DataSheet1_Wound healing mechanism of antimicrobial peptide cathelicidin-DM.pdf

Background and Purpose: Chronic wound infections and the development of antibiotic resistance are serious clinical problems that affect millions of people worldwide. Cathelicidin-DM, an antimicrobial peptide from Duttaphrynus melanostictus, has powerful antimicrobial activity and wound healing efficacy. So, it could be a potential candidate to address this problem. In this paper, we investigate the wound healing mechanism of cathelicidin-DM to establish a basis for preclinical studies of the drug.Experimental Approach: The effects of cathelicidin-DM on cell proliferation and migration, cytokines, and mitogen-activated protein kinase (MAPK) signaling pathways were examined. Then mice whole skin wound model was constructed to evaluate the wound healing activity of cathelicidin-DM, and further histological changes in the wounds were assessed by hematoxylin-eosin staining (H&E) and immunohistochemical assays.Key Results: Cathelicidin-DM promotes the proliferation of HaCaT, HSF, and HUVEC cells in a concentration-dependent manner and the migration of HSF, HUVEC, and RAW.264.7 cells. Moreover,cathelicidin-DM can involve in wound healing through activation of the MAPK signaling pathway by upregulating phosphorylation of ERK, JNK, and P38. However, cathelicidin-DM didn’t affect the secretion of IL-6 and TNF-α. At the animal level, cathelicidin-DM accelerated skin wound healing and early debridement in mice as well as promoted re-epithelialization and granulation tissue formation, α-SMA expression, and collagen I deposition in mice.Conclusion and Implications: Our data suggest that cathelicidin-DM can be engaged in the healing of infected and non-infected wounds through multiple pathways, providing a new strategy for the treatment of infected chronic wounds.</p

Structure, Magnetism, and Tunable Negative Thermal Expansion in (Hf,Nb)Fe₂ Alloys

Structure, Magnetism, and Tunable Negative Thermal Expansion in (Hf,Nb)Fe₂ Alloy

Zero Thermal Expansion in Magnetic and Metallic Tb(Co,Fe)₂ Intermetallic Compounds

Due to the advantage of invariable length with temperatures, zero thermal expansion (ZTE) materials are intriguing but very rare especially for the metals based compounds. Here, we report a ZTE in the magnetic intermetallic compounds of Tb­(Co,Fe)₂ over a wide temperature range (123–307 K). A negligible coefficient of thermal expansion (α_l = 0.48 × 10^–6 K^–1) has been found in Tb­(Co_1.9Fe_0.1). Tb­(Co,Fe)₂ exhibits ferrimagnetic structure, in which the moments of Tb and Co/Fe are antiparallel alignment along the <i>c</i> axis. The intriguing ZTE property of Tb­(Co,Fe)₂ is formed due to the balance between the negative contribution from the Tb magnetic moment induced spontaneous magnetostriction and the positive role from the normal lattice expansion. The present ZTE intermetallic compounds are also featured by the advantages of wide temperature range, high electrical conductivity, and relatively high thermal conductivity

Zero Thermal Expansion in Magnetic and Metallic Tb(Co,Fe)₂ Intermetallic Compounds

Due to the advantage of invariable length with temperatures, zero thermal expansion (ZTE) materials are intriguing but very rare especially for the metals based compounds. Here, we report a ZTE in the magnetic intermetallic compounds of Tb­(Co,Fe)₂ over a wide temperature range (123–307 K). A negligible coefficient of thermal expansion (α_l = 0.48 × 10^–6 K^–1) has been found in Tb­(Co_1.9Fe_0.1). Tb­(Co,Fe)₂ exhibits ferrimagnetic structure, in which the moments of Tb and Co/Fe are antiparallel alignment along the <i>c</i> axis. The intriguing ZTE property of Tb­(Co,Fe)₂ is formed due to the balance between the negative contribution from the Tb magnetic moment induced spontaneous magnetostriction and the positive role from the normal lattice expansion. The present ZTE intermetallic compounds are also featured by the advantages of wide temperature range, high electrical conductivity, and relatively high thermal conductivity