A Narrative Review on Oral and Periodontal Bacteria Microbiota Photobiomodulation, through Visible and Near-Infrared Light: From the Origins to Modern Therapies

Photobiomodulation (PBM) consists of a photon energy transfer to the cell, employing non-ionizing light sources belonging to the visible and infrared spectrum. PBM acts on some intrinsic properties of molecules, energizing them through specific light wavelengths. During the evolution of life, semiconducting minerals were energized by sun radiation. The molecules that followed became photoacceptors and were expressed into the first proto-cells and prokaryote membranes. Afterward, the components of the mitochondria electron transport chain influenced the eukaryotic cell physiology. Therefore, although many organisms have not utilized light as an energy source, many of the molecules involved in their physiology have retained their primordial photoacceptive properties. Thus, in this review, we discuss how PBM can affect the oral microbiota through photo-energization and the non-thermal effect of light on photoacceptors (i.e., cytochromes, flavins, and iron-proteins). Sometimes, the interaction of photons with pigments of an endogenous nature is followed by thermal or photodynamic-like effects. However, the preliminary data do not allow determining reliable therapies but stress the need for further knowledge on light-bacteria interactions and microbiota management in the health and illness of patients through PBM

Basis of economic security of a highly qualified athlete

At the present stage issues of ensuring personal economic security are becoming particularly relevant and especially it is important in the context of local and global pandemics. This problem area has a complex character, and it is characterized by its multiplicity that determines the necessity to study the relevant issues in the context of the professional identity of the individual. From our point of view the most interesting feature is the development of problems of personal economic security in the physical culture and sports industry. It is worth taking into account the fact that the problems of ensuring economic security in the physical culture and sports industry are in most cases considered at the level of major manufacturers of sports products, international sports organizations and the state. In this regard the issues of ensuring personal economic security of professional athletes as subjects of international sports remain largely outside the scope of these studies. This circumstance clearly demonstrates the fact that there is a “dialectical gap” between the sphere of economic security in the physical culture and sports industry and the issues of personal economic security of professional athletes that directly influences on the prospects for the development of spectator sports and sports of higher achievements

Optimizing sgRNA to Improve CRISPR/Cas9 Knockout Efficiency: Special Focus on Human and Animal Cell

During recent years, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technologies have been noticed as a rapidly evolving tool to deliver a possibility for modifying target sequence expression and function. The CRISPR/Cas9 tool is currently being used to treat a myriad of human disorders, ranging from genetic diseases and infections to cancers. Preliminary reports have shown that CRISPR technology could result in valued consequences for the treatment of Duchenne muscular dystrophy (DMD), cystic fibrosis (CF), β-thalassemia, Huntington’s diseases (HD), etc. Nonetheless, high rates of off-target effects may hinder its application in clinics. Thereby, recent studies have focused on the finding of the novel strategies to ameliorate these off-target effects and thereby lead to a high rate of fidelity and accuracy in human, animals, prokaryotes, and also plants. Meanwhile, there is clear evidence indicating that the design of the specific sgRNA with high efficiency is of paramount importance. Correspondingly, elucidation of the principal parameters that contributed to determining the sgRNA efficiencies is a prerequisite. Herein, we will deliver an overview regarding the therapeutic application of CRISPR technology to treat human disorders. More importantly, we will discuss the potent influential parameters (e.g., sgRNA structure and feature) implicated in affecting the sgRNA efficacy in CRISPR/Cas9 technology, with special concentration on human and animal studies

Current Trends in Bone Augmentation Techniques and Dental Implantology: An Editorial Overview

Dental implants and related bone augmentation problems have seen major progress since early protocols were tested in the 1980s [...

Learning Management Systems in Academic and Corporate Distance Education

Present-day development of information technologies is making deeper and deeper incursions into all spheres of human activity, both social and economic. It is only natural that they greatly influence the educational industry, changing the approaches, methods and forms of education. Currently e-learning and distance technologies are being actively introduced into both academic and corporate education, as complementary to traditional forms. The efforts of teachers are aimed at finding effective models of blended learning. All this determines the relevance of the present study. The abstracted article is aimed at developing frameworks of a distance learning model based on the use of modern learning management systems. To obtain thorough and reliable results the authors make use of two research approaches: acmeological and competence-based. They review the features, the scope and use of the existing LMS platforms and work out tools for the development of e-learning system. In addition, they assess the compliance of e-learning tools with the preferences of X, Y and Z generation users. The outcome of the research is the development of a universal competency that can be applied to the study of modern methods and approaches with reference to both academic teaching and learning, and to corporate training. It is quite apparent that pros and cons of the learning management system and its possibilities should be carefully studied and thoughtfully used. The effective e-learning requires constant monitoring of equipment, proper work of host servers and considering user-friendliness of the platforms. This is related to the fact that public educational organizations, including schools, colleges and universities, especially in low developed countries, prefer traditional teaching methods and are slowly moving to innovative models. The research findings can be viewed as a step forward in creating user-friendly and flexible e-learning platforms, suitable for separate users, institutions and businesses. They can be applied for further assessment of existing learning models, especially for the younger generation, and for research and development of new strategies and methods of using learning management systems and platforms

N-lump and interaction solutions of localized waves to the (2

With the aid of the binary Hirota polynomial scheme, the bilinear form of the generalized (3 + 1)-dimensional Kadomtsev-Petviashvili equation, is constructed. Then, several classes of rogue waves-type solutions to the generalized (3 + 1)-dimensional Kadomtsev-Petviashvili equation within the frame of the bilinear equation are found. Finally, M-soliton solution and N-soliton based on the frame of the bilinear and expansion of summation multiple soliton solutions were used to get different types of k-solitons waves of this considered model. The physical phenomena of these gained multiple soliton solutions are analyzed and indicated in figures by selecting suitable values. These results can help us better understand interesting physical phenomena and mechanism

Photobiomodulation and Oxidative Stress: 980 nm Diode Laser Light Regulates Mitochondrial Activity and Reactive Oxygen Species Production

Photobiomodulation with 808 nm laser light electively stimulates Complexes III and IV of the mitochondrial respiratory chain, while Complexes I and II are not affected. At the wavelength of 1064 nm, Complexes I, III, and IV are excited, while Complex II and some mitochondrial matrix enzymes seem to be not receptive to photons at that wavelength. Complex IV was also activated by 633 nm. The mechanism of action of wavelengths in the range 900–1000 nm on mitochondria is less understood or not described. Oxidative stress from reactive oxygen species (ROS) generated by mitochondrial activity is an inescapable consequence of aerobic metabolism. The antioxidant enzyme system for ROS scavenging can keep them under control. However, alterations in mitochondrial activity can cause an increment of ROS production. ROS and ATP can play a role in cell death, cell proliferation, and cell cycle arrest. In our work, bovine liver isolated mitochondria were irradiated for 60 sec, in continuous wave mode with 980 nm and powers from 0.1 to 1.4 W (0.1 W increment at every step) to generate energies from 6 to 84 J, fluences from 7.7 to 107.7 J/cm2, power densities from 0.13 to 1.79 W/cm2, and spot size 0.78 cm2. The control was equal to 0 W. The activity of the mitochondria’s complexes, Krebs cycle enzymes, ATP production, oxygen consumption, generation of ROS, and oxidative stress were detected. Lower powers (0.1–0.2 W) showed an inhibitory effect; those that were intermediate (0.3–0.7 W) did not display an effect, and the higher powers (0.8–1.1 W) induced an increment of ATP synthesis. Increasing the power (1.2–1.4 W) recovered the ATP production to the control level. The interaction occurred on Complexes III and IV, as well as ATP production and oxygen consumption. Results showed that 0.1 W uncoupled the respiratory chain and induced higher oxidative stress and drastic inhibition of ATP production. Conversely, 0.8 W kept mitochondria coupled and induced an increase of ATP production by increments of Complex III and IV activities. An augmentation of oxidative stress was also observed, probably as a consequence of the increased oxygen consumption and mitochondrial isolation experimental conditions. No effect was observed using 0.5 W, and no effect was observed on the enzymes of the Krebs cycle

Photobiomodulation Therapy through a Novel Flat-Top Hand-Piece Prototype Improves Tissue Regeneration in Amphioxus (Branchiostoma lanceolatum): Proposal of an Ethical Model for Preclinical Screening

Despite the literature providing compelling evidence for the medical efficacy of photobiomodulation (PBM) therapy, its consistency in terms of accuracy and standardization needs improving. Identification of new technology and reliable and ethical biological models is, therefore, a challenge for researchers working on PBM. We tested the reliability of PBM irradiation through a novel delivery probe with a flat-top beam profile on the regenerating amphioxus Branchiostoma lanceolatum. The caudalmost 9 ± 1 myotomes, posterior to the anus, were excised using a sterile lancet. Animals were randomly split into three experimental groups. In the control group, the beam area was bounded with the 635-nm red-light pointer (negligible power, <0.5 mW) and the laser device was coded to irradiate 810 nm and 0 W. In Group laser-1, the beam area was bounded with the same 635-nm red-light pointer and irradiated at 810 nm, 1 W in CW for 60 s, spot-size 1 cm2, 1 W/cm2, 60 J/cm2, and 60 J; irradiation was performed every day for two weeks. In Group laser-2, the beam area was bounded with the same 635-nm red-light pointer and irradiated at 810 nm, 1 W in CW for 60 s, spot-size 1 cm2, 1 W/cm2, 60 J/cm2, and 60 J; irradiation was performed on alternate days for four weeks. We observed that PBM improved the natural wound-healing and regeneration process. The effect was particularly evident for the notochord. Daily irradiation better supported the regenerative process