378 research outputs found
The exponential map for the unitary group SU(2,2)
In this article we extend our previous results for the orthogonal group,
, to its homomorphic group . Here we present a closed, finite
formula for the exponential of a traceless matrix, which can be
viewed as the generator (Lie algebra elements) of the group. We apply
this result to the group, which Lie algebra can be represented by the
Dirac matrices, and discuss how the exponential map for can be
written by means of the Dirac matrices.Comment: 10 page
The Exponential Map for the Conformal Group 0(2,4)
We present a general method to obtain a closed, finite formula for the
exponential map from the Lie algebra to the Lie group, for the defining
representation of the orthogonal groups. Our method is based on the
Hamilton-Cayley theorem and some special properties of the generators of the
orthogonal group, and is also independent of the metric. We present an explicit
formula for the exponential of generators of the groups, with , in particular we are dealing with the conformal group , which
is homomorphic to the group. This result is needed in the
generalization of U(1) gauge transformations to spin gauge transformations,
where the exponential plays an essential role. We also present some new
expressions for the coefficients of the secular equation of a matrix.Comment: 16pages,plain-TeX,(corrected TeX
DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and 1.0, 1.5 and 2.5 MV/m amplitudes, which do not affect cell growth and viability. A dose-dependent reduction in alkaline comet-assayed DNA migration is observed immediately after nsPEF exposure, accompanied by permeabilization of the plasma membrane (YO-PRO-1 uptake). Comet assay profiles return to normal within 60 minutes after pulse delivery at the highest pulse amplitude tested, indicating that our exposure protocol affects the nucleus, modifying DNA electrophoretic migration patterns
Value of systolic pulmonary arterial pressure as a prognostic factor of death in the systemic sclerosis EUSTAR population.
The aim of this study was to assess the prognostic value of systolic pulmonary artery pressure (sPAP) estimated by echocardiography in the multinational European League Against Rheumatism Scleroderma Trial and Research (EUSTAR) cohort.Data for patients with echocardiography documented between 1 January 2005 and 31 December 2011 were extracted from the EUSTAR database. Stepwise forward multivariable statistical Cox pulmonary hypertension analysis was used to examine the independent effect on survival of selected variables.Based on our selection criteria, 1476 patients were included in the analysis; 87\% of patients were female, with a mean age of 56.3 years (s.d. 13.5) and 31\% had diffuse SSc. The mean duration of follow-up was 2.0 years (s.d. 1.2, median 1.9). Taking index sPAP of 50 mmHg. In a multivariable Cox model, sPAP and the diffusing capacity for carbon monoxide (DLCO) were independently associated with the risk of death [HR 1.833 (95\% CI 1.035, 3.247) and HR 0.973 (95\% CI 0.955, 0.991), respectively]. sPAP was an independent risk factor for death with a HR of 3.02 (95\% CI 1.91, 4.78) for sPAP â„36 mmHg.An estimated sPAP >36 mmHg at baseline echocardiography was significantly and independently associated with reduced survival, regardless of the presence of pulmonary hypertension based on right heart catheterization
Occupational exposure to electromagnetic fields in magnetic resonance environment: an update on regulation, exposure assessment techniques, health risk evaluation, and surveillance
Magnetic resonance imaging (MRI) is one of the most-used diagnostic imaging methods worldwide. There are âŒ50,000 MRI scanners worldwide each of which involves a minimum of five workers from different disciplines who spend their working days around MRI scanners. This review analyzes the state of the art of literature about the several aspects of the occupational exposure to electromagnetic fields (EMF) in MRI: regulations, literature studies on biological effects, and health surveillance are addressed here in detail, along with a summary of the main approaches for exposure assessment. The original research papers published from 2013 to 2021 in international peer-reviewed journals, in the English language, are analyzed, together with documents published by legislative bodies. The key points for each topic are identified and described together with useful tips for precise safeguarding of MRI operators, in terms of exposure assessment, studies on biological effects, and health surveillance. Graphical abstract: [Figure not available: see fulltext.
Building nonparametric -body force fields using Gaussian process regression
Constructing a classical potential suited to simulate a given atomic system
is a remarkably difficult task. This chapter presents a framework under which
this problem can be tackled, based on the Bayesian construction of
nonparametric force fields of a given order using Gaussian process (GP) priors.
The formalism of GP regression is first reviewed, particularly in relation to
its application in learning local atomic energies and forces. For accurate
regression it is fundamental to incorporate prior knowledge into the GP kernel
function. To this end, this chapter details how properties of smoothness,
invariance and interaction order of a force field can be encoded into
corresponding kernel properties. A range of kernels is then proposed,
possessing all the required properties and an adjustable parameter
governing the interaction order modelled. The order best suited to describe
a given system can be found automatically within the Bayesian framework by
maximisation of the marginal likelihood. The procedure is first tested on a toy
model of known interaction and later applied to two real materials described at
the DFT level of accuracy. The models automatically selected for the two
materials were found to be in agreement with physical intuition. More in
general, it was found that lower order (simpler) models should be chosen when
the data are not sufficient to resolve more complex interactions. Low GPs
can be further sped up by orders of magnitude by constructing the corresponding
tabulated force field, here named "MFF".Comment: 31 pages, 11 figures, book chapte
miRNA in situ hybridization in circulating tumor cells - MishCTC
irculating tumor cells (CTCs) must be phenotypically and genetically characterized before they can be utilized in clinical applications. Here, we present the first protocol for the detection of miRNAs in CTCs using in situ hybridization (ISH) combined with immunomagnetic selection based on cytokeratin (CK) expression and immunocytochemistry. Locked-Nucleic Acid (LNA) probes associated with an enzyme-labeled fluorescence (ELF) signal amplification approach were used to detect miRNA-21 in CTCs. This protocol was optimized using both epithelial tumor (MDA-MB468) and epithelial non-tumor (MCF-10A) cell lines, and miRNA-21 was selected as the target miRNA because of its known role as an onco-miRNA. Hematopoietic cells do not express miRNA-21; thus, miRNA-21 is an ideal marker for detecting CTCs. Peripheral blood samples were taken from 25 cancer patients and these samples were analyzed using our developed protocol. Of the 25 samples, 11 contained CTCs. For all 11 CTC-positive samples, the isolated CTCs expressed both CK and miRNA-21. Finally, the protocol was applied to monitor miRNA-21 expression in epithelial to mesenchymal transition (EMT)-induced MCF-7 cells, an epithelial tumor cell line. CK expression was lost in these cells, whereas miRNA-21 was still expressed, suggesting that miRNA-21 might be a good marker for detecting CTCs with an EMT phenotype.JJDM thanks the Spanish Ministerio de EconomĂa y Competitividad for a RamĂłn y Cajal
Fellowship (Grant CTQ2012-34778). This research was partially supported by Marie Curie Career Integration Grants within the 7th European Community Framework Program
(FP7-PEOPLE-2011-CIG-Project Number 294142 to RMSM and FP7-PEOPLE-2012-CIG-Project Number 322276 toJJDM). Thisresearch wasalso partially
supported by Consejeria de Salud de la Junta de Andalucı
Ì
a (PI0294-2012)
Ipsilateral lower extremity joint involvement increases the risk of poor pain and function outcomes after hip or knee arthroplasty
A reconstruction technique for long-range stimulated Brillouin scattering distributed fibre-optic sensors: experimental results
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