Mir-382 Promotes Differentiation of Rat Liver Progenitor Cell WB-F344 by Targeting Ezh2

Background/Aims: Liver progenitor cells (LPCs) were considered as a promising hepatocyte source of cell therapy for liver disease due to their self-renewal and differentiation capacities, while little is known about the mechanism of LPC differentiate into hepatocytes. This study aims to explore the effect of miR-382, a member of Dlk1-Dio3 microRNA cluster, during hepatic differentiation from LPCs. Methods: In this study, we used rat liver progenitor cell WB-F344 as LPC cell model and HGF as inducer to simulate the process of LPCs hepatic differentiation, then microRNAs were quantified by qPCR. Next, WB-F344 cell was transfected with miR-382 mimics, then hepatocyte cell trait was characterized by multiple experiments, including that periodic acid schiff staining and cellular uptake and excretion of indocyanine green to evaluate the hepatocellular function, qPCR and Western Blotting analysis to detect the hepatocyte-specific markers (ALB, Ttr, Apo E and AFP) and transmission electron microscopy to observe the hepatocellular morphology. Moreover, Luciferase reporter assay was used to determine whether Ezh2 is the direct target of miR-382. Results: We found that miR-382 increased gradually and was inversely correlated with the potential target, Ezh2, during WB-F344 hepatic differentiation. In addition, functional studies indicated that miR-382 increased the level of hepatocyte-specific genes. Conclusions: This study demonstrates that miR-382 may be a novel regulator of LPCs differentiation by targeting Ezh2

Hirschmanniella shamimi Ahmad 1972

Hirschmanniella shamimi Ahmad, 1972, female intersexes (Figs. 1, 2) Type material and locality: Slide HSI 1 and HSI 2 deposited in Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, China. Sample was collected in May 2006 from soil samples around rice roots, in Guangzhou, Guangdong Province, China. Measurements. See Table 1. Gubernaculum μm 7.6 7.1 – 8.2±0.2 – – (7.6–9.0) (7–9) Description. Body slightly curved ventrally when heat relaxed. Lip region rounded, with 5 annuli. Stylet knobs rounded. Pharyngeal gland lobes long, ventrally overlapping intestine, 249 μm and 252 μm from anterior end for HSI 1 and HSI 2 respectively. Excretory pore posterior to level of esophago-intestinal valve. Lateral fields with four lines, not areolated near mid-body. Tail elongate conoid, terminus with a fine mucro and a ventral notch. The female reproductive system developed. Vulva well developed, in shape similar with the normal female vulva. Gonads didelphic, with outstretched ovaries, anterior genital branch 353 μm and 267 μm long for HSI 1 and HSI 2 respectively, posterior genital branch 383 μm and 305 μm long for HS 1 and HSI 2 respectively. Spermatheca oval and filled with sperm cells. The male reproductive system reduced. Testis not detected. Spicules arcuate, well developed, similar to normal male. Gubernaculum simple, thin, slightly arcuate. Bursa extending 42.8 μm along ventral body in HSI 2, but absent in HSI 1.Published as part of Zhuo, Kan, Liao, Jinling, Cui, Ruqiang & Li, Yuzhong, 2009, First record of female intersex in Hirschmanniella shamimi Ahmad, 1972 (Nematoda: Pratylenchidae), with a checklist of intersexes in plant nematodes, pp. 61-68 in Zootaxa 1973 on page 62, DOI: 10.5281/zenodo.18509

Aphelenchoididae (Skarbilovich, 1947) Paramonov 1953

Family: Aphelenchoididae (Skarbilovich, 1947) Paramonov, 1953 Aphelenchoides brassicae Edward & Misra, 1969 (F) (Edward 1969) Aphelenchoides composticola Franklin, 1957 (F) (Anderson and Kimpinski 1977) Aphelenchoides parietinus (Bastian, 1865) Steiner, 1932 (F) (Krall 1959) Aphelenchoides saprophilus Franklin, 1957 (F) (Braasch 1987) Aphelenchoides sp. (F) (Slepetiene 1962) Aphelenchoides sp. (F) (Khera and Chaturvedi 1971) Order: Tylenchida Thorne, 1949 Family: Anguinidae Nicoll, 1935 (1926) Ditylenchus triformis Hirschmann & Sasser 1955, (F) (Hirschmann and Sasser 1955) Ditylenchus triformis Hirschmann & Sasser 1955, (F) (Liu and Liu 2002) Family: Belonolaimidae Whitehead, 1960 Tylenchorhynchus capitatus Allen, 1955 (F) (Wouts 1966) Tylenchorhynchus nilgiriensis Seshadri, Muthukrishnan & Shunmugam, 1967 (F) (Seshadri et al. 1967) Tylenchorhynchus sp. (F) (Dalmasso 1966) = Telotylenchus sp. Family: Heteroderidae Filipjev & Schuurmans Stekhoven, 1941 Heterodera trifolii Goffart, 1932 (M) (Wouts 1978) Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 (F & M) (Ishibashi 1965) Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 (M) (Varma et al. 1971) Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 (M) (Rajan et al. 1987) Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (M) (Chitwood 1949) Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (M) (Triantaphyllou 1960) Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (F) (Davide and Triantaphyllou 1968) Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (M) (Yu and Chen 1998) Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (M) (He and Pan 2000) Meloidogyne arenaria (Neal, 1889) Chitwood, 1949 (M) (McLeod and Khair 1973) = M. thamesi Family: Hoplolaimidae Filipjev, 1949 Helicotylenchus indicus Siddiqi, 1963 (F) (Renubala et al. 1992) Family: Pratylenchidae Thorne, 1949 Hirschmanniella abnormalis Renubala, Dhanachand & Gambhir, 1992 (F) (Renubala et al. 1992) Hirschmanniella oryzae (Soltwedel, 1889) Luc & Goodey, 1963 (M) (Kanwar et al. 1992) Hirschmanniella shamimi Ahmad, 1972 (F) (Present work) Class: Adenophorea von Linstow, 1905 Order: Dorylaimida Chitwood & Chitwood, 1933 Family: Longidoridae Thorne, 1935 Longidorus africanus Merny, 1966 (F) (Cohn and Mordechai 1968) Longidorus distinctus Lamberti, Choleva & Agostinelli, 1983 (F) (Krnjaic et al. 1999) Longidorus distinctus Lamberti, Choleva & Agostinelli, 1983 (F) (Liškova 2007) Longidorus elongates (De Man, 1876) Thorne & Swanger, 1936 (F) (Raschké and Boag 1981) Longidorus elongates (De Man, 1876) Thorne & Swanger, 1936 (F) (Robbins 1986) Longidorus helveticus Lamberti, Kunz, Grunder, Molinari, De Luca, Agostinelli & Radicci, 2001 (F) (Barsi and De Luca 2005) Longidorus macrosoma Hooper, 1961 (F) (Aboul-Eid and Coomans 1966) Xiphinema attorodorum Luc, 1961 (F) (Luc 1961) Xiphinema ingens Luc & Dalmasso, 1964 (F) (Lamberti et al. 1975) Xiphinema insigne Loos, 1949 (M) (Bajaj and Jairajpuri 1977)Published as part of Zhuo, Kan, Liao, Jinling, Cui, Ruqiang & Li, Yuzhong, 2009, First record of female intersex in Hirschmanniella shamimi Ahmad, 1972 (Nematoda: Pratylenchidae), with a checklist of intersexes in plant nematodes, pp. 61-68 in Zootaxa 1973 on pages 64-65, DOI: 10.5281/zenodo.18509

FIGURE 2. Hirschmanniella shamimi intersex. a in First record of female intersex in Hirschmanniella shamimi Ahmad, 1972 (Nematoda: Pratylenchidae), with a checklist of intersexes in plant nematodes

FIGURE 2. Hirschmanniella shamimi intersex. a, anterior region (HSI1); b, posterior half with vulva and spicule (HSI1), vulva and spicule showed by an arrow respectively; c, anterior genital branch of gonads (HSI1); d, tail (HSI1), showing spicule without bursa; e, anterior genital branch of gonads (HSI2); f, tail (HSI2), showing spicule and bursa

The sucker-like end-to-side arterial anastomosis for free flap in extremities reconstruction: a retrospective study of 78 cases

Abstract Background The application of end-to-side (ETS) anastomosis for flap transfer poses challenges, particularly in cases of significant size discrepancy between the donor and flap arteries. Herein, a novel ETS anastomosis technique, termed “sucker-like ETS anastomosis”, is developed to mitigate and rectify such vessel discrepancies. This study aims to evaluate the efficacy of this technique in tissue defect reconstruction through free flap transfer. Methods Between September 2018 and March 2023, the medical records and follow-up data of 78 patients who underwent free flap transfer using sucker-like ETS anastomosis for significant artery size discrepancies were collected and retrospectively analyzed. Results Among the 78 cases that received free flap transfer, the range of artery size discrepancy (flap artery vs donor artery) was 1:1.6–1:4 (mean: 1:2.5). Following anastomosis with the sucker-like ETS technique, 75 cases achieved flap survival without requiring additional surgical intervention, yielding a one-stage success rate of 96.2%. Three cases experienced post-operative venous crises, with two cases surviving after vein exploration and one case undergoing flap necrosis, necessitating a secondary skin graft. Seven cases faced delayed wound healing but eventually achieved complete healing following dressing changes. No arterial crisis was observed during hospitalization. With an average follow-up of 13 months, the surviving flaps exhibited excellent vitality without flap necrosis or pigment deposition. Overall, the application of sucker-like ETS arterial anastomosis for flap transfer resulted in a high overall surgical success rate of 98.7% (77/78). Conclusion The application of sucker-like ETS anastomosis for free flap transfer is highly effective, particularly in cases with significant size discrepancy between the recipient and donor arteries

The Effects of Electrolytic Multivitamins and Neomycin on Antioxidant Capacity and Intestinal Damage in Transported Lambs

Transport stress can cause damage to animals. In this experiment, 60 four-month-old lambs were randomly divided into three groups: CG (basal diet), EG (basal diet + 375 mg/d/lamb electrolytic multivitamin), and NG (basal diet + 200 mg/d/lamb neomycin). The results were as follows: during road transport, in all groups, the levels of SOD, T-AOC, and GSP-Px, and mRNA expressions of CAT, SOD, Nrf2, HO-1, and Bcl-2 in the jejunum and colon decreased (p p p p p p p p p p p p p p p < 0.01) decreased. In summary, road transport can cause a decrease in antioxidant activity and immunity of lambs and an increase in oxidative damage. Electrolytic multivitamins and neomycin can improve immune function and potentially reduce oxidative damage to the jejunum and colon

An Ultra-Throughput Boost Method for Gamma-Ray Spectrometers

(1) Background: Generally, in nuclear medicine and nuclear power plants, energy spectrum measurements and radioactive nuclide identification are required for evaluation of strong radiation fields to ensure nuclear safety and security; thereby, damage is prevented to nuclear facilities caused by natural disasters or the criminal smuggling of nuclear materials. High count rates can lead to signal accumulation, negatively affecting the performance of gamma spectrometers, and in severe cases, even damaging the detectors. Higher pulse throughput with better energy resolution is the ultimate goal of a gamma-ray spectrometer. Traditionally, pileup pulses, which cause dead time and affect throughput, are rejected to maintain good energy resolution. (2) Method: In this paper, an ultra-throughput boost (UTB) off-line processing method was used to improve the throughput and reduce the pileup effect of the spectrometer. Firstly, by fitting the impulse signal of the detector, the response matrix was built by the functional model of a dual exponential tail convolved with the Gaussian kernel; then, a quadratic programming method based on a non-negative least squares (NNLS) algorithm was adopted to solve the constrained optimization problem for the inversion. (3) Results: Both the simulated and experimental results of the UTB method show that most of the impulses in the pulse sequence from the scintillator detector were restored to δ-like pulses, and the throughput of the UTB method for the NaI(Tl) spectrometer reached 207 kcps with a resolution of 7.71% @661.7 keV. A reduction was also seen in the high energy pileup phenomenon. (4) Conclusions: We conclude that the UTB method can restore individual and piled-up pulses to δ-like sequences, effectively boosting pulse throughput and suppressing high-energy tailing and sum peaks caused by the pileup effect at the cost of a slight loss in energy resolution