High expression of nucleophosmin is closely related to the grade and invasion of colorectal cancer

420-426This study explores the differential protein expression in the colorectal cancer (CRC) patients to validate a new biomarker for tumor progression. CRC tissues and their adjacent non-cancerous tissues were analyzed by two-dimensional LC/MS/MS. Nucleophosmin 1 (NPM1) was selected and confirmed its differential expression by Western blot. Immunohistological staining of NPM1 in tissues was performed to validate its correlation with clinicopathologic parameters of CRC patients. There were 39 candidates with significant difference between cancerous tissues and their adjacent non-cancerous tissues, which included 19 increased proteins and 20 decreased proteins in CRC samples. Especially, NPM1 was correlated with poor differentiation, and lymph node metastasis according to the analysis of patients’ clinicopathologic parameters. Increased expression of NPM1 can be as a critical biomarker for clinical diagnosis of tumor progression of CRC patients

High expression of nucleophosmin is closely related to the grade and invasion of colorectal cancer

To explore the differential protein expression in the colorectal cancer (CRC) patients to validate a new biomarker for tumor progression. CRC tissues and their adjacent non-cancerous tissues were analyzed by two-dimensional LC/MS/MS. Nucleophosmin 1 (NPM1) was selected and confirmed its differential expression by Western blot. Immunohistological staining of NPM1 in tissues was performed to validate its correlation with clinicopathologic parameters of CRC patients. There were 39 candidates with significant difference between cancerous tissues and their adjacent non-cancerous tissues, which included 19 increased proteins and 20 decreased proteins in CRC samples. Especially, NPM1 was correlated with poor differentiation, and lymph node metastasis according to the analysis of patients’ clinicopathologic parameters. Increased expression of NPM1 can be as a critical biomarker for clinical diagnosis of tumor progression of CRC patients

Transcriptome and Metabolome Analyses Reveal Differences in Terpenoid and Flavonoid Biosynthesis in Cryptomeria fortunei Needles Across Different Seasons

Cryptomeria fortunei (Chinese cedar) has outstanding medicinal value due to its abundant flavonoid and terpenoid contents. The metabolite contents of C. fortunei needles differ across different seasons. However, the biosynthetic mechanism of these differentially synthesized metabolites (DSMs) is poorly understood. To improve our understanding of this process, we performed integrated non-targeted metabolomic liquid chromatography and gas chromatography mass spectrometry (LC-MS and GC-MS), and transcriptomic analyses of summer and winter needles. In winter, the C. fortunei needle ultrastructure was damaged, and the chlorophyll content and Fv/Fm were significantly (p < 0.05) reduced. Based on GC-MS and LC-MS, we obtained 106 and 413 DSMs, respectively; based on transcriptome analysis, we obtained a total of 41.17 Gb of clean data and assembled 33,063 unigenes, including 14,057 differentially expressed unigenes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DSMs/DEGs were significantly (p < 0.05) enriched in many biosynthesis pathways, such as terpenoids, photosynthates, and flavonoids. Integrated transcriptomic and metabonomic analyses showed that seasonal changes have the greatest impact on photosynthesis pathways, followed by terpenoid and flavonoid biosynthesis pathways. In summer Chinese cedar (SCC) needles, DXS, DXR, and ispH in the 2-methyl-pentaerythritol 4-phosphate (MEP) pathway and GGPS were highly expressed and promoted the accumulation of terpenoids, especially diterpenoids. In winter Chinese cedar (WCC) needles, 9 genes (HCT, CHS, CHI, F3H, F3'H, F3'5'H, FLS, DFR, and LAR) involved in flavonoid biosynthesis were highly expressed and promoted flavonoid accumulation. This study broadens our understanding of the metabolic and transcriptomic changes in C. fortunei needles caused by seasonal changes and provides a reference regarding the adaptive mechanisms of C. fortunei and the extraction of its metabolites

Wafer Surface Defect Detection Based on Feature Enhancement and Predicted Box Aggregation

For wafer surface defect detection, a new method based on improved Faster RCNN is proposed here to solve the problems of missing detection due to small objects and multiple boxes detection due to discontinuous objects. First, focusing on the problem of small objects missing detection, a feature enhancement module (FEM) based on dynamic convolution is proposed to extract high-frequency image features, enrich the semantic information of shallow feature maps, and improve detection performance for small-scale defects. Second, for the multiple boxes detection caused by discontinuous objects, a predicted box aggregation method is proposed to aggregate redundant predicted boxes and fine-tune real predicted boxes to further improve positioning accuracy. Experimental results show that the mean average precision of the proposed method, when validated on a self-developed dataset, reached 87.5%, and the detection speed was 0.26 s per image. The proposed method has a certain engineering application value

Normative Evaluation Method of Long Jump Action Based on Human Pose Estimation

Addressing the issue of the lack of objective quantitative evaluation in training long jump events, this study presents a normative analysis method based on human pose estimation and similarity measures. By training a lightweight human pose estimation model, this method can run on low-delay embedded devices. In line with key movements, the proposed method designs a normative analysis for long jump actions, which yields a measurement of the movements’ adherence to the standard and provides corrective suggestions. Experimental results indicate that the accuracy of this approach in analyzing the standardization of long jump action reaches 91.3%. As a result, it holds significant application value in various scenarios, including students’ long jump training and correction of long jump techniques. Furthermore, it can be extended to other practical applications beyond sports

Advances and prospects of drug clinical research in colorectal cancer in 2022

Abstract Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer death worldwide. Clinical research results have provided more treatment opportunities for CRC patients, showing that an optimal combination of existing drugs and new drugs is needed to mitigate the burden of this disease. In this review, we have summarized recent advances in drug clinical research for CRC in 2022, including chemotherapy, targeted therapy, and immunotherapy, to find opportunities for substantial improvements in drug discovery and clinical development methods

Significantly Suppressed Dielectric Loss and Enhanced Breakdown Strength in Core@Shell Structured Ni@TiO₂/PVDF Composites

An insulating shell on the surface of conductive particles is vital for restraining the dielectric loss and leakage current of polymer composites. So as to inhibit the enormous loss and conductivity of pristine nickel (Ni)/poly(vinylidene fluoride)(PVDF) composites but still harvest a high dielectric permittivity (εr) when filler loading approaches or exceeds the percolation threshold (fc), pristine Ni particles were covered by a layer of titanium dioxide (TiO2) shell via a sol–gel approach, and then they were composited with PVDF. The impacts of the TiO2 coating on the dielectric performances of the Ni/PVDF composites were explored as a function of the filler concentration, the shell thickness and frequency. In addition, the dielectric performances were fitted using the Havriliak–Negami (H–N) equation in order to further understand the TiO2 shell’s effect on polarization mechanism in the composites. The Ni@TiO2/PVDF composites exhibit high εr and enhanced breakdown strength (Eb) but remarkably suppressed loss and conductivity when compared with pristine Ni/PVDF because the TiO2 shell can efficiently stop the direct contact between Ni particles thereby suppressing the long–range electron transportation. Further, the dielectric performances can be effectively tuned through finely adjusting the TiO2 shell’ thickness. The resulting Ni@TiO2/PVDF composites with high εr and Eb but low loss show appealing applications in microelectronics and electrical fields

Influence of strain rate on grain refinement and texture evolution under complex shear stress conditions

The effects of different complex shear stress conditions on grain refinement and texture evolution of Mg-13Gd-4Y-2Zn-0.5Zr alloy were investigated. With increasing strain rate, the average grain size of compression-shear (CS) and compression-torsion (CT) samples are decreased, and the grain size of dynamic recrystallization (DRX) grains is also decreased. This is because that the precipitation number of β phases is increased, and the hindering effect on grain growth can be significantly enhanced. The DRX fractions of CS and CT samples are decreased with increased strain rate. The low DRX fraction at high strain rate is related to the insufficient time for grains to nucleate. The DRX process can be promoted by the PSN mechanism of second phases, and the grain growth can be restricted by the pinning effect. At the same time, the texture strength is enhanced as the strain rate increased. Besides, the kinking degree of lamellar long-period stacking ordered (LPSO) phases is increased. Under complex shear stress conditions, non-basal slip, especially pyramidal slip, is easily activated and the texture is deflected greatly. Compared with the CS samples, CT samples have smaller average grain size, higher DRX fraction, and lower texture strength for a certain strain rate. This is because that the equivalent stress of the CT sample is larger, the stress triaxiality is smaller, so more serious dislocations are piled up near grain boundaries and second phases. At the same time, since CT sample was sheared with torsion, the dislocation movement path can be called “rotational dislocation accumulation”, and the longer distribution path of the CT sample is generated, so more sub-grains and low-angle grain boundaries (LAGBs) are formed. Compared with the CS sample, more huge-angle grain boundaries (HAGBs) and DRX grains are formed from grain boundary to grain interior, so better grain refinement effect is achieved