Effect of dietary phospholipid level and phospholipid : neutral lipid value on the development of sea bass (Dicentrarchus labrax) larvae fed a compound diet

International audienc

Digestive enzyme activity at different developmental stages of blackspot seabream, Pagellus bogaraveo (Brunnich 1768)

Blackspot seabream, Pagellus bogaraveo (Brunnich), has been identified as a potential species to diversify European aquaculture production. Although rearing aspects have been widely investigated, little information exists on the nutritional requirements for this species. The aim of this study was to build up information on the activity of digestive enzymes at certain developmental stages of blackspot seabream in order to understand the nutritional needs of larvae and post larvae. Fish larvae were reared from hatching to 55 days after hatching (dah), and the feeding plan consisted in rotifers (5–35 dah), Artemia naupli (30–35 dah) metanaupli (35–45) and Gemma microdiet (45–55 dah). At 7, 11, 21, 45 and 55 days after hatching (dah), pooled samples of fish larvae were collected for analysis of trypsin, amylase, lipase, alkaline phosphatase and leucine–alanine peptidase activity. Up to 21 dah, the whole larvae body was used for enzymatic analysis, whereas in older larvae only the dissected abdominal cavity was used. Blackspot seabream body dry weight growth was exponential, increasing from 60 μg at 5 dah to 30±9.7 mg at 55 dah. Amylase specific activity decreased significantly during development, exhibiting at 11 dah (0.6 U mg−1 protein) an average value 2.7 times lower than at 7 dah, and remaining stable between 45 and 55 dah (0.7 U mg protein−1). Trypsin specific activity remained constant until 21 dah (between 38 and 44 mU mg protein−1), which could be related to the larvae feeding regime. At later stages of development, lipase-specific activity exhibited a significant increase (P<0.05), being three times higher at 55 dah (8 U mg protein−1) than at 45 dah. The total activity of the studied digestive enzymes increased significantly during larval development (until 21 dah), whereas afterwards only lipase and leucine–alanine peptidase increased significantly between 45 and 55 dah. The pattern of digestive enzymes activity was related to organogenesis and the type of food used at different developmental stages.Publicado

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass

International audienc

Effects of inclusion of yeast and pig blood protein hydrolysates in microdiets on larval quality of gilthead sea bream Sparus aurata

International audienc

Early weaning of seabass larvae, Dicentrarchus labrax: the effect on microbiota, with particular attention to iron supply and exoenzymes

International audienc

Ontogenese, developpement et physiologie digestive chez les larves de poissons

chap. 12National audienc

Ontogeny, development and digestive physiology of fish larvae

chap. 12International audienc

Algal addition in sea bass (Dicentrarchus labrax) larvae rearing : effect on digestive enzymes

National audienc