Cross Breeding and Hybrid Identification of Sulphite-tolerant Hybrids of Saccharomyces uvarum

Yeast species belonging to Saccharomyces have great potential for the wine industry. However, the sulphite tolerance of most S. uvarum strains is quite poor compared with that of the other Saccharomyces strains. Inorder to get new S. uvarum strains with tolerance to sulphite, and also with good fermentation characteristics, 21 candidates were screened from three different crossing combinations of sensitive S. uvarum strains to one sulphitetolerant strain. Ten of these hybrids were sulphite tolerant and contained the FZF1 gene from both parents. Intersimple sequence repeat (ISSR) analysis confirmed their hybrid status, based on six primers that produced 55 clear and reproducible bands, including 32 that were polymorphic. Two hybrids had identical fingerprints, indicating that it was the same clone. Thus, nine different novel sulphite-resistant hybrids of S. uvarum were obtained. The selected hybrid strains fermented very well at 30ºC in Sauvignon Blanc grape juice containing 2 mM of sodium sulphite, with minor differences in fermentation performance. Two strains (namely C13 and C21) performed very similarly to the sulphite-tolerant parent A9 and a commercial S. cerevisiae strain EC1118, and the production offermentation aromas, namely propanol, isobutanol and isoamyl alcohol by C13 was found to be the highest. This is the first report of using hybridisation to breed the sulphite-tolerant S. uvarum strains

Study of f_0(980) and f_0(1500) from B_s \to f_0(980)\pi, f_0(1500)\pi Decays

In this paper, we analyze the scalar mesons $f_0(980)$ and $f_0(1500)$ from the decays $\bar B^0_s \to f_0(980)\pi^0, f_0(1500)\pi^0$ within Perturbative QCD approach. From the leading order calculations, we find that (a) in the allowed mixing angle ranges, the branching ratio of $\bar B^0_s\to f_0(980)\pi^0$ is about $(1.0\sim1.6)\times 10^{-7}$, which is smaller than that of $\bar B^0_s\to f_0(980)K^0$ (the difference is a few times even one order); (b) the decay $\bar B^0_s \to f_0(1500)\pi^0$ is better to distinguish between the lowest lying state or the first excited state for $f_0(1500)$, because the branching ratios for two scenarios have about one-order difference in most of the mixing angle ranges; and (c) the direct CP asymmetries of $\bar B^0_s \to f_0(1500)\pi^0$ for two scenarios also exists great difference. In scenario II, the variation range of the value ${\cal A} ^{dir}_{CP}(\bar B^0_s \to f_0(1500)\pi^0)$ according to the mixing angle is very small, except for the values corresponding to the mixing angles being near $90^\circ$ or $270^\circ$, while the variation range of ${\cal A} ^{dir}_{CP}(\bar B^0_s \to f_0(1500)\pi^0)$ in scenario I is very large. Compared with the future data for the decay $\bar B^0_s \to f_0(1500)\pi^0$, it is ease to determine the nature of the scalar meson $f_0(1500)$.Comment: 16 pages, 3 figures, Revte

Evaluation on the Effects of Deicing Chemicals on Soil and Water Environment

AbstractDeicing chemicals are widely used to clear the pavement snow in the cold areas of China and many other countries in winter, because of their simple operation and excellent snow-melting effect, which leads to more convenience for traffic and low probability of traffic accident in the city, especially in metropolis. However, with the ever-growing application of deicing chemicals, their negative effects on environment such as soil and water system increased dramatically. Based on the related researches in China and abroad, this paper summarized the two categories of deicing chemicals and their working principles, and then provided the unique characteristics of deicing chemicals’ effects on soil and water environment in large cities, which may need considerable quantity of the chemicals to relieve traffic congestion. What's more, main aspects of impacts by deicing chemicals on regional soil and water environment and the fundamental factors related to the aspects above respectively were generalized, in order to propose a conceptual model to evaluate the harmful effects. Moreover, this paper put forward some suggestions for the further research in this field to fulfill the model, and provided a scientific basis for the pollution prevention of deicing chemicals on urban environment

Elastic model scale and material for underwater structure of cross-sea bridge

With the bridge construction gradually from inland to coast and offshore, underwater structure of cross-sea bridge can encounter more complex hydrodynamic action. When elastic model is used to simulate the hydrodynamic response of underwater structure, the model scale and model material is the first problem must be solved. Based on principle of similitude, elastic model should meet the geometric similarity, kinematic similarity and mechanical similarity. Formula derivation gives the scale conditions for elastic model are as follows, geometric scale (Lr) equals elastic modulus scale (Er) and density scale (??r) equals 1. Then, how to find a material with same density and the elastic modulus is 1/ Lr of prototype is the key to make a model

Stomach One-Point Cancer: One Case Report and Literature Review

Gastric cancer is one of the most common cancers and one of themost frequent causes of cancer deaths worldwide. Early detection andaccurate preoperative staging of gastric cancer is essential for planning optimal therapy such as endoscopic mucosal resection or gastric resection and offers the best prognosis. With advanced technology in diagnostic instruments and the mass screening, early gastric cancer has been detected easier. One-point cancer of gastric is a special type of early gastric cancer[1]. Diagnosis of one-point cancer of gastric is important for both the immediate treatment and the prognosis. There is still no consensus on the operation extent and postoperative treatment for patients with one-point cancer of gastric. Learned from previous reports[2-5], we know that existed in the superfi cial layer of the gastric mucosa and the superfi cial ulcer is one of the important characteristics of one point cancer of gastric. Herein, we report a case of one point cancer of gastric with the appearance of a deep infi ltrating ulcer. To the best of our knowledge, no such type of one point cancer of gastric has been reported

Polysaccharides and polyphenols in sea buckthorn leaf tea have synergistic impact on studied colonic strains in vitro and bacteria in vivo

Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confirmed that the growth of both beneficial microbe B. longum subsp. longum (0.695, 0.205: R2, slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R2, slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, –0.165: R2, slope, respectively) growth. In vivo studies also revealed that genome copies of Bifidobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment

Nonperturbative Description of Deep Inelastic Structure Functions in Light-Front QCD

We explore the deep inelastic structure functions of hadrons nonperturbatively in an inverse power expansion of the light-front energy of the probe in the framework of light-front QCD. We arrive at the general expressions for various structure functions as the Fourier transform of matrix elements of different components of bilocal vector and axial vector currents on the light-front in a straightforward manner. The complexities of the structure functions are mainly carried by the multi-parton wave functions of the hadrons, while, the bilocal currents have a dynamically dependent yet simple structure on the light-front in this description. We also present a novel analysis of the power corrections based on light-front power counting which resolves some ambiguities of the conventional twist analysis in deep inelastic processes. Further, the factorization theorem and the scale evolution of the structure functions are presented in this formalism by using old-fashioned light-front time-ordered perturbation theory with multi-parton wave functions. Nonperturbative QCD dynamics underlying the structure functions can be explored in the same framework. Once the nonperturbative multi-parton wave functions are known from low-energy light-front QCD, a complete description of deep inelastic structure functions can be realized.Comment: Revtex, 30 pages and no figur