Relationship between HPV16/18 E6 and р53, р21waf1, MDM2, Ki67 and cyclin D1 expression in esophageal squamous cell carcinoma: comparative study by using tissue microarray technology

Aim: To investigate the role of human papillomavirus (HPV) HPV16/18 E6 oncogene in the carcinogenesis of esophageal cell carcinoma (ESCC). Materials and Methods: Tissue microarray (TMA) block was constructed from 60 cases of paraffin-embedded ESCC tissues and pair-matched controls (adjacent normal epithelium). Immunohistochemistry (IHC) methods were applied to detect the expression of HPV16/18 E6, p53, p21Waf1, MDM2, Ki67 and cyclin D1 proteins on TMA slides. In situ hybridization (ISH) targeting HPV gene was also used. Results: In ESCC samples, 18.3% (11/60) were revealed HPV16/18 E6 positive by IHC, while 40.0% (24/60) HPV positive by ISH; HPV16/18 E6 expression was significantly higher than that of control samples. In ESCC samples, the expressions of p53, p21Waf1, Cyclin D1, MDM2 and Ki67 were recorded in 60.0% (36/60), 40.0% (24/60), 51.7% (31/60), 65.0% (39/60) and 88.3% (53/60) cases respectively, In ESCC samples, p53, MDM2 and Ki67 expression correlated with the HPV16/18 E6 expression (p < 0.01), p21Waf1 expression — with these of MDM2 and cyclin D1 (p < 0.01) whilst expression of Ki67 — with ESCC grade (p < 0.01). Conclusion: HPV might be one of etiological factor of esophageal carcinoma in Shantou, China. p53, MDM2 proteins may play important roles in the pathogenesis of HPV-associated ESCC.Цель: исследовать роль онкогена HPV16/18 E6 вируса папилломы человека (HPV) в развитии плоскоклеточной карциномы пищевода (ESCC). Материалы и методы: исследованы 60 образцов ESCC и контрольные образцы нетрансформированной ткани (парафиновые блоки). Для выявления экспрессии белков HPV16/18 E6, p53, p21Waf1, MDM2, Ki67 и циклина D1 в срезах для тканевых микрочипов (tissue microarray, TMA) применен метод иммуногистохимии (IHC), для определения экспрессии генов HPV применяли гибридизацию in situ (ISH). Результаты: методами IHC, экспрессия HPV16/18 E6 выявлена в 18,3% (11/60) случаев ESCC, в то время как по результатам метода ISH, 40,0% (24/60) образцов признаны HPV-позитивными. В образ­цах ESCC экспрессия p53, p21Waf1, циклина D1, MDM2 и Ki67 выявлена в 60,0% (36/60), 40,0% (24/60), 51,7% (31/60), 65,0% (39/60) и 88,3% (53/60) случаев соответ­ственно, причем экспрессия p53, MDM2 и Ki67 коррелировала с экспрессией HPV16/18 E6 (p < 0,01), экспрессия p21Waf1 — с таковой MDM2 и циклина D1, а экспрессия Ki67 — со стадией развития опухоли (p < 0.01). Выводы: HPV может являться одним из этиологических факторов развития рака пищевода в провинции Шанту, Китай. Белки p53 и MDM2 могут принимать участие в патогенезе плоскоклеточного рака пищевода, ассоциированного с вирусом папилломатоза

Tuning metal-carboxylate coordination in crystalline metal-organic frameworks through surfactant media

10.1016/j.jssc.2013.07.031Journal of Solid State Chemistry20627-31JSSC

A novel spectrophotometric method for the determination of copper ion by using a salophen ligand, N,N'-disalicylidene-2,3-diaminopyridine

WOS: 000411700000016this study, a novel, selective and highly sensitive spectrophotometric method has been developed for the determination of copper(II) by using a new chromogenic reagent N,N'-bissalicylidene-2,3-diaminopyridine (H2IF). The absorbance was measured at lambda max = 414 nm. Experimental conditions were optimized, and Beer's law was observed in the concentration range of 6.35-318 mu g L-1 of copper. The molar absorptivity of the complex was calculated and found to be 1.46 x 10(5) L mol(-1) cm(-1). The detection and quantification limits were calculated and found to be 6.38 mu g L-1 and 21.27 mu g L-1, respectively. The calculated relative standard deviation (n = 5) was 0.62% for a standard solution including 63.5 mu g L-1 copper( II). The interference effects of the common ions have been studied and the statistical assessment of the obtained results is presented. The developed method was applied for the determination of copper in water samples. The reliability and accuracy of the developed method was proven by the analysis of a waste water standard. (C) 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of Taibah University