Changing the bonding force of impression tray to edentulous maxillary jaw simulator with impression valve system: In vitro study

Objective: The aim of this study was to evaluate the effect of an impression valve system (IVS) on the bonding force between an  impression tray and an edentulous maxillary jaw.Materials and Methods: In this in vitro study, a polyether.coated maxillary jaw simulator (PM) was used to model an edentulous maxillary jaw. The IVS was placed into individual impression trays. An irreversible hydrocolloid impression was taken of the PM when the IVS was open and closed. The impression tray bonding force was measured using a digital dynamometer. Student's t.test was used to determine the significance of the difference between these two groups.Results: The impression tray was more easily separated from the PM when the IVS was open (108 ± 3.9 N). The separation was more difficult when the IVS was closed (153.7 ±14.2 N). The difference between these two findings (P = 0.000) was significant.Conclusion: The use of an IVS facilitates the removal of the impression tray from the mouth when taking impressions from an edentulous maxillary jaw.Key words: Bonding force, impression tray, maxillary ja

Regulation of fibroblast growth factor receptor signalling and trafficking by Src and Eps8

Fibroblast growth factor receptors (FGFRs) mediate a wide spectrum of cellular responses that are crucial for development and wound healing. However, aberrant FGFR activity leads to cancer. Activated growth factor receptors undergo stimulated endocytosis, but can continue to signal along the endocytic pathway. Endocytic trafficking controls the duration and intensity of signalling, and growth factor receptor signalling can lead to modifications of trafficking pathways. We have developed live-cell imaging methods for studying FGFR dynamics to investigate mechanisms that coordinate the interplay between receptor trafficking and signal transduction. Activated FGFR enters the cell following recruitment to pre-formed clathrin-coated pits (CCPs). However, FGFR activation stimulates clathrin-mediated endocytosis; FGF treatment increases the number of CCPs, including those undergoing endocytosis, and this effect is mediated by Src and its phosphorylation target Eps8. Eps8 interacts with the clathrin-mediated endocytosis machinery and depletion of Eps8 inhibits FGFR trafficking and immediate Erk signalling. Once internalized, FGFR passes through peripheral early endosomes en route to recycling and degredative compartments, through an Src- and Eps8-dependent mechanism. Thus Eps8 functions as a key coordinator in the interplay between FGFR signalling and trafficking. This work provides the first detailed mechanistic analysis of growth factor receptor clustering at the cell surface through signal transduction and endocytic trafficking. As we have characterised the Src target Eps8 as a key regulator of FGFR signalling and trafficking, and identified the early endocytic system as the site of Eps8-mediated effects, this work provides novel mechanistic insight into the reciprocal regulation of growth factor receptor signalling and trafficking

Extending and validating a human papillomavirus (HPV) knowledge measure in a national sample of Canadian parents of boys

As the human papillomavirus (HPV) vaccine is now recommended for males, a reliable, comprehensive HPV knowledge measurement tool which addresses issues relevant to males is needed. We aimed to replicate, validate and test the comprehensiveness of an existing general HPV and an HPV vaccination knowledge scale in English and French. We also measured parental HPV knowledge and changes over time. An online questionnaire was administered in February (Time 1; T1) and November 2014 (Time 2; T2) to a nationally representative sample of Canadian parents of boys. Dimensionality, internal consistency and model fit were evaluated at both time points and separately in English and French sub-samples. Differences in knowledge scores were measured. Analyses were performed on 3117 participants at T1 and 1427 at T2. The 25-item HPV general knowledge and an 11-item HPV vaccination scale were unidimensional, showed high internal consistency (α>0.87, α>0.73) and had good model fit. Both general HPV and vaccine-specific knowledge significantly increased over time in both languages, but remained low at T2, with only about half of the items being answered correctly. Correct responses at T2 are best explained by correct responses at T1, with some small changes from 'Don't know' at T1 to correct at T2. The extended general and vaccine-specific knowledge scales are valid, reliable and comprehensive, and could be used among parents of boys, in both English and French. Educational interventions could target specific knowledge gaps and focus on providing information rather than correcting misconceptions

Are Canadian Women Prepared for the Transition to Primary HPV Testing in Cervical Screening? A National Survey of Knowledge, Attitudes, and Beliefs

As Canadian provinces and territories prepare to transition to HPV-based primary screening for cervical cancer, failure to identify and address potential barriers to screening could hinder program implementation. We examined screening-eligible Canadians' attitudes towards and knowledge of cervical screening. A nationally representative sample of screening-eligible Canadians (N = 3724) completed a web-based survey in the summer of 2022. Oversampling ensured that half of the sample were underscreened for cervical cancer (>3 years since previous screening or never screened). The participants completed validated scales of cervical cancer, HPV, and HPV test knowledge and HPV test and self-sampling attitudes and beliefs. Between-group differences (underscreened vs. adequately screened) were calculated for scales and items using independent sample t-tests or chi-square tests. The underscreened participants (n = 1871) demonstrated significantly lower knowledge of cervical cancer, HPV, and the HPV test. The adequately screened participants (n = 1853) scored higher on the Confidence and Worries subscales of the HPV Test Attitudes and Beliefs Scale. The underscreened participants scored higher on the Personal Barriers and Social Norms subscales. The underscreened participants also endorsed greater Autonomy conferred by self-sampling. Our findings suggest important differential patterns of knowledge, attitudes, and beliefs between the underscreened and adequately screened Canadians. These findings highlight the need to develop targeted communication strategies and promote patient-centered, tailored approaches in cervical screening programs

Guidelines and best practices for assessing young children remotely

Given the recent rise in the use of remote assessments to collect data from young children, researchers and practitioners would benefit from guidance on best practices within the field. Based on our experiences with assessing over 600 preschoolers remotely, our research team provides a set of main principles to guide professionals to successfully create and operationalize systems for remote assessment. Guidelines include detailed information about how to choose a technology platform, select and use online assessments, and how to adapt traditional tasks for remote use. We also note the challenges inherent in using certain types of tasks, provide tips for scheduling remote sessions, and offer advice for how to promote children’s engagement throughout the assessment process

Performance of the ARIANNA Hexagonal Radio Array

Installation of the ARIANNA Hexagonal Radio Array (HRA) on the Ross Ice Shelf of Antarctica has been completed. This detector serves as a pilot program to the ARIANNA neutrino telescope, which aims to measure the diffuse flux of very high energy neutrinos by observing the radio pulse generated by neutrino-induced charged particle showers in the ice. All HRA stations ran reliably and took data during the entire 2014-2015 austral summer season. A new radio signal direction reconstruction procedure is described, and is observed to have a resolution better than a degree. The reconstruction is used in a preliminary search for potential neutrino candidate events in the data from one of the newly installed detector stations. Three cuts are used to separate radio backgrounds from neutrino signals. The cuts are found to filter out all data recorded by the station during the season while preserving 85.4% of simulated neutrino events that trigger the station. This efficiency is similar to that found in analyses of previous HRA data taking seasons.Comment: Proceedings from the 34th ICRC2015, http://icrc2015.nl/ . 8 pages, 6 figure

A First Search for Cosmogenic Neutrinos with the ARIANNA Hexagonal Radio Array

The ARIANNA experiment seeks to observe the diffuse flux of neutrinos in the 10^8 - 10^10 GeV energy range using a grid of radio detectors at the surface of the Ross Ice Shelf of Antarctica. The detector measures the coherent Cherenkov radiation produced at radio frequencies, from about 100 MHz to 1 GHz, by charged particle showers generated by neutrino interactions in the ice. The ARIANNA Hexagonal Radio Array (HRA) is being constructed as a prototype for the full array. During the 2013-14 austral summer, three HRA stations collected radio data which was wirelessly transmitted off site in nearly real-time. The performance of these stations is described and a simple analysis to search for neutrino signals is presented. The analysis employs a set of three cuts that reject background triggers while preserving 90% of simulated cosmogenic neutrino triggers. No neutrino candidates are found in the data and a model-independent 90% confidence level Neyman upper limit is placed on the all flavor neutrino+antineutrino flux in a sliding decade-wide energy bin. The limit reaches a minimum of 1.9x10^-23 GeV^-1 cm^-2 s^-1 sr^-1 in the 10^8.5 - 10^9.5 GeV energy bin. Simulations of the performance of the full detector are also described. The sensitivity of the full ARIANNA experiment is presented and compared with current neutrino flux models.Comment: 22 pages, 22 figures. Published in Astroparticle Physic

Radar absorption, basal reflection, thickness and polarization measurements from the Ross Ice Shelf, Antarctica

Radio-glaciological parameters from the Moore’s Bay region of the Ross Ice Shelf, Antarctica, have been measured. The thickness of the ice shelf in Moore’s Bay was measured from reflection times of radio-frequency pulses propagating vertically through the shelf and reflecting from the ocean, and is found to be 576 ± 8 m. Introducing a baseline of 543 ± 7m between radio transmitter and receiver allowed the computation of the basal reflection coefficient, R, separately from englacial loss. The depth-averaged attenuation length of the ice column, 〈L〉 is shown to depend linearly on frequency. The best fit (95% confidence level) is 〈L(ν)〉= (460±20) − (180±40)ν m (20 dB km−1), for the frequencies ν = [0.100–0.850] GHz, assuming no reflection loss. The mean electric-field reflection coefficient is (1.7 dB reflection loss) across [0.100–0.850] GHz, and is used to correct the attenuation length. Finally, the reflected power rotated into the orthogonal antenna polarization i

Livetime and sensitivity of the ARIANNA Hexagonal Radio Array

The ARIANNA collaboration completed the installation of the hexagonal radio array (HRA) in December 2014, serving as a pilot program for a planned high energy neutrino telescope located about 110 km south of McMurdo Station on the Ross Ice Shelf near the coast of Antarctica. The goal of ARIANNA is to measure both diffuse and point fluxes of astrophysical neutrinos at energies in excess of 1016 eV. Upgraded hardware has been installed during the 2014 deployment season and stations show a livetime of better than 90% between commissioning and austral sunset. Though designed to observe radio pulses from neutrino interactions originating within the ice below each detector, one station was modified to study the low-frequency environment and signals from above. We provide evidence that the HRA observed both continuous emission from the Galaxy and a transient solar burst. Preliminary work on modeling the (weak) Galactic signal confirm the absolute sensitivity of the HRA detector system.Comment: Proceedings from the 34th ICRC2015, http://icrc2015.nl/, 8 pages, 6 figure