27 research outputs found
Chemical identity and mechanisms of action and formation of a cell growth inhibitory leachable compound from disposable polycarbonate plastic vessels
This presentation reports the chemical identity and mechanisms of action and formation of a single cell growth inhibitory leachable compound from disposable polycarbonate plastic vessels. 18 leachable compounds were separated and enriched from the total extracts of the vessels. Out of these compounds, only one displayed selective inhibition of the growth of a CHO cell line. High resolution MS and NMR suggested that the leachable compound is a dinitrobisphenol A. Chemical synthesis further confirmed that this leachable compound is one of the seven possible dinitrobisphenol A. Cell assays revealed that this leachable compound acted not only as a cell cycle arrest agent without any effect on cell viability, but also as a GPR35 agonist with moderate potency. Examining the manufacturing process of polycarbonate vessels showed that the formation of this leachable compound requires the combination of three critical process parameters, high temperature molding process, gamma-irradiation, and the presence of air. This study highlights that besides the resin materials the manufacturing process also contributes to the formation of cell growth inhibitory leachable compounds
Identification of FIP200 interaction with the TSC1–TSC2 complex and its role in regulation of cell size control
FIP200 (focal adhesion kinase [FAK] family interacting protein of 200 kD) is a newly identified protein that binds to the kinase domain of FAK and inhibits its kinase activity and associated cellular functions. Here, we identify an interaction between FIP200 and the TSC1–TSC2 complex through FIP200 binding to TSC1. We found that association of FIP200 with the TSC1–TSC2 complex correlated with its ability to increase cell size and up-regulate S6 kinase phosphorylation but was not involved in the regulation of cell cycle progression. Conversely, knockdown of endogenous FIP200 by RNA interference reduced S6 kinase phosphorylation and cell size, which required TSC1 but was independent of FAK. Furthermore, overexpression of FIP200 reduced TSC1–TSC2 complex formation, although knockdown of endogenous FIP200 by RNA interference did not affect TSC1–TSC2 complex formation. Lastly, we showed that FIP200 is important in nutrient stimulation-induced, but not energy- or serum-induced, S6 kinase activation. Together, these results suggest a cellular function of FIP200 in the regulation of cell size by interaction with the TSC1–TSC2 complex
Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions.
Human mesenchymal stem cells (HMSCS) possess three properties of great interest for the development of cell therapies and tissue engineering: multilineage differentiation, immunomodulation, and production of trophic factors. Efficient ex vivo expansion of hMSCs is a challenging requirement for large scale production of clinical grade cells. Low-cost, robust, scalable culture methods using chemically defined materials need to be developed to address this need. This study describes the use of a xeno-free synthetic peptide acrylate surface, the Corning® Synthemax® Surface, for culture of hMSCs in serum-free, defined medium. Cell performance on the Corning Synthemax Surface was compared to cells cultured on biological extracellular matrix (ECM) coatings in xeno-free defined medium and in traditional conditions on tissue culture treated (TCT) plastic in fetal bovine serum (FBS) supplemented medium. Our results show successful maintenance of hMSCs on Corning Synthemax Surface for eight passages, with cell expansion rate comparable to cells cultured on ECM and significantly higher than for cells in TCT/FBS condition. Importantly, on the Corning Synthemax Surface, cells maintained elongated, spindle-like morphology, typical hMSC marker profile and in vitro multilineage differentiation potential. We believe the Corning Synthemax Surface, in combination with defined media, provides a complete synthetic, xeno-free, cell culture system for scalable production of hMSCs
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Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate.
Age-related macular degeneration (AMD), a leading cause of blindness, is characterized by the death of the retinal pigmented epithelium (RPE), which is a monolayer posterior to the retina that supports the photoreceptors. Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. However, protocols for RPE derivation using defined conditions free of nonhuman derivatives (xeno-free) are preferred for clinical translation. This avoids exposing AMD patients to animal-derived products, which could incite an immune response. In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. Cells on Synthemax II-SC were compared with cultures grown with xenogeneic and xeno-free control substrates. This report demonstrates that Synthemax II-SC supports long-term culture of H9 and H14 hESC lines and permits efficient differentiation of hESCs into functional RPE. Expression of RPE-specific markers was assessed by flow cytometry, quantitative polymerase chain reaction, and immunocytochemistry, and RPE function was determined by phagocytosis of rod outer segments and secretion of pigment epithelium-derived factor. Both hESCs and hESC-RPE maintained normal karyotypes after long-term culture on Synthemax II-SC. Furthermore, RPE generated on Synthemax II-SC are functional when seeded onto parylene-C scaffolds designed for clinical use. These experiments suggest that Synthemax II-SC is a suitable, defined substrate for hESC culture and the xeno-free derivation of RPE for cellular therapies
Correction: A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells
Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate.
Age-related macular degeneration (AMD), a leading cause of blindness, is characterized by the death of the retinal pigmented epithelium (RPE), which is a monolayer posterior to the retina that supports the photoreceptors. Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. However, protocols for RPE derivation using defined conditions free of nonhuman derivatives (xeno-free) are preferred for clinical translation. This avoids exposing AMD patients to animal-derived products, which could incite an immune response. In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. Cells on Synthemax II-SC were compared with cultures grown with xenogeneic and xeno-free control substrates. This report demonstrates that Synthemax II-SC supports long-term culture of H9 and H14 hESC lines and permits efficient differentiation of hESCs into functional RPE. Expression of RPE-specific markers was assessed by flow cytometry, quantitative polymerase chain reaction, and immunocytochemistry, and RPE function was determined by phagocytosis of rod outer segments and secretion of pigment epithelium-derived factor. Both hESCs and hESC-RPE maintained normal karyotypes after long-term culture on Synthemax II-SC. Furthermore, RPE generated on Synthemax II-SC are functional when seeded onto parylene-C scaffolds designed for clinical use. These experiments suggest that Synthemax II-SC is a suitable, defined substrate for hESC culture and the xeno-free derivation of RPE for cellular therapies
Long-term multipassage culture of hBM-MSCs in different surface/media conditions.
<p>Cells were cultured for 8 sequential passages using the indicated conditions. Seeding density at each passage was 7000 cells/cm<sup>2</sup>. Cumulative cell number (A), doubling time (B), cumulative population doublings (PDs) (C), and % viability (D) across the multiple passages are shown. The error bars in D represent the average +/− STDEV from 7 serial passages. Statistical analyses by an ANOVA followed by the Tukey’s method performed on the doubling time (7 passages) and % viability (8 passages) found that doubling time of TCT/with serum was significantly higher and that the % viability for TCT/with serum was significantly lower compared to the ECM and Synthemax Surface serum-free conditions (p-value = 0.000).</p
Trilineage differentiation of hBM-MSC after long-term culture on Corning® Synthemax® Surface in xeno-free defined medium.
<p>Cells were cultured on Corning Synthemax Surface for 9 passages prior to the induction of adipogenic, osteogenic, and chondrogenic differentiations. (A) Oil Red O staining of lipid droplets after 20 days of adipogenic induction, (B) Alizarin Red staining of calcium phosphate deposits produced by osteocytes after 21 days of osteogenic induction, (C) Alcian Blue staining of proteoglycans synthesized by chondrocytes after 14 days of chondrogenic induction.</p