Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis

The article presents the results of studying the role of erythrocyte and leukocyte indices of intoxication in the pathogenesis of experimental peritonitis. When studying the role of erythrocyte and leukocyte indices, a unidirectional trend of their change was revealed. An increase in the erythrocyte index of intoxication in the pathogenesis of experimental fecal peritonitis was established, most pronounced on the 10th day of the experiment (the level of EII increased by 107% compared to intact animals). The results of the study of the leukocyte intoxication index indicate a unidirectional trend, as in the case of the erythrocyte intoxication index. On the first day, the leukocyte index of intoxication increased on 225% (р<0.05), on the 4th day – on 256.7% (р<0.05), and on the 10th day – on 260% (р <0.05) compared to intact animals. It has been proven that the leukocyte index of intoxication is a more sensitive marker compared to the erythrocyte index

Research of effects by donators nitrogen of oxygen in complex therapy during experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups:Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis.Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction  correction with the use of a nitric oxide donor.Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per100 gof animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826).The following results were obtained.Histologically confirmed the development of experimental peritonitis in all groups of the experiment. In the group №2 on the third day the deterioration of the studied structures was established. In the group in which the experimental peritonitis was corrected by antibiotic therapy and chlorhexidine remediation, the effectiveness of the correction was morphologically proven. In group 4, macroscopic and microscopic examinations of the intestine, liver, and abdomen did not reveal significant differences from the histological picture we observed in rats of the intact group

Vasoconsrictive and vasodilatative imbalance at experimental fecal peritonitis

Peritonitis problem today one of the most urgent issues in the field of clinical pathological physiology and abdominal surgery. According to various data, mortality in peritonitis is 18.3 - 62.8%. Endothelial dysfunction is the main cause of cardiovascular diseases and death in patients after postponed peritonitis. The aim of the study – investigation of vasoconstrictive and vasodilatative potential in the pathogenesis of experimental peritonitis and its correction. It has been proved that experimental fecal peritonitis development pathologically affects the vessels functional state at each of the research stages. It's established weakening of the vasodilatative process on the background of peritonitis development, as evidenced by a decrease in the content of S-nitrosothiols. Result of this work has been proved that in peritonitis pathogenesis the vasoconstrictor potential of the vessels is significantly increased, as evidenced by a significant increase in endothelin-1 in animals that have been modeled by fecal peritonitis. Nitric oxide donor efficiency has been proved as part of complex correction of peritonitis and endothelial dysfunction as its complication. The most pronounced positive effect was detected at the 21st day, which indicates the expediency of long-term use of L-arginine as a remedy for correction

Vasoconsrictive and vasodilatative imbalance at experimental fecal peritonitis

Peritonitis problem today one of the most urgent issues in the field of clinical pathological physiology and abdominal surgery. According to various data, mortality in peritonitis is 18.3 - 62.8%. Endothelial dysfunction is the main cause of cardiovascular diseases and death in patients after postponed peritonitis. The aim of the study – investigation of vasoconstrictive and vasodilatative potential in the pathogenesis of experimental peritonitis and its correction. It has been proved that experimental fecal peritonitis development pathologically affects the vessels functional state at each of the research stages. It's established weakening of the vasodilatative process on the background of peritonitis development, as evidenced by a decrease in the content of S-nitrosothiols. Result of this work has been proved that in peritonitis pathogenesis the vasoconstrictor potential of the vessels is significantly increased, as evidenced by a significant increase in endothelin-1 in animals that have been modeled by fecal peritonitis. Nitric oxide donor efficiency has been proved as part of complex correction of peritonitis and endothelial dysfunction as its complication. The most pronounced positive effect was detected at the 21st day, which indicates the expediency of long-term use of Larginine as a remedy for correction

Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups: Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis. Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor. Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826). The following results were obtained. Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis. Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001). It was revealed significant increase in erythrocyte intoxication index (EII). It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis. It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed

Прогностическая модель развития эндотелиальной дисфункции при экспериментальном перитоните

Для прогнозування виникнення ендотеліальної дисфункції (ЕД) використовувався метод логістичної регресії. У якості факторів-предикторів були використані лейкоцитарний та еритроцитарний індекси інтоксикації і фактор Віллебранда. В результаті дослідження було встановлено, що розроблена модель в 91,30% випадків вірно передбачає виникнення ЕД. Відсоток хибних передбачень складає 8,70%. При цьому в 92,59% випадків модель вірно передбачає відсутність виникнення ЕД, частка хибних передбачень складає відповідно 7,41%. Можемо стверджувати, що така якість прогнозу дозволяє використання даної моделі у практиці. Ключові слова: перитоніт, експеримент, прогностична модель, ендогенна інтоксикація, ендотеліальна дисфункціяWe used logistic regression method to predict occurrence on endothelial dysfunction (ED).The following factors were used as predictors: leukocyte index on intoxication (LII), erythrocyte index on intoxication (EII) and von Willebrand Factor (VWF). It was found that the prognostic model developed by us in 91.30% on cases correctly suggests the emergence on ED. In 92.59% on cases the model correctly assumes the absence on ED, the proportion on false predictionsis is 7.41%. This prognosis quality allows us use this model in practice.Для прогнозирования возникновения эндотелиальной дисфункции использовался метод логистической регрессии. В качестве факторов-предикторов были использованы лейкоцитарный и эритроцитарный индексы интоксикации и фактор Виллебранда. В результате исследования было установлено, что разработанная модель в 91,30% случаев верно предполагает возникновение эндотелиальной дисфункции.Процент ложных предсказаний составляет 8,70%. При этом в 92,59% случаев модель верно предполагает отсутствие возникновения ЭД, доля ошибочных предсказаний составляет соответственно 7,41%. Можем утверждать, что такое качество прогноза позволяет использование данной модели на практике

Research of effects by donators nitrogen of oxygen in complex therapy during experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals – 180–220 g. Animals were divided into 4 groups: Group 1 – 20 intact animals. Group 2 – 50 rats with simulated fecal peritonitis. Group 3 – 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 – 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor.Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826). The following results were obtained. Histologically confirmed the development of experimental peritonitis in all groups of the experiment. In the group №2 on the third day the deterioration of the studied structures was established. In the group in which the experimental peritonitis was corrected by antibiotic therapy and chlorhexidine remediation, the effectiveness of the correction was morphologically proven. In group 4, macroscopic and microscopic examinations of the intestine, liver, and abdomen did not reveal significant differences from the histological picture we observed in rats of the intact group

Исследование динамики эндотелина-1 в патогенезе экспериментального перитонита и под влиянием различных способов коррекции

Background. Currently, one of the most serious complications of acute inflammatory diseases of the abdominal cavity is peritonitis. Objective: to study the development of endothelial dysfunction in experimental peritonitis and compare its correction with a solution of decamethoxine, aminoguadine and L-arginine. Materials and methods of research. The study was conducted on 260 white rats of reproductive age (3 months), animal weight — 180–220 g. Animals were divided into 4 groups. Fecal peritonitis was simulated by introducing a 10 % fecal suspension at a dose of 0.5 ml per 100 g of animal weight to the abdominal cavity of laboratory animals by puncture method. Research results. Pathological increase in vasoconstriction caused by experimental peritonitis was revealed. It was found that the use of solution, consisting of decamethoxine (10 mg/50 ml of solution) and sodium hyaluronate, is an effective method of rehabilitation and the basis for further correction of complications of experimental fecal peritonitis.The use of aminoguadine in combination with the rehabilitation of the abdominal cavity with a solution of decamethoxine had a positive effect on reducing the level of endothelin-1 in the simulated peritonitis. Combination of nitric oxide donor with decamethoxine solution and sodium hyaluronate nitric oxide donor effect on reducing the level of endothelin-1 in the simulated peritonitis. Conclusions: Given the influence of endogenous intoxication on the increase of the marker of vasoconstriction, rehabilitation of the abdominal cavity, the choice of decamethoxine solution (10 mg/50 ml of solution) in the conditions of our study is justified.Актуальність. На даний час одним з найважчих ускладнень гострих запальних захворювань органів черевної порожнини є перитоніт. Мета. Дослідження розвитку ендотеліальної дисфункції при експериментальному перитоніті та порівняння її корекції за допомогою розчину декаметоксину, аміногуадину та L-аргініну. Матеріали і методи. Дослідження проведено на 260 білих щурах репродуктивного віку (3 місяці), маса тва- рин — 180–220 г. Тварини були розподілені на 4 групи. Каловий перитоніт моделювали шляхом уведення 10 % калової суспензії в дозі 0,5 мл на 100 г ваги тварини до черевної порожнини лабораторних тварин пункційним методом. Результати. Виявлено патологічне підвищення показника вазоконстрикції, викликане експериментальним перитонітом. Встановлено, що використання розчину, який складається з декаметоксину (10 мг/50 мл розчину) і натрію гіалуронату, є ефективним способом санації та основою для подальшої корекції ускладнень експериментального калового перитоніту. Використання аміногуадину у поєднанні з санацією черевної по- рожнини розчином декаметоксину проявило позитивний вплив на зниження рівня ендотеліну-1 в умовах змодельованого перитоніту. Поєднання донатора оксиду азоту з розчином декаметоксину та натрію гіалуронату виявилось ефективнішим порівняно з залученням інгібітора індуцибельної синтази оксиду азоту. Висновки. Враховуючи вплив ендогенної інтоксикації на підвищення маркера вазоконстрикції, можна стверджувати, що одним із першочергових факторів нормалізації рівня ендотеліну-1 є вибір ефективного способу санації черевної порожнини, що обґрунтовує вибір розчину декаметоксину (10 мг/50 мл розчину), в умовах нашого дослідження.Актуальность. В настоящее время одним из наиболее тяжелых осложнений острых воспалительных заболеваний органов брюшной полости является перитонит. Цель. Исследование развития эндотелиальной дисфункции при экспериментальном перитоните и сравнение ее коррекции с помощью раствора декаметоксина, аминогуадина и L-аргинина. Материалы и методы. Исследование проведено на 260 белых крысах репродуктивного возраста (3 месяца), масса животных — 180– 220 г. Животные были распределены на 4 группы. Каловый перитонит моделировали путем введения 10 % каловой суспензии в дозе 0,5 мл на 100 г веса животного в брюшную полость лабораторных животных пункционным методом. Результаты. Выявлено патологическое повышение показателя вазоконстрикции, вызванное экспериментальным перитонитом. Установлено, что использование раствора, состоящего из декаметоксина (10 мг/50 мл раствора) и натрия гиалуроната, является эффек- тивным способом санации и основанием для дальнейшей коррекции осложнений экспериментального калового перитонита. Использование аминогуадина в сочетании с санацией брюшной полости раствором декаметоксина оказало положительное влияние на снижение уровня эндотелина-1 в условиях смоделированного перитонита. Сочетание донатора оксида азота с раствором декаметоксина и натрия гиалуроната оказалось более эффективным по сравнению с вовлечением ингибитора индуцибельной синтазы оксида азота. Выводы. Учитывая влияние эндогенной интоксикации на повышение маркера вазоконстрикции, можно утверждать, что одним из первоочередных факторов нормализации уровня эндотелина-1 является выбор эффективного способа санации брюшной полости, обосновывающий выбор раствора декаметоксина (10 мг/50 мл раствора), в условиях нашего исследования

Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis

Gutsulyuk V. G., Savytskyi I. V., Tsypoviaz S. V., Znamerovskyi S. G., Zashchuk R. G. Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis. Journal of Education, Health and Sport. 2022;12(4):341-346. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.04.029 https://apcz.umk.pl/JEHS/article/view/41779 https://zenodo.org/record/7514133 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 23.03.2022. Revised: 04.04.2022. Accepted: 29.04.2022. UDC 616-092:616-036.8 Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis V. G. Gutsulyuk, I. V. Savytskyi, S. V. Tsypoviaz, S. G. Znamerovskyi, R. G. Zashchuk International Academy of Ecology and Medicine, Kyiv, Ukraine Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road4-а/29, tel.+38050-381-21-83, [email protected] Abstract The article presents the results of studying the role of erythrocyte and leukocyte indices of intoxication in the pathogenesis of experimental peritonitis. When studying the role of erythrocyte and leukocyte indices, a unidirectional trend of their change was revealed. An increase in the erythrocyte index of intoxication in the pathogenesis of experimental fecal peritonitis was established, most pronounced on the 10th day of the experiment (the level of EII increased by 107% compared to intact animals). The results of the study of the leukocyte intoxication index indicate a unidirectional trend, as in the case of the erythrocyte intoxication index. On the first day, the leukocyte index of intoxication increased on 225% (р<0.05), on the 4th day – on 256.7% (р<0.05), and on the 10th day – on 260% (р <0.05) compared to intact animals. It has been proven that the leukocyte index of intoxication is a more sensitive marker compared to the erythrocyte index. Keywords: peritonitis; leukocyte index of intoxication; erythrocyte index of intoxication; pathogenesis

Oxidative stress and lipid peroxidation in experimental peritonitis

Peritonitis is one of the most serious complications of pathology of the abdominal organs, which is characterized by a high mortality rate. Violation of the natural mechanisms of detoxification (immune, detoxification functions of the liver and kidneys), which occur in acute peritonitis, due to primary damage to their structure and the development of maladaptation mechanisms, accompanied by endogenous intoxication syndrome. One of the characteristic and aggravating manifestations of the syndrome of endogenous intoxication is the activation of lipid peroxidation (LPO). The aim: study of the dynamics of primary and secondary products of lipid peroxidation in experimental peritonitis. In the first hour simulated fecal peritonitis development in the rats blood revealed an increase in the level of diene conjugates and malondialdehyde (p <0.001 compared with the results of intact animals. In the second stage was detected more pronounced increase in the level of primary and secondary products of lipid peroxidation (p <0.001) as compared with the data of the same hole in the first stage, and compared with the results of group No. 1. On the 3rd day of simulated peritonitis development in rats there is a marked deterioration of the animal condition, which was manifested in lipid peroxidation progression: the diene conjugates level increased at the significance level p <0.001, and the level of malondialdehyde compared to p <0.05 data from the previous stage of the same group