9 research outputs found
Automatic detection of low surface brightness galaxies from SDSS images
Low surface brightness (LSB) galaxies are galaxies with central surface
brightness fainter than the night sky. Due to the faint nature of LSB galaxies
and the comparable sky background, it is difficult to search LSB galaxies
automatically and efficiently from large sky survey. In this study, we
established the Low Surface Brightness Galaxies Auto Detect model (LSBG-AD),
which is a data-driven model for end-to-end detection of LSB galaxies from
Sloan Digital Sky Survey (SDSS) images. Object detection techniques based on
deep learning are applied to the SDSS field images to identify LSB galaxies and
estimate their coordinates at the same time. Applying LSBG-AD to 1120 SDSS
images, we detected 1197 LSB galaxy candidates, of which 1081 samples are
already known and 116 samples are newly found candidates. The B-band central
surface brightness of the candidates searched by the model ranges from 22 mag
arcsec to 24 mag arcsec , quite consistent with the
surface brightness distribution of the standard sample. 96.46\% of LSB galaxy
candidates have an axis ratio () greater than 0.3, and 92.04\% of them
have \textless 0.4, which is also consistent with the standard
sample. The results show that the LSBG-AD model learns the features of LSB
galaxies of the training samples well, and can be used to search LSB galaxies
without using photometric parameters. Next, this method will be used to develop
efficient algorithms to detect LSB galaxies from massive images of the next
generation observatories.Comment: 11 pages, 9 figures,accepted to be published on MNRA
DEM investigation on the breakage of spherical aggregate under multi-contact loadings
Particle crushing is commonly encountered during the storage, transportation, and handling of granular assemblies. This work is aimed at ascertaining the applicability of four particle breakage criteria frequently mentioned in literature. Discrete element method (DEM) simulations were conducted to investigate the crushing of spherical aggregates under multicontact loadings. Mean and major principal stress criteria, octahedral shear stress criterion, and maximal contact force (MCF) criterion were then evaluated based on the obtained DEM results. It is found that the first three parameters all vary with the number of loading contacts, demonstrating they cannot predict the crushing of particles under arbitrary loading configuration. Simulation results indicate that the MCF at crushing is related to the number and the spatial arrangement of loading contacts. Thus, strictly speaking, this parameter cannot uniquely define particle breakage either. The influences of the microstructural heterogeneity on the breakage strength of particle and also on the applicability of MCF criterion are discussed
Light and Potassium Improve the Quality of <i>Dendrobium officinale</i> through Optimizing Transcriptomic and Metabolomic Alteration
Background: Dendrobium officinale is a perennial epiphytic herb in Orchidaceae. Cultivated products are the main alternative for clinical application due to the shortage of wild resources. However, the phenotype and quality of D. officinale have changed post-artificial cultivation, and environmental cues such as light, temperature, water, and nutrition supply are the major influencing factors. This study aims to unveil the mechanisms beneath the cultivation-induced variation by analyzing the changes of the metabolome and transcriptome of D. officinale seedlings treated with red- blue LED light and potassium fertilizer. Results: After light- and K-treatment, the D. officinale pseudobulbs turned purple and the anthocyanin content increased significantly. Through wide-target metabolome analysis, compared with pseudobulbs in the control group (P), the proportion of flavonoids in differentially-accumulated metabolites (DAMs) was 22.4% and 33.5% post light- and K-treatment, respectively. The gene modules coupled to flavonoids were obtained through the coexpression analysis of the light- and K-treated D. officinale transcriptome by WGCNA. The KEGG enrichment results of the key modules showed that the DEGs of the D. officinale pseudobulb were enriched in phenylpropane biosynthesis, flavonoid biosynthesis, and jasmonic acid (JA) synthesis post-light- and K-treatment. In addition, anthocyanin accumulation was the main contribution to the purple color of pseudobulbs, and the plant hormone JA induced the accumulation of anthocyanins in D. officinale. Conclusions: These results suggested that light and potassium affected the accumulation of active compounds in D. officinale, and the gene-flavone network analysis emphasizes the key functional genes and regulatory factors for quality improvement in the cultivation of this medicinal plant
Photothermal Regulated Nanozyme of CuFeS<sub>2</sub> Nanoparticles for Efficiently Promoting Wound Healing Infected by Multidrug Resistant Bacteria
Peroxidase-mediated chemokinetic therapy (CDT) can effectively resist bacteria; however, factors such as the high dosage of drugs seriously limit the antibacterial effect. Herein, CuFeS2 nanoparticles (NPs) nanozyme antibacterial system with the photothermal effect and peroxidase-like catalytic activity are proposed as a combined antibacterial agent with biosafety, high-efficiency, and broad-spectrum antibacterial ability. In addition, the as-obtained CuFeS2 NPs with a low doses of Cu+ and Fe3+ can change the permeability of bacterial cell membranes and break the antioxidant balance by consuming intracellular glutathione (GSH), which results in more conducive ROS production. Meanwhile, the photothermal heating can regulate the CuFeS2 NPs close to their optimal reaction temperature (60 °C) to release more hydroxyl radical in low concentrations of H2O2 (100 ÂľM). The proposed CuFeS2 NPs-based antibacterial system achieve more than 99% inactivation efficiency of methicillin-resistant Staphylococcus aureus (106 CFU mLâ1 MRSA), hyperspectral bacteria β-Escherichia coli (106 CFU mLâ1 ESBL) and Pseudomonas aeruginosa (106 CFU mLâ1 PA), even at low concentration (2 Îźg mLâ1), which is superior to those of the conventional CuO NPs at 4 mg mLâ1 reported in the literature. In vivo experiments further confirm that CuFeS2 NPs can effectively treat wounds infected by MRSA and promote the wound healing. This study demonstrates that excellent antibacterial ability and good biocompatibility make CuFeS2 NPs a potential anti-infection nanozyme with broad application prospects
Olfactory Ensheathing Cells Alleviate Facial Pain in Rats with Trigeminal Neuralgia by Inhibiting the Expression of P2X7 Receptor
Trigeminal neuralgia (TN) is a common facial neuropathic pain that is mainly characterized by spontaneous or induced needling or electric shock pain in the innervation area of the trigeminal nerve. It is also referred to as “the cancer that never dies”. The olfactory ensheathing cell (OEC) is a special glial cell in the nervous system that has a strong supportive function in nerve regeneration. Cell transplantation therapy is a useful treatment modality that we believe can be applied in TN management. In this study, OECs were transplanted into the ligation site of the infraorbital nerve of rats. We found that after the OEC transplantation, mechanical pain threshold in the face of the rats was significantly increased. Western blotting, immunofluorescence assay, and reverse transcription-quantitative polymerase chain reaction were performed on the trigeminal ganglia (TG) of model rats. The results revealed a decrease in the expression of P2X7 receptor (P2X7R) in the trigeminal ganglia. Our findings show that OEC transplantation has a good therapeutic effect on TN in rats, and that can reduce the expression of P2X7R in trigeminal ganglia. Therefore, we think that OEC transplantation may be a suitable treatment for TN
Exploring Information Exchange between <i>Thesium chinense</i> and Its Host <i>Prunella vulgaris</i> through Joint Transcriptomic and Metabolomic Analysis
Background: Thesium chinense known as the âplant antibioticâ is a facultative root hemi-parasitic herb while Prunella vulgaris can serve as its host. However, the molecular mechanisms underlying the communication between T. chinense and its host remained largely unexplored. The aim of this study was to provide a comprehensive view of transferred metabolites and mobile mRNAs exchanged between T. chinense and P. vulgaris. Results: The wide-target metabolomic and transcriptomic analysis identified 5 transferred metabolites (ethylsalicylate, eriodictyol-7-O-glucoside, aromadendrin-7-O-glucoside, pruvuloside B, 2-ethylpyrazine) and 50 mobile genes between T. chinense and P. vulgaris, as well as haustoria formation related 56 metabolites and 44 genes. There were 4 metabolites (ethylsalicylate, eriodictyol-7-O-glucoside, aromadendrin-7-O-glucoside and pruvuloside B) that are transferred from P. vulgaris to T. chinense, whereas 2-ethylpyrazine was transferred in the opposite direction. Furthermore, we inferred a regulatory network potentially involved in haustoria formation, where three metabolites (N,Nâ˛-Dimethylarginine/SDMA, NG,NG-Dimethyl-L-arginine, 2-Acetoxymethyl-anthraquinone) showed significant positive correlations with the majority of haustoria formation-related genes. Conclusions: These results suggested that there was an extensive exchange of information with P. vulgaris including transferred metabolites and mobile mRNAs, which might facilitate the haustoria formation and parasition of T. chinense
Effects of Drip Tape Layout and Flow Rate on Water and Nitrogen Distributions within the Root Zone and Summer Maize Yield in Sandy Tidal Soil
Drip tape layout and flow rate are crucial variables that impact the effects of drip fertigation. To investigate the influence of drip tape layout and flow rate on the soil water and nitrogen transport in summer maize in sandy tidal soil, field experiments were conducted for two years. Two drip tape layouts were set: one tape serving for two crop rows (N) and one tape serving for each crop row (E), with two levels of drip flow rate, i.e., high (2 L/h; H) and low (1.3 L/h; L). The results show that under the same drip tape layout, the lower the drip emitter flow rate, the more upright the shape of wetted soil volume. The maximum vertical and horizontal water transport distance under NL treatment was higher than that under NH, EH, and EL treatments. After surface drip fertigation, nitrate nitrogen accumulated near and at the edge of the wetted soil volume. In 2020, under NL treatment, nitrate nitrogen transported to a 55 cm soil layer, which was 22.22%, 71.42%, and 57.14% deeper than that under NH, EH, and EL treatments, respectively. In 2021, nitrate nitrogen could transport to a 60 cm soil layer in both NL and NH treatments. The maximum concentration of ammonium nitrogen was nearby the emitter. Under NL treatment, ammonium nitrogen was transported to 48 and 60 cm soil layers below the emitter in 2020 and 2021, respectively, which was deeper than that observed under NH, EH, and EL treatments. The soil inorganic nitrogen residue of the NL was lower than that of the NH, EH, and EL treatments. Compared with NH, EH, and EL treatments, the two-year maize yield under NL treatment increased by 11.09%, 13.47%, and 8.66% on average, respectively. NL treatment exhibited the highest water use efficiency and nitrogen fertilizer productivity. Therefore, NL treatment (one drip tape serving for two rows with 1.3 L/h flow rate) could promote the absorption of water and nutrients, reduce inorganic nitrogen residue, and to obtain high maize yield in sandy tidal soil
Table_1_Drought stress modifies the community structure of root-associated microbes that improve Atractylodes lancea growth and medicinal compound accumulation.xlsx
Atractylodes lancea is an important medicinal plant in traditional Chinese medicine, its rhizome is rich of volatile secondary metabolites with medicinal values and is largely demanded in modern markets. Currently, supply of high-yield, high-quality A. lancea is mainly achieved via cultivation. Certain soil microbes can benefit plant growth, secondary metabolism and induce resistance to environmental stresses. Hence, studies on the effects of soil microbe communities and isolates microorganisms on A. lancea is extremely meaningful for future application of microbes on cultivation. Here we investigated the effects of the inoculation with an entire soil microbial community on the growth, resistance to drought, and accumulation of major medicinal compounds (hinesol, β-eudesmol, atractylon and atractylodin) of A. lancea. We analyzed the interaction between A. lancea and the soil microbes at the phylum and genus levels under drought stress of different severities (inflicted by 0%, 10% and 25% PEG6000 treatments). Our results showed that inoculation with soil microbes promoted the growth, root biomass yield, medicinal compound accumulation, and rendered drought-resistant traits of A. lancea, including relatively high root:shoot ratio and high root water content under drought. Moreover, our results suggested drought stress was more powerful than the selectivity of A. lancea in shaping the root-associated microbial communities; also, the fungal communities had a stronger role than the bacterial communities in protecting A. lancea from drought. Specific microbial clades that might have a role in protecting A. lancea from drought stress were identified: at the genus level, the rhizospheric bacteria Bacillus, Dylla and Actinomadura, and rhizospheric fungi Chaetomium, Acrophialophora, Trichoderma and Thielava, the root endophytic bacteria Burkholderia-Caballeronia-Paraburkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Dylla and Actinomadura, and the root endophytic fungus Fusarium were closely associated with A. lancea under drought stress. Additionally, we acquired several endophytic Paenibacillus, Paraburkholderia and Fusarium strains and verified they had differential promoting effects on the medicinal compound accumulation in A. lancea root. This study reports the interaction between A. lancea and soil microbe communities under drought stress, and provides insights for improving the outcomes in A. lancea farming via applying microbe inoculation.</p
DataSheet_1_Drought stress modifies the community structure of root-associated microbes that improve Atractylodes lancea growth and medicinal compound accumulation.docx
Atractylodes lancea is an important medicinal plant in traditional Chinese medicine, its rhizome is rich of volatile secondary metabolites with medicinal values and is largely demanded in modern markets. Currently, supply of high-yield, high-quality A. lancea is mainly achieved via cultivation. Certain soil microbes can benefit plant growth, secondary metabolism and induce resistance to environmental stresses. Hence, studies on the effects of soil microbe communities and isolates microorganisms on A. lancea is extremely meaningful for future application of microbes on cultivation. Here we investigated the effects of the inoculation with an entire soil microbial community on the growth, resistance to drought, and accumulation of major medicinal compounds (hinesol, β-eudesmol, atractylon and atractylodin) of A. lancea. We analyzed the interaction between A. lancea and the soil microbes at the phylum and genus levels under drought stress of different severities (inflicted by 0%, 10% and 25% PEG6000 treatments). Our results showed that inoculation with soil microbes promoted the growth, root biomass yield, medicinal compound accumulation, and rendered drought-resistant traits of A. lancea, including relatively high root:shoot ratio and high root water content under drought. Moreover, our results suggested drought stress was more powerful than the selectivity of A. lancea in shaping the root-associated microbial communities; also, the fungal communities had a stronger role than the bacterial communities in protecting A. lancea from drought. Specific microbial clades that might have a role in protecting A. lancea from drought stress were identified: at the genus level, the rhizospheric bacteria Bacillus, Dylla and Actinomadura, and rhizospheric fungi Chaetomium, Acrophialophora, Trichoderma and Thielava, the root endophytic bacteria Burkholderia-Caballeronia-Paraburkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Dylla and Actinomadura, and the root endophytic fungus Fusarium were closely associated with A. lancea under drought stress. Additionally, we acquired several endophytic Paenibacillus, Paraburkholderia and Fusarium strains and verified they had differential promoting effects on the medicinal compound accumulation in A. lancea root. This study reports the interaction between A. lancea and soil microbe communities under drought stress, and provides insights for improving the outcomes in A. lancea farming via applying microbe inoculation.</p