982 research outputs found
The Insulin Receptor Substrate 1 Associates With Phosphotyrosine Phosphatase Shptp2 In Liver And Muscle Of Rats.
Insulin stimulates the tyrosine kinase activity of its receptor resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate-1 (IRS-1) which, in turn, associates with proteins containing SH2 domains. It has been shown that IRS-1 associates with the tyrosine phosphatase SHPTP2 in cell cultures. While the effect of the IRS-1/SHPTP2 association on insulin signal transduction is not completely known, this association may dephosphorylate IRS-1 and may play a critical role in the mitogenic actions of insulin. However, there is no physiological demonstration of this pathway of insulin action in animal tissues. In the present study we investigated the ability of insulin to induce association between IRS-1 and SHPTP2 in liver and muscle of intact rats, by co-immunoprecipitation with anti-IRS-1 antibody and anti-SHPTP2 antibody. In both tissues there was an increase in IRS-1 association with SHPTP2 after insulin stimulation. This association occurred when IRS-1 had the highest level of tyrosine phosphorylation and the decrease in this association was more rapid than the decrease in IRS-1 phosphorylation levels. The data provide evidence against the participation of SHPTP2 in IRS-1 dephosphorylation in rat tissues, and suggest that the insulin signal transduction pathway in rat tissues is related mainly to the mitogenic effects of the hormone.311409-1
Assessing the forming temperature role on amorphous and polycrystalline HfO2-based 4 kbit RRAM arrays performance
The impact of temperature during the forming operation on the electrical cells performance and the post-programming stability were evaluated in amorphous and polycrystalline HfO2-based arrays. Forming (between − 40 and 150 °C), reset and set (at room temperature) operations were applied using the incremental step pulse with verify algorithm (ISPVA). The improvements achieved on the forming operation in terms of time and voltages reduction do not impact the subsequent reset/set results. ISPVA perturbations in LRS/HRS current distributions are almost negligible after the first reset/set operation. In this study the best improvement in forming operation in terms of yield, voltage values and cell-to-cell variability is achieved in polycrystalline samples at 80 °C
Gain of 20q11.21 in human pluripotent stem cells impairs TGF-β-dependent neuroectodermal commitment
Gain of 20q11.21 is one of the most common recurrent genomic aberrations in human pluripotent stem cells. Although it is known that overexpression of the antiapoptotic gene Bcl-xL confers a survival advantage to the abnormal cells, their differentiation capacity has not been fully investigated. RNA sequencing of mutant and control hESC lines, and a line transgenically overexpressing Bcl-xL, shows that overexpression of Bcl-xL is sufficient to cause most transcriptional changes induced by the gain of 20q11.21. Moreover, the differentially expressed genes in mutant and Bcl-xL overexpressing lines are enriched for genes involved in TGF-beta- and SMAD-mediated signaling, and neuron differentiation. Finally, we show that this altered signaling has a dramatic negative effect on neuroectodermal differentiation, while the cells maintain their ability to differentiate to mesendoderm derivatives. These findings stress the importance of thorough genetic testing of the lines before their use in research or the clinic
Finite-temperature simulations of the scissors mode in Bose-Einstein condensed gases
The dynamics of a trapped Bose-condensed gas at finite temperatures is
described by a generalized Gross-Pitaevskii equation for the condensate order
parameter and a semi-classical kinetic equation for the thermal cloud, solved
using -body simulations. The two components are coupled by mean fields as
well as collisional processes that transfer atoms between the two. We use this
scheme to investigate scissors modes in anisotropic traps as a function of
temperature. Frequency shifts and damping rates of the condensate mode are
extracted, and are found to be in good agreement with recent experiments.Comment: 4 pages, 3 figure
Collective oscillations of a classical gas confined in harmonic traps
Starting from the Boltzmann equation we calculate the frequency and the
damping of the monopole and quadrupole oscillations of a classical gas confined
in an harmonic potential. The collisional term is treated in the relaxation
time approximation and a gaussian ansatz is used for its evaluation. Our
approach provides an explicit description of the transition between the
hydrodynamic and collisionless regimes in both spherical and deformed traps.
The predictions are compared with the results of a numerical simulation.Comment: 6 pages, revtex, 2 figures include
Overcritical Rotation of a Trapped Bose-Einstein Condensate
The rotational motion of an interacting Bose-Einstein condensate confined by
a harmonic trap is investigated by solving the hydrodynamic equations of
superfluids, with the irrotationality constraint for the velocity field. We
point out the occurrence of an overcritical branch where the system can rotate
with angular velocity larger than the oscillator frequencies. We show that in
the case of isotropic trapping the system exhibits a bifurcation from an
axisymmetric to a triaxial configuration, as a consequence of the interatomic
forces. The dynamical stability of the rotational motion with respect to the
dipole and quadrupole oscillations is explicitly discussed.Comment: 6 pages, 3 postscript figure
Detection of vorticity in Bose-Einstein condensed gases by matter-wave interference
A phase-slip in the fringes of an interference pattern is an unmistakable
characteristic of vorticity. We show dramatic two-dimensional simulations of
interference between expanding condensate clouds with and without vorticity. In
this way, vortices may be detected even when the core itself cannot be
resolved.Comment: 3 pages, RevTeX, plus 6 PostScript figure
Coherent Tunneling of Atoms from Bose-condensed Gases at Finite Temperatures
Tunneling of atoms between two trapped Bose-condensed gases at finite
temperatures is explored using a many-body linear response tunneling formalism
similar to that used in superconductors. To lowest order, the tunneling
currents can be expressed quite generally in terms of the single-particle
Green's functions of the isolated Bose gases. A coherent first-order tunneling
Josephson current between two atomic Bose-condensates is found, in addition to
coherent and dissipative contributions from second-order
condensate-noncondensate and noncondensate-noncondensate tunneling. Our work is
a generalization of Meier and Zwerger, who recently treated tunneling between
uniform atomic Bose gases. We apply our formalism to the analysis of an
out-coupling experiment induced by light wave fields, using a simple
Bogoliubov-Popov quasiparticle approximation for the trapped Bose gas. For
tunneling into the vacuum, we recover the results of Japha, Choi, Burnett and
Band, who recently pointed out the usefulness of studying the spectrum of
out-coupled atoms. In particular, we show that the small tunneling current of
noncondensate atoms from a trapped Bose gas has a broad spectrum of energies,
with a characteristic structure associated with the Bogoliubov quasiparticle
u^2 and v^2 amplitudes.Comment: 26 pages, 5 figures, minor changes, to appear in PR
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