A blowout jet associated with one obvious extreme-ultraviolet wave and one complicated coronal mass ejection event

In this paper, we present a detailed analysis of a coronal blowout jet eruption which was associated with an obvious extreme-ultraviolet (EUV) wave and one complicated coronal mass ejection (CME) event based on the multi-wavelength and multi-view-angle observations from {\sl Solar Dynamics Observatory} and {\sl Solar Terrestrial Relations Observatory}. It is found that the triggering of the blowout jet was due to the emergence and cancellation of magnetic fluxes on the photosphere. During the rising stage of the jet, the EUV wave appeared just ahead of the jet top, lasting about 4 minutes and at a speed of 458 - \speed{762}. In addition, obvious dark material is observed along the EUV jet body, which confirms the observation of a mini-filament eruption at the jet base in the chromosphere. Interestingly, two distinct but overlapped CME structures can be observed in corona together with the eruption of the blowout jet. One is in narrow jet-shape, while the other one is in bubble-shape. The jet-shaped component was unambiguously related with the outwardly running jet itself, while the bubble-like one might either be produced due to the reconstruction of the high coronal fields or by the internal reconnection during the mini-filament ejection according to the double-CME blowout jet model firstly proposed by Shen et al. (2012b), suggesting more observational evidence should be supplied to clear the current ambiguity based on large samples of blowout jets in future studies.Comment: APJ, Accepted October 19, 201

A new small satellite sunspot triggering recurrent standard- and blowout-coronal jets

In this paper,we report a detailed analysis of recurrent jets originated from a location with emerging, canceling and converging negative magnetic field at the east edge of NOAA active region AR11166 from 2011 March 09 to 10. The event presented several interesting features. First, a satellite sunspot appeared and collided with a pre-existing opposite polarity magnetic field and caused a recurrent solar jet event. Second, the evolution of the jets showed blowout-like nature and standard characteristics. Third, the satellite sunspot exhibited a motion toward southeast of AR11166 and merged with the emerging flux near the opposite polarity sunspot penumbra, which afterward, due to flux convergence and cancellation episodes, caused recurrent jets. Fourth, three of the blowout jets associated with coronal mass ejections (CMEs), were observed from field of view of the Solar Terrestrial Relations Observatory. Fifth, almost all the blowout jet eruptions were accompanied with flares or with more intense brightening in the jet base region, while almost standard jets did not manifest such obvious feature during eruptions. The most important, the blowout jets were inclined to faster and larger scale than the standard jets. The standard jets instead were inclined to relative longer-lasting. The obvious shearing and twisting motions of the magnetic field may be interpreted as due to the shearing and twisting motions for a blowout jet eruption. From the statistical results, about 30% blowout jets directly developed into CMEs. It suggests that the blowout jets and CMEs should have a tight relationship.Comment: ApJ 18 pages, 7 figure

Zika Virus Attenuation by Codon Pair Deoptimization Induces Sterilizing Immunity in Mouse Models.

Zika virus (ZIKV) infection during the large epidemics in the Americas is related to congenital abnormities or fetal demise. To date, there is no vaccine, antiviral drug, or other modality available to prevent or treat Zika virus infection. Here we designed novel live attenuated ZIKV vaccine candidates using a codon pair deoptimization strategy. Three codon pair-deoptimized ZIKVs (Min E, Min NS1, and Min E+NS1) were de novo synthesized and recovered by reverse genetics and contained large amounts of underrepresented codon pairs in the E gene and/or NS1 gene. The amino acid sequence was 100% unchanged. The codon pair-deoptimized variants had decreased replication fitness in Vero cells (Min NS1 ≫ Min E > Min E+NS1), replicated more efficiently in insect cells than in mammalian cells, and demonstrated diminished virulence in a mouse model. In particular, Min E+NS1, the most restrictive variant, induced sterilizing immunity with a robust neutralizing antibody titer, and a single immunization achieved complete protection against lethal challenge and vertical ZIKV transmission during pregnancy. More importantly, due to the numerous synonymous substitutions in the codon pair-deoptimized strains, reversion to wild-type virulence through gradual nucleotide sequence mutations is unlikely. Our results collectively demonstrate that ZIKV can be effectively attenuated by codon pair deoptimization, highlighting the potential of Min E+NS1 as a safe vaccine candidate to prevent ZIKV infections.IMPORTANCE Due to unprecedented epidemics of Zika virus (ZIKV) across the Americas and the unexpected clinical symptoms, including Guillain-Barré syndrome, microcephaly, and other birth defects in humans, there is an urgent need for ZIKV vaccine development. Here we provided the first attenuated versions of ZIKV with two important genes (E and/or NS1) that were subjected to codon pair deoptimization. Compared to parental ZIKV, the codon pair-deoptimized ZIKVs were mammal attenuated and preferred insect to mammalian cells. Min E+NS1, the most restrictive variant, induced sterilizing immunity with a robust neutralizing antibody titer and achieved complete protection against lethal challenge and vertical virus transmission during pregnancy. More importantly, the massive synonymous mutational approach made it impossible for the variant to revert to wild-type virulence. Our results have proven the feasibility of codon pair deoptimization as a strategy to develop live attenuated vaccine candidates against flaviviruses such as ZIKV, Japanese encephalitis virus, and West Nile virus

In vivo imaging of Zika virus reveals dynamics of viral invasion in immune-sheltered tissues and vertical propagation during pregnancy

Rationale: Zika virus (ZIKV) is a pathogenic virus known to cause a wide range of congenital abnormalities, including microcephaly, Guillain-Barre syndrome, meningoencephalitis, and other neurological complications, in humans. This study investigated the noninvasive detection of ZIKV infection in vivo, which is necessary for elucidating the virus's mechanisms of viral replication and pathogenesis, as well as to accelerate the development of anti-ZIKV therapeutic strategies. Methods: In this study, a recombinant ZIKV harbouring Nluc gene (ZIKV-Nluc) was designed, recovered, and purified. The levels of bioluminescence were directly correlated with viral loads in vitro and in vivo. The dynamics of ZIKV infection in A129 (interferon (IFN)-α/β receptor deficient), AG6 (IFN-α/β and IFN-γ receptor deficient), and C57BL/6 mice were characterized. Pregnant dams were infected with ZIKV-Nluc at E10 via intra footpad injection. Then, the pooled immune sera (anti-ZIKV neutralizing antibodies) #22-1 in ZIKV-Nluc virus-infected mice were visualized. Results: ZIKV-Nluc showed a high genetic stability and replicated well in cells with similar properties to the wild-type ZIKV (ZIKVwt). Striking bioluminescence signals were consistently observed in animal organs, including spleen, intestine, testis, uterus/ovary, and kidney. The ileocecal junction was found to be the crucial visceral target. Infection of pregnant dams with ZIKV-Nluc showed that ZIKV was capable of crossing the maternal-fetal barrier to infect the fetuses via vertical transmission. Furthermore, it was visualized that treatment with the pooled immune sera was found to greatly restrict the spread of the ZIKV-Nluc virus in mice. Conclusions: This study is the first to report the real-time noninvasive tracking of the progression of ZIKV invading immune-sheltered tissues and propagating vertically during pregnancy. The results demonstrate that ZIKV-Nluc represents a powerful tool for the study of the replication, dissemination, pathogenesis, and treatment of ZIKV in vitro and in vivo

The implement of plastic oval tags for mark-recapture in juvenile Japanese flounder (Paralichthys olivaceus) on the northeast coast of Shandong Province, China

As part of the stock enhancement research project of Shandong Province, China, plastic oval tags (POTs) were used to mark juvenile Japanese flounder for release, Paralichthys olivaceus (70 to 133 mm total length, TL), in 2009 and 2010. Optimal tag placement locations, retention, tagging rates, and mortality were initially evaluated. Mark–recapture experiments were carried out in the coastal waters of Weihai City to study their migratory movements: 21,202 individuals in July 2009 at Beihai and 18,350 individuals in July 2010 at Lidao. The number of recaptured individuals were 434 (2.05% recapture rate) in 2009 and 620 (3.38% recapture rate) in 2010. A radiative movement from the release site was observed in the 2009 experiment; however, the tagging experiment showed a predominantly northward dispersal of tagged flounder from the release site in 2010. The mean movement speed of the released fish was calculated as 0.46 km day-1 in 2009 and 1.05 km day-1 in 2010. Furthermore, in 2009, the average TL and wet mass increments were 36.3 ± 8.4 mm month-1 and 27.13 ± 16.09 g month-1, respectively 1 to 6 months after releasing; however, the increments were 14.7 ± 8.8 mm month-1 and 5.65 ± 4.17 g month-1, respectively in 2010.Key words: Paralichthys olivaceus, plastic oval tag, mark–recapture, movement, growth

The cumulative effects of known susceptibility variants to predict primary biliary cirrhosis risk.

Multiple genetic variants influence the risk for development of primary biliary cirrhosis (PBC). To explore the cumulative effects of known susceptibility loci on risk, we utilized a weighted genetic risk score (wGRS) to evaluate whether genetic information can predict susceptibility. The wGRS was created using 26 known susceptibility loci and investigated in 1840 UK PBC and 5164 controls. Our data indicate that the wGRS was significantly different between PBC and controls (P=1.61E-142). Moreover, we assessed predictive performance of wGRS on disease status by calculating the area under the receiver operator characteristic curve. The area under curve for the purely genetic model was 0.72 and for gender plus genetic model was 0.82, with confidence limits substantially above random predictions. The risk of PBC using logistic regression was estimated after dividing individuals into quartiles. Individuals in the highest disclosed risk group demonstrated a substantially increased risk for PBC compared with the lowest risk group (odds ratio: 9.3, P=1.91E-084). Finally, we validated our findings in an analysis of an Italian PBC cohort. Our data suggested that the wGRS, utilizing genetic variants, was significantly associated with increased risk for PBC with consistent discriminant ability. Our study is a first step toward risk prediction for PBC

Neutron Scattering Measurements of Spatially Anisotropic Magnetic Exchange Interactions in Semiconducting K0.85Fe1.54Se2 (TN=280 K)

We use neutron scattering to study the spin excitations associated with the stripe antiferromagnetic (AFM) order in semiconducting K$_{0.85}$Fe$_{1.54}$Se$_2$ ($T_N$=$280$ K). We show that the spin wave spectra can be accurately described by an effective Heisenberg Hamiltonian with highly anisotropic in-plane couplings at $T$= $5$ K. At high temperature ($T$= $300$ K) above $T_N$, short range magnetic correlation with anisotropic correlation lengths are observed. Our results suggest that, despite the dramatic difference in the Fermi surface topology, the in-plane anisotropic magnetic couplings are a fundamental property of the iron based compounds; this implies that their antiferromagnetism may originate from local strong correlation effects rather than weak coupling Fermi surface nesting.Comment: 5 pages, 4 figure