Multi-Channel Lanthanide Nanocomposites for Customized Synergistic Treatment of Orthotopic Multi-Tumor Cases

<p>Simultaneous photothermal ablation of multiple tumors is limited by unpredictable photo-induced apoptosis, caused by individual intratumoral differences. Here, a multi-channel lanthanide nanocomposite was used to achieve tailored synergistic treatment of multiple subcutaneous orthotopic tumors under non-uniform whole-body infrared irradiation prescription. The nanocomposite reduces intratumoral glutathione by simultaneously activating the fluorescence and photothermal channels. The fluorescence provides individual information on different tumors, allowing customized prescriptions to be made. This enables optimal induction of hyperthermia and dosage of chemo drugs, to ensure treatment efficacy, while avoiding overtherapy. With an accessional therapeutic laser system, customized synergistic treatment of subcutaneous orthotopic cancer cases with multiple tumors is possible with both high efficacy and minimized side effects.</p&gt

Multi-Channel Lanthanide Nanocomposites for Customized Synergistic Treatment of Orthotopic Multi-Tumor Cases

Simultaneous photothermal ablation of multiple tumors is limited by unpredictable photo-induced apoptosis, caused by individual intratumoral differences. Here, a multi-channel lanthanide nanocomposite was used to achieve tailored synergistic treatment of multiple subcutaneous orthotopic tumors under non-uniform whole-body infrared irradiation prescription. The nanocomposite reduces intratumoral glutathione by simultaneously activating the fluorescence and photothermal channels. The fluorescence provides individual information on different tumors, allowing customized prescriptions to be made. This enables optimal induction of hyperthermia and dosage of chemo drugs, to ensure treatment efficacy, while avoiding overtherapy. With an accessional therapeutic laser system, customized synergistic treatment of subcutaneous orthotopic cancer cases with multiple tumors is possible with both high efficacy and minimized side effects.</p

Revisión sobre métodos de preparación, mecanismos y aplicaciones de péptidos antioxidantes en aceites

Natural antioxidants, especially those used in edible oil, are safer compared to chemically synthesized antioxidants. Therefore, research on natural antioxidants has become prevelant. Antioxidant peptides derived from food protein can effectively prevent oil oxidation. Protein hydrolyzation is widely applied for the production of antioxidant peptides in industry, and bioinformatics is employed nowadays to generate the desired peptide sequence. Furthermore, the mechanism of antioxidant peptides in the oil system is still controversial, which limits the further development of antioxidant peptides as food antioxidants. This review introduces the preparation method of antioxidant peptides and their mechanisms as well as applications in the oil. It will help to comprehensively understand the function of antioxidant peptides and promote their development in the oil field.Los antioxidantes naturales, especialmente utilizados en aceites comestibles, son más seguros en comparación con los antioxidantes sintetizados químicamente. Por lo tanto, la investigación sobre antioxidantes naturales se convierte en un punto de interés. Los péptidos antioxidantes derivados de las proteínas alimentarias pueden prevenir eficazmente la oxidación del aceite. La hidrolización de proteínas se usa ampliamente en la industria para la producción de péptidos antioxidantes y la bioinformática se emplea hoy en día para generar la secuencia de péptidos deseada. Además, el mecanismo de los péptidos antioxidantes en el sistema oleoso sigue siendo controvertido, lo que limita el desarrollo posterior de péptidos antioxidantes como antioxidantes alimentarios. Esta revisión presenta el método de preparación de péptidos antioxidantes y su mecanismo, así como las aplicaciones en aceite, lo que ayudará a comprender de manera integral la función de los péptidos antioxidantes y promoverá su desarrollo en el campo petrolero

Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics

Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost-efficiency, especially for point-of-care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo-/chemo-stability, which maintains an outstanding serodiagnostic performance (accuracy &gt;95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes

<i>Sneathiella chinensis</i> gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China

The taxonomic position of strain LMG 23452T, which was isolated from coastal sediment from an aquaculture site near Qingdao, China, in 2000, was determined. Strain LMG 23452T comprised Gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, chemoheterotroph that produces catalase and oxidase. Comparative 16S rRNA gene sequence analysis revealed that strain LMG 23452T shared approximately 89 % sequence similarity with members of the genera Devosia, Hyphomonas, Ensifer and Chelatococcus, which belong to two different orders within the Alphaproteobacteria. Further phylogenetic analysis of the 16S rRNA gene sequence showed that strain LMG 23452T formed a separate branch within the order Rhizobiales, falling between the genera Devosia and Ensifer of the families Hyphomicrobiaceae and Rhizobiaceae, respectively. Strain LMG 23452T could be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including hydrolysis of the substrates starch and casein and assimilation of the carbohydrates d-glucose, d-mannose, mannitol, maltose and l-arabinose, and chemotaxonomically by the presence of the fatty acids C14 : 0 3-OH, C16 : 1ω11c, C16 : 1 ω5c and C18 : 1ω5c. The major fatty acids detected in strain LMG 23452T were C18 : 1 ω7c, C16 : 0, C19 : 0 cyclo ω8c, C16 : 1 ω7c and C17 : 1ω6c and the G+C content of the genomic DNA was 57.1 mol%. Therefore, the polyphasic data support the placement of strain LMG 23452T within a novel genus and species, for which the name Sneathiella chinensis gen. nov., sp. nov. is proposed. The type strain is LMG 23452T (=CBMAI 737T)

Current and charge distributions of the fractional quantum Hall liquids with edges

An effective Chern-Simons theory for the quantum Hall states with edges is studied by treating the edge and bulk properties in a unified fashion. An exact steady-state solution is obtained for a half-plane geometry using the Wiener-Hopf method. For a Hall bar with finite width, it is proved that the charge and current distributions do not have a diverging singularity. It is shown that there exists only a single mode even for the hierarchical states, and the mode is not localized exponentially near the edges. Thus this result differs from the edge picture in which electrons are treated as strictly one dimensional chiral Luttinger liquids.Comment: 21 pages, REV TeX fil

Predicting knee osteoarthritis

Treatment options for osteoarthritis (OA) beyond pain relief or total knee replacement are very limited. Because of this, attention has shifted to identifying which factors increase the risk of OA in vulnerable populations in order to be able to give recommendations to delay disease onset or to slow disease progression. The gold standard is then to use principles of risk management, first to provide subject-specific estimates of risk and then to find ways of reducing that risk. Population studies of OA risk based on statistical associations do not provide such individually tailored information. Here we argue that mechanistic models of cartilage tissue maintenance and damage coupled to statistical models incorporating model uncertainty, united within the framework of structural reliability analysis, provide an avenue for bridging the disciplines of epidemiology, cell biology, genetics and biomechanics. Such models promise subject-specific OA risk assessment and personalized strategies for mitigating or even avoiding OA. We illustrate the proposed approach with a simple model of cartilage extracellular matrix synthesis and loss regulated by daily physical activity

Semileptonic BcB_c decays and Charmonium distribution amplitude

In this paper we study the semileptonic decays of the $B_c$ meson in the Light-Cone Sum Rule (LCSR) approach. The result for each channel depends on the corresponding distribution amplitude of the final meson. For the case of $B_c$ decaying into a pseudoscalar meson, to twist-3 accuracy only the leading twist distribution amplitude (DA) is involved if we start from a chiral current. If we choose a suitable chiral current in the vector meson case, the main twist-3 contributions are also eliminated and we can consider the leading twist contribution only. The leading twist distribution amplitudes of the charmonium and other heavy mesons are given by a model approach in the reasonable way. Employing this charmonium distribution amplitude we find the cross section $\sigma(e^+e^-\to J/\psi+\eta_c)\simeq22.8 {fb}$ which is consistent with Belle and BaBar's data. Based on this model, we calculate the form factors for various $B_c$ decay modes in the corresponding regions. Extrapolating the form factors to the whole kinetic regions, we get the decay widths and branching ratios for various $B_c$ decay modes including their $\tau$ modes when they are kinematically accessible.Comment: Changed content partially, Added references, 16 pages,2 figure

C8-substituted imidazotetrazine analogs overcome temozolomide resistance by inducing DNA adducts and DNA damage

Copyright © 2019 Yang, Wei, Dai, Stevens, Bradshaw, Luo and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Temozolomide (TMZ) is the standard of care chemotherapeutic agent used in the treatment of glioblastoma multiforme. Cytotoxic O6-methylguaine lesions formed by TMZ are repaired by O6-methyl-guanine DNA methyltransferase (MGMT), a DNA repair protein that removes alkyl groups located at the O6-position of guanine. Response to TMZ requires low MGMT expression and functional mismatch repair. Resistance to TMZ conferred by MGMT, and tolerance to O6-methylguanine lesions conferred by deficient MMR severely limit TMZ clinical applications. Therefore, development of new TMZ derivatives that can overcome TMZ-resistance is urgent. In this study, we investigated the anti-tumor mechanism of action of two novel TMZ analogs: C8-imidazolyl (377) and C8-methylimidazole (465) tetrazines. We found that analogs 377 and 465 display good anticancer activity against MGMT-overexpressing glioma T98G and MMR deficient colorectal carcinoma HCT116 cell lines with IC50 value of 62.50, 44.23, 33.09, and 25.37 μM, respectively. Analogs induce cell cycle arrest at G2/M, DNA double strand break damage and apoptosis irrespective of MGMT and MMR status. It was established that analog 377, similar to TMZ, is able to ring-open and hydrolyze under physiological conditions, and its intermediate product is more stable than MTIC. Moreover, DNA adducts of 377 with calf thymus DNA were identified: N7-methylguanine, O6-methylguanine, N3-methyladenine, N3-methylthymine, and N3-methylcytidine deoxynucleotides. We conclude that C8 analogs of TMZ share a mechanism of action similar to TMZ and are able to methylate DNA generating O6-methylguanine adducts, but unlike TMZ are able at least in part to thwart MGMT- and MMR-mediated resistance