Cell Structures in Heterologous Anti-Rabies Immunoglobulin Production

This paper reviews basic modern trends in obtainment of anti-rabies immunoglobulin with enhanced properties. One of these trends is associated with the substitution of organ-tissue antigen by cultural antigen at the stage of producer immunization. Analyzed are the results of research connected with the use of the cell-cultures grown Rabies virus in production of heterologous anti-rabies immunoglobulin. The study testifies of applicability of the transferred Vero cell culture as a substrate for rabies antigen preparation. In addition to this, specified are the advantages of the rabies cultural antigen usage over the organ-tissue one at the stage of hyper-immune serum production

Experimental Substantiation of Cultural Technologies Introduction into Manufacturing of Anti-Rabies Immunoglobulin

The review provides information on the major outcomes of research and development work, performed within the frames of the Federal Target Program “National system of chemical and biological safety” (2009-2014), aimed at elaboration and introduction of cultural techniques into the manufacturing of anti-rabies immunoglobulin. Described are the key phases in methodology engineering, deployed for the large-scale cultivation of fixed rabies virus, concentration of cultural liquid, quantitation of rabies virus using PCR, and immunization of producers. Obtained pilot batches of the enhanced anti-rabies immunoglobulin, complying with normative requirements to commercial formulations, testify to the effectiveness of the developed biotechnological and methodological procedures, as well as of the designed engineering-manufacturing project documentation

Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum

Nucleotide Sequence and Phylogenetic Analysis of Glycoprotein-G of the Russian Fixed Rabies Virus Strain “Moscow 3253”

Fully sequenced have been glycoprotein-G, sha-psi region, as well as H-end site of the L-gene in the rabies virus strain “Moscow 3253”. Compared are amino acid sequences of proteins of “Moscow 3253” strain and other fixed strains of the virus. Established is 98 % DNA homology with RV-97, and 91% homology with PV (Pasteur virus) strain. Constructed has been phylogenetic tree of the strain under study alongside with various groups of fixed rabies virus. It is revealed that “Moscow 3253” strain has closer genetic relations with Japanese group of strains, than with PV strain. Put forward is an assumption that PV strain does not derive from the virus isolated by Pasteur, but relates to the American group of strains

Cultural Antigen in the Technology for Anti-Rabies Immunoglobulin Obtainment from Equine Blood Serum

week after immunization (specific activity is identified using neutralization reaction on the model of white mice and dot-blot immunoassay). This level of activity is sufficient for the fractioning of immune serum and extraction of anti-rabies immunoglobulin. Physicochemical and biological properties of the anti-rabies immunoglobulin, obtained with the help of cultural antigen technique, meet the requirements stated in the normative documentation on anti-rabies immunoglobulins extracted from equine blood serum. Specific activity level of experimental batches of anti-rabies immunoglobulin, obtained with the help of cultural technologies, corresponds to 242 and 214 IU/ml

Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen

Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin

Promotion Effect of Alkali Metal Hydroxides on Polymer-Stabilized Pd Nanoparticles for Selective Hydrogenation of C–C Triple Bonds in Alkynols

Postimpregnation of Pd nanoparticles (NPs) stabilized within hyper-cross-linked polystyrene with sodium or potassium hydroxides of optimal concentration was found to significantly increase the catalytic activity for the partial hydrogenation of the C–C triple bond in 2-methyl-3-butyn-2-ol at ambient hydrogen pressure. The alkali metal hydroxide accelerates the transformation of the residual Pd(II) salt into Pd(0) NPs and diminishes the reaction induction period. In addition, the selectivity to the desired 2-methyl-3-buten-2-ol increases with the K- and Na-doped catalysts from 97.0 up to 99.5%. This effect was assigned to interactions of the alkali metal ions with the Pd NPs surfaces resulting in the sites’ separation and a change of reactants adsorption

Влияние степени окисления и распределения частиц рутения на гидрирование левулиновой кислоты в присутствии Ru-содержащих катализаторов на основе цеолитов

The paper presents data on the synthesis of ruthenium catalysts based on the zeolites HBeta, HΥ, HMordenite and HZSM‑5 and their testing in the hydrogenation reaction of levulinic acid to gamma- valerolactone in an aqueous medium at a temperature of 100 oC and partial hydrogen pressure of 1 MPa. For the initial zeolites and synthesized catalysts, physicochemical study was carried out using low- temperature nitrogen physisorption, XPS, DRIFT spectroscopy using CD3CN as a probe molecule, and electron microscopy. The dependence of the activity of Ru/zeolite composites on the surface content of RuO2 was establishedВ работе представлены данные по синтезу рутениевых катализаторов на основе цеолитов HBeta, HΥ, HMordenite и HZSM‑5 и их тестированию в реакции гидрирования левулиновой кислоты до гамма-валеролактона в водной среде при температуре 100 о С и парциальном давлении водорода 1 МПа. Для исходных цеолитов и катализаторов на их основе проведено физико-химическое исследование методами низкотемпературной адсорбции азота, РФЭС, ИК‑спектроскопии с адсорбцией CD3CN, а также электронной микроскопии. Установлена зависимость активности композитов Ru/цеолит от поверхностного содержания RuO

LASER-INDUCED THERMOTHERAPY AND PHOTODYNAMIC THERAPY IN DERMATOLOGY: OPPORTUNITIES AND FUTURE HORIZONS

The article reviews current data on the mechanisms of effects of laser radiation on the body, technical aspects of using different intensities of laser radiation and the effects of the laser on biological tissue. The results of studies of laser radiation as a part of comprehensive treatment of skin diseases including skin tumors are presented. Data on the efficacy and safety of laser therapy are discussed. Benefits of laser therapy in dermatology include shortening of treatment duration and only limited contraindications. Considering effectiveness, usability and safety of the method, it is regarded as a promising direction in dermatology