An optical coherence tomography (OCT)-based air jet indentation system for measuring the mechanical properties of soft tissues

2008-2009 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Growth effects on mixed culture of Dunaliella salina and Phaeodactylum tricornutum under different inoculation densities and nitrogen concentrations

Dunaliella salina and Phaeodactylum tricornutum are two important marine microalgae rich in bioactive substances and other high-value constituents. In this study, growth effects on mixed culture of these two microalgae were studied under different inoculation proportions (10:0, 7:3, 5:5, 3:7, 0:10) and low, medium and high nitrogen concentrations of 1.4, 14 and 140 mg/l, respectively. By evaluating cell density, OD680, biomass, chlorophyll a and protein content in the culture, it was found that colony cell growth of D. salina and P. tricornutum was increased with the increasing of nitrogen concentrations. Additionally, mixed culture of D. salina and P. tricornutum under high and medium nitrogen concentrations increased the growth of cell colonies (especially when the inoculation proportion was 7:3) and chlorophyll a content by as much as 96.7 and 132.8%. Protein content was also increased by 1.3 and 2.8 folds when compared with that obtained with monoculture of D. salina and P. tricornutum. In contrast, when the mixed culture was done under low concentration of nitrogen, cell colonies growth was restricted due to limitation of nitrogen.Key words: Dunaliella salina, Phaeodactylum tricornutum, inoculation density, mixed-culture, nitrogen concentration

Human motion tracking based on complementary Kalman filter

Miniaturized Inertial Measurement Unit (IMU) has been widely used in many motion capturing applications. In order to overcome stability and noise problems of IMU, a lot of efforts have been made to develop appropriate data fusion method to obtain reliable orientation estimation from IMU data. This article presents a method which models the errors of orientation, gyroscope bias and magnetic disturbance, and compensate the errors of state variables with complementary Kalman filter in a body motion capture system. Experimental results have shown that the proposed method significantly reduces the accumulative orientation estimation errors

A quantitative trait locus for the number of days from sowing to seedling emergence in maize

Quantitative trait locus (QTL) mapping provides useful information for breeding programs since it allows the estimation of genomic locations and genetic effects of chromosomal regions related to the expression of quantitative traits. The number of days from sowing to seedling emergence (NDSSE) is an important agronomic trait in a maize (Zea mays L.) breeding project which is related to yield. To determine its genetic basis, a recombinant inbred line (RIL) population and two nitrogen (N) regimes were used to detect the QTLs associated with NDSSE; as a result, one QTL was identified under high N regime, on chromosome 9, which could explain 6.20% of phenotypic variance and a decrease of 0.18 of NDSSE due to an additive effect. These results are beneficial for understanding the genetic basis of NDSSE in maize breeding project.Key words: Maize (Zea mays L.), quantitative trait locus, recombinant inbred line, nitrogen

Beat-to-beat ambulatory blood pressure estimation based on random forest

Ambulatory blood pressure is critical in predicting some major cardiovascular events; therefore, cuff-less and noninvasive beat-to-beat ambulatory blood pressure measure-ment is of great significance. Machine-learning methods have shown the potential to derive the relationship between physio-logical signal features and ABP. In this paper, we apply random forest method to systematically explorer the inherent connections between photoplethysmography signal, electrocardiogram signal and ambulatory blood pressure. To archive this goal, 18 features were extracted from PPG and ECG signals. Several models with most significant features as inputs and beat-to-beat ABP as outputs were trained and tested on data from the Multi-Parameter Intelligent Monitoring in Intensive Care II database. Results indicate that compared with the common pulse transit time method, the RF method gives a better performance for one-hour continuous estimation of diastolic blood pressure and systolic blood pressure under both the Association for the Advancement of Medical Instrumentation and British Hyper-tension Society standard

Radiation dose and cancer risk from pediatric CT examinations on 64-slice CT: A phantom study

Objective: To measure the radiation dose from CT scans in an anthropomorphic phantom using a 64-slice MDCT, and to estimate the associated cancer risk. Materials and methods: Organ doses were measured with a 5-year-old phantom and thermoluminescent dosimeters. Four protocols; head CT, thorax CT, abdomen CT and pelvis CT were studied. Cancer risks, in the form of lifetime attributable risk (LAR) of cancer incidence, were estimated by linear extrapolation using the organ radiation doses and the LAR data. Results: The effective doses for head, thorax, abdomen and pelvis CT, were 0.7 mSv, 3.5 mSv, 3.0 mSv, 1.3 mSv respectively. The organs with the highest dose were; for head CT, salivary gland (22.33 mGy); for thorax CT, breast (7.89 mGy); for abdomen CT, colon (6.62 mGy); for pelvis CT, bladder (4.28 mGy). The corresponding LARs for boys and girls were 0.015-0.053% and 0.034-0.155% respectively. The organs with highest LARs were; for head CT, thyroid gland (0.003% for boys, 0.015% for girls); for thorax CT, lung for boys (0.014%) and breast for girls (0.069%); for abdomen CT, colon for boys (0.017%) and lung for girls (0.016%); for pelvis CT, bladder for both boys and girls (0.008%). Conclusion: The effective doses from these common pediatric CT examinations ranged from 0.7 mSv to 3.5 mSv and the associated lifetime cancer risks were found to be up to 0.16%, with some organs of higher radiosensitivity including breast, thyroid gland, colon and lungs. © 2010 Elsevier Ireland Ltd. All rights reserved.postprin

Degradation of Serotonin N-Acetyltransferase, a Circadian Regulator, by the N-end Rule Pathway

Serotonin N-acetyltransferase (AANAT) converts serotonin to N-acetylserotonin (NAS), a distinct biological regulator and the immediate precursor of melatonin, a circulating hormone that influences circadian processes, including sleep. N-terminal sequences of AANAT enzymes vary among vertebrates. Mechanisms that regulate the levels of AANAT are incompletely understood. Previous findings were consistent with the possibility that AANAT may be controlled through its degradation by the N-end rule pathway. By expressing the rat and human AANATs and their mutants not only in mammalian cells but also in the yeast Saccharomyces cerevisiae, and by taking advantage of yeast genetics, we show here that two complementary forms of rat AANAT are targeted for degradation by two complementary branches of the N-end rule pathway. Specifically, the N-terminally acetylated (Nt-acetylated) Ac-AANAT is destroyed through the recognition of its Nt-acetylated N-terminal Met residue by the Ac/N-end rule pathway, whereas the non-Nt-acetylated AANAT is targeted by the Arg/N-end rule pathway, which recognizes the unacetylated N-terminal Met-Leu sequence of rat AANAT. We also show, by constructing lysine-to-arginine mutants of rat AANAT, that its degradation is mediated by polyubiquitylation of its Lys residue(s). Human AANAT, whose N-terminal sequence differs from that of rodent AANATs, is longer-lived than its rat counterpart and appears to be refractory to degradation by the N-end rule pathway. Together, these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences.1141Ysciescopu

Rapid detection of adulterated drugs in herbal dietary supplements by wooden-tip electrospray ionization mass spectrometry

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Magnetism and Charge Dynamics in Iron Pnictides

In a wide variety of materials, such as copper oxides, heavy fermions, organic salts, and the recently discovered iron pnictides, superconductivity is found in close proximity to a magnetically ordered state. The character of the proximate magnetic phase is thus believed to be crucial for understanding the differences between the various families of unconventional superconductors and the mechanism of superconductivity. Unlike the AFM order in cuprates, the nature of the magnetism and of the underlying electronic state in the iron pnictide superconductors is not well understood. Neither density functional theory nor models based on atomic physics and superexchange, account for the small size of the magnetic moment. Many low energy probes such as transport, STM and ARPES measured strong anisotropy of the electronic states akin to the nematic order in a liquid crystal, but there is no consensus on its physical origin, and a three dimensional picture of electronic states and its relations to the optical conductivity in the magnetic state is lacking. Using a first principles approach, we obtained the experimentally observed magnetic moment, optical conductivity, and the anisotropy of the electronic states. The theory connects ARPES, which measures one particle electronic states, optical spectroscopy, probing the particle hole excitations of the solid and neutron scattering which measures the magnetic moment. We predict a manifestation of the anisotropy in the optical conductivity, and we show that the magnetic phase arises from the paramagnetic phase by a large gain of the Hund's rule coupling energy and a smaller loss of kinetic energy, indicating that iron pnictides represent a new class of compounds where the nature of magnetism is intermediate between the spin density wave of almost independent particles, and the antiferromagnetic state of local moments.Comment: 4+ pages with additional one-page supplementary materia

Quasi-radial growth of metal tube on si nanowires template

It is reported in this article that Si nanowires can be employed as a positive template for the controllable electrochemical deposition of noble metal tube. The deposited tube exhibits good crystallinity. Scanning electron microscope and transmission electron microscope characterizations are conducted to reveal the growth process of metal tube, showing that the metal tube grows quasi-radially on the wall of Si nanowire. The quasi-radial growth of metal enables the fabrication of thickness-defined metal tube via changing deposition time. Inner-diameter-defined metal tube is achieved by choosing Si nanowires with desired diameter as a template. Metal tubes with inner diameters ranging from 1 μm to sub-50 nm are fabricated