wavy. The melt flow behavior is affected by surface tension and recoil pressure dominantly during the whole period. The melt flow transition and its influence factors are investigated in this paper, which is helpful for understanding the physical processes during the multi-pulse laser irradiation process