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Author Correction to: Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage
An amendment to this paper has been published and can be accessed via the original article.</p
Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage
BACKGROUND: The main function of telomerase is at the telomeres but under adverse conditions telomerase can bind to internal regions causing deleterious effects as observed in cancer cells. RESULTS: By mapping the global occupancy of the catalytic subunit of telomerase (Est2) in the budding yeast Saccharomyces cerevisiae, we reveal that it binds to multiple guanine-rich genomic loci, which we termed “non-telomeric binding sites” (NTBS). We characterize Est2 binding to NTBS. Contrary to telomeres, Est2 binds to NTBS in G1 and G2 phase independently of Est1 and Est3. The absence of Est1 and Est3 renders telomerase inactive at NTBS. However, upon global DNA damage, Est1 and Est3 join Est2 at NTBS and telomere addition can be observed indicating that Est2 occupancy marks NTBS regions as particular risks for genome stability. CONCLUSIONS: Our results provide a novel model of telomerase regulation in the cell cycle using internal regions as “parking spots” of Est2 but marking them as hotspots for telomere addition. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-021-01167-1
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Author Correction to: Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage (BMC Biology, (2021), 19, 1, (247), 10.1186/s12915-021-01167-1)
An amendment to this paper has been published and can be accessed via the original article
Additional file 9 of Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage
Supplementary Table 4. List of yeast strains used in this study
Additional file 1 of Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage
Additional file 1: Supplementary Table S1. Genomic coordinates of NTBS regions discovered in at least three out of five independent ChIP-chip experiments
Additional file 10 of Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage
Additional file 10: Supplementary Table 5. List of primers used in this study