3,375 research outputs found
The differential effects of high-fat and high- -fructose diets on the liver of male albino rat and the proposed underlying mechanisms
Background: The Western-style diet is characterised by the high intake of energy- -dense foods. Consumption of either high-fructose diet or saturated fat resulted in the development of metabolic syndrome. Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome. Many researchers studied the effect of high-fat diet (HFD), high-fructose diet (HFruD) and high-fructose high-fat diet (HFHF) on the liver. The missing data are the comparison effect of these groups i.e. are effects of the HFHF diet on the liver more pronounced? So, this study was designed to compare the metabolic and histopathological effect of the HFD, HFruD, and HFHF on the liver. The proposed underlying mechanisms involved in these changes were also studied.Â
Materials and methods: Twenty four rats were divided into four groups: con- trol, HFD, HFruD, and HFHF. Food was offered for 6 weeks. Biochemical, light microscopic, immunohistochemical (Inducible nitric oxide synthase [iNOS] and alpha-smooth muscle actin [α-SMA]), real-time polymerase chain reaction (gene expression of TNF-α, interleukin-6, Bax, BCL-2, and caspase 3), histomorphometric analysis and oxidative/antioxidative markers (thiobarbituric acid reactive substances [TBARS], malondialdehyde [MDA]/glutathione [GSH] and superoxide dismutase [SOD]) were done.Â
Results: The HFD, HFruD and HFHF groups developed a cluster of liver disorders; steatosis, necrosis, inflammation, apoptosis, ballooning degeneration and cytopla- smic vacuolations. Internal metabolic impairments include elevated serum levels of glucose, triglycerides, low density lipoprotein and decreased serum levels of high density lipoprotein and albumin. The immunoreaction of the α-SMA and iNOS was strong in these groups. The oxidant markers (MDA and TBARS) were elevated, while the antioxidant markers (SOD and GSH) were decreased. The area per cent of collagen, inflammatory markers, caspase 3 and Bax were elevated, while the BCL-2/Bax ratio was decreased. The decrease in PAS, antioxidant markers and the elevation of the α-SMA, iNOS, inflammatory and oxidant markers were obvious in the HFHF when compared to that of the other groups.Â
Conclusions: High-fat diet, HFruD, and HFHF developed morphologic hepatic changes ranging from steatosis to necrosis and inflammation, besides the deve- lopment of internal metabolic impairments. The chief factors of hepatic injury were fat accumulation in the hepatocytes, oxidative stress and highly elevated iNOS. Compared to the other groups, HFHF’s effect was more prominent.
Calcium Levels in Ruffle-Ended and Smooth-Ended Maturation Ameloblasts
Scanning electron microscopy was used to distinguish the topographical characteristics of two maturation ameloblast types in freeze-dried blocks of enamel organ tissue. This distinction was based primarily upon the configuration of the distal ends of the ameloblasts and the presence or absence of wide intercellular spaces.
Energy dispersive x-ray spectrometry was applied to compare calcium levels in various regions of tissue identified as constituting either ruffle-ended or smooth ended ameloblasts. Greater levels of calcium were found in the distal ends of the ruffle-ended cells than in their proximal ends. In addition, greater calcium levels were found in the distal ends of the ruffle-ended cells than the distal ends of the smooth-ended cells. The higher calcium levels in ruffle-ended cells correlates with the view that these cells are actively involved in control of movement of calcium to the enamel front
Identifikasi Miskonsepsi Materi Kesetimbangan Kimia Menggunakan Tes Diagnostik Pilihan Ganda Tiga Tingkat (Three-tier Multiple Choice) Pada Peserta Didik Kelas XI Mia SMA Negeri 2 Pekanbaru
The aim of this research were to know misconceptions and the category level of misconceptions in the second year students of SMA Negeri 2 Pekanbaru aboutt concepts of chemical equallibrium. This research took place in SMA Negeri 2 Pekanbaru and the data were collected on April 2017. was all the students of XI MIA class of four classes. The population consisted of 161 students. The sample was all the population. This research was descriptive quantitative. The technique of collecting data used diagnostic test and interview. The result showed that students found misconception in the definition concept of chemical equallibrium with the percentage was 34,78% (category of average), for the concept of heterogen and homogen was 36,335% (average), the concept of dissociation degree was 33,54% (average), the concept of Kc measurement was 43,785% (average), the concept of Kp measurement was 27,55% (poor), the principle concept of Le Chatelier was 36,764% (average), the concept of using chemical equallibrium in world industry was 23,60% (poor)
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