the redox sensitive ape1 is a master cellular regulator for inflammatory pain condition

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EFFECT OF SOME NUTRIENTS ON GROWTH, YIELD AND FIBER QUALITY OF EGYPTIAN COTTON UNDER SALINE CONDITION

Two pot experiments were carried out at the greenhouse of the Cotton Research Institute, Agric. Res. Cent., Giza, Egypt during 2015 and 2016 seasons to study the response of cotton plant to application of some nutrients to improve the performance of cotton plant under irrigation of saline water to increase growth, yield and yield components and fiber quality of Giza 90 cotton cultivar.  The experimental design was a split plot design with four replications. Main plots included saline water solutions treatments (control, 2000, 4000, 6000 and 8000 ppm), sub plot included four nutrients application (Potassium Humate, Algex, Poly Ethylene Glycol (PEG) 6000 and Potassein compared with control). The obtained results could be summarized as follows: Irrigation with saline  water over seasons significantly decreased plant height, number of fruiting branches/plant, number of open bolls /plant, boll weight, seed index, seed cotton yield per plant, fiber length, uniformity index, fiber strength and micronaire value,  while, lint % was significantly increased. Nutrients application had significant effect on growth parameters, yield and its components and fiber properties under study, All nutrients treatments gave the highest values of growth parameters, yield and its components and fiber properties as compared with the control in both seasons. In general, plants sprayed with PEG gave the highest averages of plant height, number of fruiting branches/plant. Potassium Humate gave  the highest averages of yield and its components and fiber properties followed by plants sprayed with Algex, while the plants sprayed with PEG as came the last in these respect in both seasons. Interaction between irrigation with slain water and nutrients application by salinity tolerance inducers had a significant effect on plant height, number of fruiting branches per plant,  boll weight,  number of open bolls /plant, seed index, seed cotton yield /plant and fiber quality under study in both seasons. Plants treated with foliar nutrients under salinity condation scored the highest average of growth parameters, yield and its components and fiber properties. Potassium Humate, Algex, PEG and Potassein applications to plants under normal and salinity conditions had positive effects on improving the performance of cotton plants, which increased plant growth and yield especially under salinity conditions

Neurogenesis and growth factors expression after complete spinal cord transection in Pleurodeles waltlii

Following spinal lesion, connections between the supra-spinal centers and spinal neuronal networks can be disturbed, which causes the deterioration or even the complete absence of sublesional locomotor activity. In Mammals, possibilities of locomotion restoration are much reduced since descending tracts either have very poor regenerative ability or do not regenerate at all. However, in lower Vertebrates, there is spontaneous locomotion recuperation after complete spinal cord transection at the mid-trunk level. This phenomenon depends on a translesional descending axon re-growth originating from the brainstem. On the other hand, cellular and molecular mechanisms underlying spinal cord regeneration and in parallel, locomotion restoration of the animal, are not well known. FGF-2 plays an important role in different processes such as neural induction, neuronal progenitor proliferation and their differentiation. Studies had shown an over expression of this growth factor after tail amputation. Nestin, a protein specific for intermediate filaments, is considered as an early marker for neuronal precursors. It has been recently shown that its expression increases after tail transection in Urodeles. Using this marker and western blots, our results show that the increase in the number of FGF-2 and FGFR2 mRNAs is correlated with an increase in neurogenesis especially in the central canal lining cells immediately after lesion. This study also confirms that spinal cord re-growth through the lesion site initially follows a rostrocaudal direction. In addition to its role known in neuronal differentiation, FGF-2 could be implicated in the differentiation of ependymal cells into neuronal progenitors

Synergistic Effect of Celecoxib on 5-fluorouracil-induced Apoptosis in Hepatocellular Carcinoma Patients

Background. Cyclooxygenase-2 (COX-2) enzyme over expression is reported in many human HCC cell line studies and is linked to tumor cell resistance to chemotherapy-induced apoptosis. We hypothesized that adding a COX-2 inhibitor would improve the therapeutic benefits in patients with HCC. COX-2 is often increased and involved in drug resistance and poor prognosis.Method. Between January 2001 and December 2007, 15 patients with MDR-positive-HCC from 34 HCC patients based on tissue and serum liver of glypican-3 and fitting the preset eligibility criteria, were treated with a combination regimen with intravenous infusion of (5-FU) 750 mg once per week, 100mg/day cyclophosphamide (Endoxan) and 400 mg/day celecoxib taken orally in divided doses, while the rest of the patients received only 5-FU and Endoxan. Twenty-one patients (62%) had liver disease associated with hepatitis C virus (HCV) and 5 patients with hepatitis B virus (62%). Results. We found that celecoxib reduced P-glycoprotein with activation of caspase-3 and marked regression of tumor sizes. Sera angiogenic factors (VEGF &amp; bFGF) levels measurement in HCC patients indicated that, the sera levels of both angiogenic factors were reduced significantly (p < 0.05) after treatment. Based on the tumor markers AFP &amp; Glypican-3, 11 of the patients had a PR (11/15), including 3 patients who had normalization of AFP, and four patients had CR (4/15).Conclusions. These data suggest that the combination of 5-FU, Endoxan and Celecoxib is highly effective palliative regimen for patients with HCC with good performance status (score ≤ 3). The study suggests a framework for Celecoxib-based combination treatment of HCC

Alteration of AP-endonuclease1 expression in curcumin-treated fibrotic rats

Background. Apurinic/apyrimidinic endonuclease1/ redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA base excision repair and redox regulation of many transcription factors. It is an important pro-survival protein activated in response to oxidative stress. Increased level of this essential redox sensi¬tive protein correlates closely with cellular survival against oxidative insults. Curcumin (diferuloylmethane) a naturally occurring compound derived from turmeric has attracted interest because of its anti-inflamma¬tory, anti-oxidative, and chemopreventive activities.Material and methods. The current study evaluates the in vivo role of curcumin in protecting and treating liver injury and fibrogenesis caused by carbon tetrachloride (CCl4) in rats. It also addresses the possible involvement of the multifunctional protein APE1 in hepatoprotection. Analysis of APE1 expression was performed at mRNA and protein levels by reverse trans¬criptase (RT)-PCR and western blotting respectively. Profile of HSCs-activation related genes were assayed by RT-PCR and pro-inflammatory cytokines levels were determined by enzyme-linked immune assays.Results. Here we show that oral administration of curcumin was accompanied by a robust increase in APE1 protein and mRNA levels, and improved the histological architecture of rat liver. In addition, curcumin attenuated oxidative stress by increasing the content of hepatic glutathione within normal values, leading to the re¬duction in the level of lipid hydroperoxide. Curcumin remarkably suppressed inflammation by reducing le¬vels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-kB) and interleukin-6 (IL-6). It also inhibited hepatic stellate cells (HSCs) activation by elevating the level of PPARγ and reducing the abundance of transforming growth factor-β (TGF-β). We found that oral adminis¬tration of curcumin at 200 mg/kg dose not only protected against CCl4-induced hepatic injury, but also re¬sulted in more than two-fold induction of APE1 protein expression in CCl4-induced rat group.Conclusions. It can be concluded that curcumin reduced markers of liver damage in rats treated with CCl4, with conco¬mitant elevation in APE1 protein level indicating a possible protective effect with unknown mechanism. The induction of DNA repair enzymes may be an important and novel strategy for hepatic protection against oxidative injury

Regulation of the human AP-endonuclease

(APE1/Ref-1) expression by the tumor suppressor p53 in response to DNA damag