The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta®

Current study provides an efficient screening system for transformed plant of Citrullus lanatus cv. Round Dragon harboring bar gene. The untransformed 5-day-old cotyledon explants were cultured on the shoot-inducing media supplemented with Basta® (0.2, 0.5, 1.0, 2.0 and 3.0 mg L-1) and without Basta® for 3 weeks and subcultured on fresh shoot-inducing media with the same media composition for another 3 weeks. The shoot growth on the cotyledon decreased, as the Basta® concentration increased. A complete inhibition of shoot growth was observed on growth medium supplemented with 2.0 and 3.0 mg L-1 of Basta®, respectively. For ex vitro condition, untransformed healthy plant leaves (derived from acclimatized in vitro plantlets) were leaf painted with an aqueous solution of Basta® at the concentration of 0.001, 0.01 and 0.1% (v/v) using writing brush. The sensitivity of untransformed plant tissues were evaluated based on tissue browning and necrosis due to herbicidal damage. Healthy plant leaves subjected to leaf painting assay showed serious necrotic within 3 days at the concentration of 0.1% (v/v) of Basta®. An efficient herbicide Basta® selection mode has been established via in vitro and ex vitro conditions of untransformed Citrullus lanatus cv. Round Dragon

Effect of acoustic excitation on stalled flows over an airfoil

The effect of acoustic excitation on post-stalled flows over an airfoil, i.e., flows that are fully separated from near the leading edge, is investigated. The excitation results in a tendency towards reattachment, which is accompanied by an increased lift and reduced drag, although the flow may still remain fully separated. It is found that with increasing excitation amplitude, the effect becomes more pronounced but shifts to a Strouhal number which is much lower than that expected from linear, inviscid instability of the separated shear layer

Streamwise vorticity generation and mixing enhancement in free jets by delta-tabs

The effect of triangular tabs, placed at the nozzle exit, on the evolution of free jets is investigated. The effect, a large distortion of the jet cross section and a resultant increase in mixing downstream, has been inferred before to be due to a pair of streamwise vortices originating from each tab. In this paper, the generation mechanism of the stream wise vorticity (omega sub x) is considered first. Two sources are postulated. One is the upstream 'pressure hill', produced by the tab, which appears to be the dominant source. Another is due to vortex filaments shed from the sides of the tab and reoriented downstream by the mean shear of the mixing layer. In the case of a 'delta-tab', a triangular tab with its apex leaning downstream, vorticity from the two sources explain the stronger effect in that configuration. Data on the vorticity evolution for the effect of two delta-tabs are presented, up to twelve jet diameters from the exit, which show that the streamwise vortices persist even at the farthest measurement station. The magnitude of omega sub x-maximum decays continually with distance from the nozzle, its ratio to azimuthal vorticity maximum is found to be about 1/5 everywhere. The relative effect of a delta-tab on jets from an axisymmetric nozzle and a 8:1 rectangular nozzle is also studied. The mixing layer distortion is found to be less pronounced in the rectangular case. The jet mixing, as manifested by the mass flux measured at a downstream station, is increased in the axisymmetric jet but it is decreased in the rectangular jet under consideration by the delta-tab

A steadying effect of acoustic excitation on transitory stall

The effect of acoustic excitation on a class of separated flows with a transitional boundary layer at the point of separation is considered. Experimental results on the flow over airfoils, a two-dimensional backward-facing step, and through large angle conical diffusers are presented. In all cases, the separated flow undergoes large amplitude fluctuations, much of the energy being concentrated at unusually low frequencies. In each case, an appropriate high frequency acoustic excitation is found to be effective in reducing the fluctuations substantially. The effective excitation frequency scales on the initial boundary layer thickness and the effect is apparently achieved through acoustic tripping of the separating boundary layer

Experimental study of boundary layer transition on a heated flat plate

A detailed investigation to the document momentum and thermal development of boundary layers undergoing natural transition on a heated flat plate was performed. Experimental results of both overall and conditionally sampled characteristics of laminar, transitional, and low Reynolds number turbulent boundary layers are presented. Measurements were done in a low-speed, closed-loop wind tunnel with a freestream velocity of 100 ft/s and zero pressure gradient over a range of freestream turbulence intensities from 0.4 to 6 percent. The distributions of skin friction, heat transfer rate, and Reynolds shear stress were all consistent with previously published data. Reynolds analogy factors for momentum thickness Reynolds number, Re(sub theta) less than 2300 were found to be well predicted by laminar and turbulent correlations which accounted for an unheated starting length and uniform heat flux. A small dependence of turbulence results on the freestream turbulence intensity was observed

Acoustics-turbulence interaction

An investigation of the instability frequency was undertaken. Measurements revealed that the hot wire probe induces and sustains stable upstream oscillation of the free shear layer. The characteristics of the free shear layer tone are found to be different from the slit jet wedge edgetone phenomenon. The shear tone induced by a plane wedge in a plane free shear layer was then examined in order to further document the phenomenon. The eigenvalues and eigenfunctions of the tone fundamental show agreement with the spatial stability theory. A comprehensive summary of the results is also included

Turbulent heat flux measurements in a transitional boundary layer

During an experimental investigation of the transitional boundary layer over a heated flat plate, an unexpected result was encountered for the turbulent heat flux (bar-v't'). This quantity, representing the correlation between the fluctuating normal velocity and the temperature, was measured to be negative near the wall under certain conditions. The result was unexpected as it implied a counter-gradient heat transfer by the turbulent fluctuations. Possible reasons for this anomalous result were further investigated. The possible causes considered for this negative bar-v't' were: (1) plausible measurement error and peculiarity of the flow facility, (2) large probe size effect, (3) 'streaky structure' in the near wall boundary layer, and (4) contributions from other terms usually assumed negligible in the energy equation including the Reynolds heat flux in the streamwise direction (bar-u't'). Even though the energy balance has remained inconclusive, none of the items (1) to (3) appear to be contributing directly to the anomaly