Toward a systems-level view of dynamic phosphorylation networks

To better understand how cells sense and respond to their environment, it is important to understand the organization and regulation of the phosphorylation networks that underlie most cellular signal transduction pathways. These networks, which are composed of protein kinases, protein phosphatases and their respective cellular targets, are highly dynamic. Importantly, to achieve signaling specificity, phosphorylation networks must be regulated at several levels, including at the level of protein expression, substrate recognition, and spatiotemporal modulation of enzymatic activity. Here, we briefly summarize some of the traditional methods used to study the phosphorylation status of cellular proteins before focusing our attention on several recent technological advances, such as protein microarrays, quantitative mass spectrometry, and genetically-targetable fluorescent biosensors, that are offering new insights into the organization and regulation of cellular phosphorylation networks. Together, these approaches promise to lead to a systems-level view of dynamic phosphorylation networks

Analysis on Safety of Removing the Closure Segment in a Prestressed Concrete Cable-stayed Bridge

AbstractAiming at failure of closure segment in a prestressed concrete cable-stayed bridge, a strengthening technology, namely replacing the closure segment, was firstly put forward. But removing the old closure segment was a process of release of internal force and had great risk. So the structural safety possibly induced by removing must be analyzed and confirmed. Based on FEM and summary of engineering experience, the construction stages for removing the old closure segment were simulated, and then some analysis relevant to safety, including thermal effect, dynamic characteristics and global stability of the whole bridge structure, were systematically presented. According to these analysis results, corresponding prevention and control measures were provided to ensure construction safety. Studies showed that, variation range of its structural state between before and after removing is not obvious, and its dynamic characteristics changed little after removing. In addition, structural instability could not be induced by removing, but for the sake of improving construction safety reliability, necessary safety prevention and control measures were indispensable. Analysis on safety of removing the old closure segment constituted the important part of the strengthening technology of replacing the closure segment, and became the theoretical basis of removing partial structural members for existing bridges

Traffic Scheduling Strategy of Power Communication Network Based on SDN

Due to the complicated structure, power communication network is difficult to guarantee the quality of service (QoS) of power services. A two-level scheduling algorithm based on software defined network (SDN) is proposed in this paper. Firstly, the priority-based scheduling method is used to meet the latency-sensitive of power service. Then, in order to alleviate congestion, queue bandwidth is adjusted according to network state information, which can be collected by the centralized control of SDN. Finally, the Mininet and Ryu controller are made use of building simulation environment. The test results show that the algorithm proposed in this paper reduce delay and packet loss rate significantly, which achieves QoS

A Research on Students’ Needs for Follow-up Curriculum of College English

Increased universities and colleges offer the undergraduates with more follow-up courses with the further reform in college English education in China. An investigation on self-evaluation, difficulty, and willingness of undergraduates in learning English further was made in order to design more appropriate and adaptable follow-up courses. This research shows that the majority of undergraduates are not satisfied with their English level in English skills, especially speaking. Most undergraduates are willing to take further college English courses with the purpose of finding a better job, but they prefer to choose some comparatively simple and entertaining selective courses such as movie appreciation other than some courses for developing skills. The data achieved leaves some implications on college English education, lays some bases for English education reform and provides new challenge for college English teachers