Condiciones Laborales y de Salud de Los Trabajadores de la Maquila del Tabaco. Ciudad El Paraíso, Honduras. Octubre 2003 a Marzo 2004.

El propósito es describir las características socio demográficas, las condiciones de trabajo y situación de salud de los trabajadores para aportar información y conocer la magnitud y trascendencia de esta problemática y tomar decisiones necesarias de prevención

Potential-Modulated Electrochemiluminescence of Carbon Nitride Nanosheets for Dual-Signal Sensing of Metal Ions

As an emerging semiconductor, graphite-phase polymeric carbon nitride (GPPCN) has drawn much attention not only in photocatalysis but also in optical sensors such as electrochemiluminescence (ECL) sensing of metal ions. However, when the concentrations of interfering metal ions are several times higher than that of the target metal ion, it is almost impossible to distinguish which metal ion changes the ECL signals in real sample detection. Herein, we report that the dual-ECL signals could be actuated by different ECL reactions merely from GPPCN nanosheets at anodic and cathodic potentials, respectively. Interestingly, the different metal ions exhibited distinct quenching/enhancement of the ECL signal at different driven potentials, presumably ascribed to the diversity of energy-level matches between the metal ions and GPPCN nanosheets and catalytic interactions of the intermediate species in ECL reactions. On this basis, without any labeling and masking reagents, the accuracy and reliability of sensors based on the ECL of GPPCN nanosheets toward metal ions were largely improved; thus, the false-positive result caused by interferential metal ions could be effectively avoided. As an example, the proposed GPPCN ECL sensor with a detection limit of 1.13 nM was successfully applied for the detection of trace Ni²⁺ ion in tap and lake water

Reversible Assembly of Graphitic Carbon Nitride 3D Network for Highly Selective Dyes Absorption and Regeneration

Responsive assembly of 2D materials is of great interest for a range of applications. In this work, interfacial functionalized carbon nitride (CN) nanofibers were synthesized by hydrolyzing bulk CN in sodium hydroxide solution. The reversible assemble and disassemble behavior of the as-prepared CN nanofibers was investigated by using CO₂ as a trigger to form a hydrogel network at first. Compared to the most widespread absorbent materials such as active carbon, graphene and previously reported supramolecular gel, the proposed CN hydrogel not only exhibited a competitive absorbing capacity (maximum absorbing capacity of methylene blue up to 402 mg/g) but also overcame the typical deficiencies such as poor selectivity and high energy-consuming regeneration. This work would provide a strategy to construct a 3D CN network and open an avenue for developing smart assembly for potential applications ranging from environment to selective extraction

Metal-Free All-Carbon Nanohybrid for Ultrasensitive Photoelectrochemical Immunosensing of alpha-Fetoprotein

C₆₀ can accept up to six electrons reversibly and show exceptional light absorption over the entire UV–vis spectrum, making it a potential photoactive probe for photoelectrochemical (PEC) bioassay. However, few successful works have been reported to apply fullerenes in PEC biosensing, partially because of the low electronic conductivity and poor interfacial interactions with targeted biomolecules. Herein, we report the addressing of these two obstacles by coupling high conductive graphite flake (Gr), graphene oxide (GO) with sufficient oxygen-containing functional groups, and an alkylated C₆₀ (AC₆₀) into a metal-free all-carbon nanohybrid (AC₆₀-Gr-GO) via harnessing delicate noncovalent interactions among them through a facile mechanical grinding. It was revealed that the as-obtained AC₆₀-Gr-GO nanohybrid not only showed conspicuous enhancement of photocurrent up to 35 times but also offered rich anchors for bioconjugation. With detection of alpha-fetoprotein as an example, the AC₆₀-Gr-GO based PEC immunosensor demonstrated a broad linear detection range (1 pg·mL^–1 to 100 ng·mL^–1) and a detection limit as low as 0.54 pg·mL^–1, superior/competitive to PEC immunosensors for AFP in previous reports. By a proper reinforcement in conductivity and biointerface engineering, this work may provide a new way to use fullerenes as photoactive materials in more general PEC biosensing

Chemically Modulated Carbon Nitride Nanosheets for Highly Selective Electrochemiluminescent Detection of Multiple Metal-ions

Chemical structures of two-dimensional (2D) nanosheet can effectively control the properties thus guiding their applications. Herein, we demonstrate that carbon nitride nanosheets (CNNS) with tunable chemical structures can be obtained by exfoliating facile accessible bulk carbon nitride (CN) of different polymerization degree. Interestingly, the electrochemiluminescence (ECL) properties of as-prepared CNNS were significantly modulated. As a result, unusual changes for different CNNS in quenching of ECL because of inner filter effect/electron transfer and enhancement of ECL owing to catalytic effect were observed by adding different metal ions. On the basis of this, by using various CNNS, highly selective ECL sensors for rapid detecting multiple metal-ions such as Cu²⁺, Ni²⁺, and Cd²⁺ were successfully developed without any labeling and masking reagents. Multiple competitive mechanisms were further revealed to account for such enhanced selectivity in the proposed ECL sensors. The strategy of preparing CNNS with tunable chemical structures that facilely modulated the optical properties would open a vista to explore 2D carbon-rich materials for developing a wide range of applications such as sensors with enhanced performances

Vx3-Functionalized Alumina Nanoparticles Assisted Enrichment of Ubiquitinated Proteins from Cancer Cells for Enhanced Cancer Immunotherapy

A simple and effective strategy was developed to enrich ubiquitinated proteins (UPs) from cancer cell lysate using the α-Al₂O₃ nanoparticles covalently linked with ubiquitin binding protein (Vx3) (denoted as α-Al₂O₃–Vx3) via a chemical linker. The functionalized α-Al₂O₃–Vx3 showed long-term stability and high efficiency for the enrichment of UPs from cancer cell lysates. Flow cytometry analysis results indicated dendritic cells (DCs) could more effectively phagocytize the covalently linked α-Al₂O₃–Vx3-UPs than the physical mixture of α-Al₂O₃ and Vx3-UPs (α-Al₂O₃/Vx3-UPs). Laser confocal microscopy images revealed that α-Al₂O₃–Vx3-UPs localized within the autophagosome of DCs, which then cross-presented α-Al₂O₃–Vx3-UPs to CD8⁺ T cells in an autophagosome-related cross-presentation pathway. Furthermore, α-Al₂O₃–Vx3-UPs enhanced more potent antitumor immune response and antitumor efficacy than α-Al₂O₃/cell lysate or α-Al₂O₃/Vx3-UPs. This work highlights the potential of using the Vx3 covalently linked α-Al₂O₃ as a simple and effective platform to enrich UPs from cancer cells for the development of highly efficient therapeutic cancer vaccines

Additional file 4:Figure S1. of Small RNA sequencing reveals a role for sugarcane miRNAs and their targets in response to Sporisorium scitamineum infection

Length distribution of the unique sRNA sequences in the four libraries. RCK and YACK: ROC22 and YA05-179 under sterile water stress after 48 h, respectively; RT and YAT: ROC22 and YA05-179 under Sporisorium scitamineum stress after 48 h, respectively. (TIF 35 kb