449 research outputs found
Analysis of the distribution and structure of integrated banana streak virus DNA in a range of Musa cultivars
The cDNA encoding the glycoprotein α (GPα) subunit of tilapia (Oreochromis mossambicus) was partially cloned using RACE-polymerase chain reaction (PCR) technique. The amplified cDNA was found to be 583 bases long, and to consist of a portion of the signal peptide, the full sequence encoding the mature peptide (94 amino acids) and the 3′ untranslated region. Northern blot analysis revealed a single band of approximately 600 bp. Alignment of the deduced amino acids of the mature protein showed that the tilapia GPα subunit shares more than 80% identity with that of other perciform fish (i.e. striped bass, sea bream and yellowfin porgy) and less than 70% with that of more taxonomically remote fish and other vertebrates. Exposure of dispersed tilapia pituitary cells to salmon gonadotropin-releasing hormone (sGnRH) elevated GPα mRNA levels via both PKC and cAMP-protein kinase A (PKA) pathways. The transcript levels were also regulated by pituitary adenylate cyclase activating polypeptide (PACAP) and neuropeptide Y (NPY), both acting through PKC and PKA pathways. Moreover, a combined treatment of PACAP or NPY with GnRH seems to have an additive effect on the GPα subunit gene transcription. These results suggest that in tilapia the expression of GPα subunit is regulated by GnRH mainly via PKC and PKA pathways. Furthermore, PACAP and NPY can elevate the GnRH-stimulated GPα subunit transcription and can directly affect the subunit mRNA levels, via the same transduction pathways
Equilibration and Dynamic Phase Transitions of a Driven Vortex Lattice
We report on the observation of two types of current driven transitions in
metastable vortex lattices. The metastable states, which are missed in usual
slow transport measurements, are detected with a fast transport technique in
the vortex lattice of undoped
2H-NbSe. The transitions are seen by following the evolution of these
states when driven by a current. At low currents we observe an equilibration
transition from a metastable to a stable state, followed by a dynamic
crystallization transition at high currents.Comment: 5 pages, 4 figure
The In Vivo Kinetics of RNA Polymerase II Elongation during Co-Transcriptional Splicing
Kinetic analysis shows that RNA polymerase elongation kinetics are not modulated by co-transcriptional splicing and that post-transcriptional splicing can proceed at the site of transcription without the presence of the polymerase
Edge and Bulk Transport in the Mixed State of a Type-II Superconductor
By comparing the voltage-current (V-I) curves obtained before and after
cutting a sample of 2H-NbSe2, we separate the bulk and edge contributions to
the transport current at various dissipation levels and derive their respective
V- I curves and critical currents. We find that the edge contribution is
thermally activated across a current dependent surface barrier. By contrast the
bulk V-I curves are linear, as expected from the free flux flow model. The
relative importance of bulk and edge contributions is found to depend on
dissipation level and sample dimensions. We further show that the peak effect
is a sharp bulk phenomenon and that it is broadened by the edge contribution
Theory of nonlinear optical properties of phenyl-substituted polyacetylenes
In this paper we present a theoretical study of the third-order nonlinear
optical properties of poly(diphenyl)polyacetylene (PDPA) pertaining to the
third-harmonic-generation (THG) process. We study the aforesaid process in
PDPA's using both the independent electron Hueckel model, as well as
correlated-electron Pariser-Parr-Pople (P-P-P) model. The P-P-P model based
calculations were performed using various configuration interaction (CI)
methods such as the the multi-reference-singles-doubles CI (MRSDCI), and the
quadruples-CI (QCI) methods, and the both longitudinal and the transverse
components of third-order susceptibilities were computed. The Hueckel model
calculations were performed on oligo-PDPA's containing up to fifty repeat
units, while correlated calculations were performed for oligomers containing up
to ten unit cells. At all levels of theory, the material exhibits highly
anisotropic nonlinear optical response, in keeping with its structural
anisotropy. We argue that the aforesaid anisotropy can be divided over two
natural energy scales: (a) the low-energy response is predominantly
longitudinal and is qualitatively similar to that of polyenes, while (b) the
high-energy response is mainly transverse, and is qualitatively similar to that
of trans-stilbene.Comment: 13 pages, 7 figures (included), to appear in Physical Review B (April
15, 2004
Focusing and Compression of Ultrashort Pulses through Scattering Media
Light scattering in inhomogeneous media induces wavefront distortions which
pose an inherent limitation in many optical applications. Examples range from
microscopy and nanosurgery to astronomy. In recent years, ongoing efforts have
made the correction of spatial distortions possible by wavefront shaping
techniques. However, when ultrashort pulses are employed scattering induces
temporal distortions which hinder their use in nonlinear processes such as in
multiphoton microscopy and quantum control experiments. Here we show that
correction of both spatial and temporal distortions can be attained by
manipulating only the spatial degrees of freedom of the incident wavefront.
Moreover, by optimizing a nonlinear signal the refocused pulse can be shorter
than the input pulse. We demonstrate focusing of 100fs pulses through a 1mm
thick brain tissue, and 1000-fold enhancement of a localized two-photon
fluorescence signal. Our results open up new possibilities for optical
manipulation and nonlinear imaging in scattering media
Magnetic skyrmion lattices in heavy fermion superconductor UPt3
Topological analysis of nearly SO(3)_{spin} symmetric Ginzburg--Landau
theory, proposed for UPt by Machida et al, shows that there exists a new
class of solutions carrying two units of magnetic flux: the magnetic skyrmion.
These solutions do not have singular core like Abrikosov vortices and at low
magnetic fields they become lighter for strongly type II superconductors.
Magnetic skyrmions repel each other as at distances much larger then the
magnetic penetration depth , forming a relatively robust triangular
lattice. The magnetic induction near is found to increase as
. This behavior agrees well with experiments.Comment: 4 pages, 2 figures, 2 column format; v2:misprint in the title is
correcte
Electron-lattice relaxation, and soliton structures and their interactions in polyenes
Density matrix renormalisation group calculations of a suitably parametrised
model of long polyenes (polyacetylene oligomers), which incorporates both long
range Coulomb interactions and adiabatic lattice relaxation, are presented. The
triplet and 2Ag states are found to have a 2-soliton and 4-soliton form,
respectively, both with large relaxation energies. The 1Bu state forms an
exciton-polaron and has a very small relaxation energy. The relaxed energy of
the 2Ag state lies below that of the 1Bu state. The soliton/anti-soliton pairs
are bound.Comment: RevTeX, 5 pages, 4 eps figures included using epsf. To appear in
Physical Review Letters. Fig. 1 fixed u
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