Arabidopsis CaM Binding Protein CBP60g Contributes to MAMP-Induced SA Accumulation and Is Involved in Disease Resistance against Pseudomonas syringae

Salicylic acid (SA)-induced defense responses are important factors during effector triggered immunity and microbe-associated molecular pattern (MAMP)-induced immunity in plants. This article presents evidence that a member of the Arabidopsis CBP60 gene family, CBP60g, contributes to MAMP-triggered SA accumulation. CBP60g is inducible by both pathogen and MAMP treatments. Pseudomonas syringae growth is enhanced in cbp60g mutants. Expression profiles of a cbp60g mutant after MAMP treatment are similar to those of sid2 and pad4, suggesting a defect in SA signaling. Accordingly, cbp60g mutants accumulate less SA when treated with the MAMP flg22 or a P. syringae hrcC strain that activates MAMP signaling. MAMP-induced production of reactive oxygen species and callose deposition are unaffected in cbp60g mutants. CBP60g is a calmodulin-binding protein with a calmodulin-binding domain located near the N-terminus. Calmodulin binding is dependent on Ca2+. Mutations in CBP60g that abolish calmodulin binding prevent complementation of the SA production and bacterial growth defects of cbp60g mutants, indicating that calmodulin binding is essential for the function of CBP60g in defense signaling. These studies show that CBP60g constitutes a Ca2+ link between MAMP recognition and SA accumulation that is important for resistance to P. syringae

ATL9, a RING Zinc Finger Protein with E3 Ubiquitin Ligase Activity Implicated in Chitin- and NADPH Oxidase-Mediated Defense Responses

Pathogen associated molecular patterns (PAMPs) are signals detected by plants that activate basal defenses. One of these PAMPs is chitin, a carbohydrate present in the cell walls of fungi and in insect exoskeletons. Previous work has shown that chitin treatment of Arabidopsis thaliana induced defense-related genes in the absence of a pathogen and that the response was independent of the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) signaling pathways. One of these genes is ATL9 ( = ATL2G), which encodes a RING zinc-finger like protein. In the current work we demonstrate that ATL9 has E3 ubiquitin ligase activity and is localized to the endoplasmic reticulum. The expression pattern of ATL9 is positively correlated with basal defense responses against Golovinomyces cichoracearum, a biotrophic fungal pathogen. The basal levels of expression and the induction of ATL9 by chitin, in wild type plants, depends on the activity of NADPH oxidases suggesting that chitin-mediated defense response is NADPH oxidase dependent. Although ATL9 expression is not induced by treatment with known defense hormones (SA, JA or ET), full expression in response to chitin is compromised slightly in mutants where ET- or SA-dependent signaling is suppressed. Microarray analysis of the atl9 mutant revealed candidate genes that appear to act downstream of ATL9 in chitin-mediated defenses. These results hint at the complexity of chitin-mediated signaling and the potential interplay between elicitor-mediated signaling, signaling via known defense pathways and the oxidative burst

Travel bans and scientific mobility: utility of asymmetry and affinity indexes to inform science policy

This study explores the international profiles in collaboration and mobility of countries included in the so-called “travel bans” implemented by US President Trump as executive order in 2017. The objective of this research is to analyze the exchange of knowledge between countries and the relative importance of specific countries in order to inform evidence-based science policy. The work serves as a proof-of-concept of the utility of asymmetry and affinity indexes for collaboration and mobility. Comparative analyses of these indicators can be useful for informing immigration policies and motivating collaboration and mobility relationships—emphasizing the importance of geographic and cultural similarities. Egocentric and relational perspectives are analyzed to provide various lenses on the importance of countries. Our analysis suggests that comparisons of collaboration and mobility from an affinity perspective can identify discrepancies between levels of collaboration and mobility. This approach can inform international immigration policies and, if extended, demonstrate potential partnerships at several levels of analysis (e.g., institutional, sectoral, state/province).Merit, Expertise and Measuremen

Predatory publishing in Scopus: evidence on cross-country differences lacks justification

Merit, Expertise and Measuremen

Predatory publishing in Scopus: evidence on cross-country differences lacks justification

Merit, Expertise and Measuremen

On the Mesoscale Structure of Coronal Mass Ejections at Mercury’s Orbit: BepiColombo and Parker Solar Probe Observations

On 2022 February 15, an impressive filament eruption was observed off the solar eastern limb from three remote-sensing viewpoints, namely, Earth, STEREO-A, and Solar Orbiter. In addition to representing the most-distant observed filament at extreme ultraviolet wavelengths—captured by Solar Orbiter's field of view extending to above 6 R _⊙ —this event was also associated with the release of a fast (∼2200 km s ^−1 ) coronal mass ejection (CME) that was directed toward BepiColombo and Parker Solar Probe. These two probes were separated by 2° in latitude, 4° in longitude, and 0.03 au in radial distance around the time of the CME-driven shock arrival in situ. The relative proximity of the two probes to each other and the Sun (∼0.35 au) allows us to study the mesoscale structure of CMEs at Mercury's orbit for the first time. We analyze similarities and differences in the main CME-related structures measured at the two locations, namely, the interplanetary shock, the sheath region, and the magnetic ejecta. We find that, despite the separation between the two spacecraft being well within the typical uncertainties associated with determination of CME geometric parameters from remote-sensing observations, the two sets of in situ measurements display some profound differences that make understanding the overall 3D CME structure particularly challenging. Finally, we discuss our findings within the context of space weather at Mercury's distance and in terms of the need to investigate solar transients via spacecraft constellations with small separations, which has been gaining significant attention during recent years