DNA Polymorphism of Insulin-like Growth Factor-binding Protein-3 Gene and Its Association with Cashmere Traits in Cashmere Goats

Insulin-like growth factor binding protein-3 (IGFBP-3) gene is important for regulation of growth and development in mammals. The present investigation was carried out to study DNA polymorphism by PCR-RFLP of IGFBP-3 gene and its effect on fibre traits of Chinese Inner Mongolian cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length. Four hundred and forty-four animals were used to detect polymorphisms in the hircine IGFBP-3 gene. A 316-bp fragment of the IGFBP-3 gene in exon 2 was amplified and digested with HaeIII restriction enzyme. Three patterns of restriction fragments were observed in the populations. The frequency of AA, AB and BB genotypes was 0.58, 0.33 and 0.09 respectively. The allelic frequency of the A and B allele was 0.75 and 0.25 respectively. Nucleotide sequencing revealed a C>G transition in the exon 2 region of the IGFBP-3 gene resulting in R158G change which caused the polymorphism. Least squares analysis revealed a significant effect of genotypes on cashmere weight (p0.05). The animals of AB and BB genotypes showed higher cashmere weight, cashmere fibre length and hair length than the animals possessing AA genotype. These results suggested that polymorphisms in the hircine IGFBP-3 gene might be a potential molecular marker for cashmere weight in cashmere goats

The effects of the method and dose of cyclophosphamide administration on cashmere shedding

The objective of this study was to investigate the effects of the method and dose of cyclophosphamide (CPA) administration on cashmere shedding. Thirtytwo castrated Liaoning cashmere goats were randomly allotted to four groups, with eight replicates in each group. Goats in the four groups were injected intravenously or intramuscularly with CPA at doses of 20 or 25 mg kg−1 body weight (BW), respectively. Feed intake and BW were recorded, and erythrocyte count, hemoglobin content, and cashmere weight were determined. It was found that the CPA administration method had no significant effect on feed intake or BW of cashmere goats. Cyclophosphamide injection can significantly decrease the erythrocyte count and hemoglobin content of cashmere goats, but the effects are dependent on injection method and CPA dose. The injection method and dose did not significantly influence cashmere weight, but the method had significant effects on time to initiate shedding and regrown hair length. Regrown hair was longest by intramuscular injection with 20 mg kg−1 BW, which also caused the least erythema on the epidermis during the days after shedding. The results indicate that the CPA administration method can significantly influence cashmere shedding. Intramuscular injection of CPA at a dose of 20 mg kg−1 BW was found to be relatively beneficial for hair removal and regrowth in cashmere goat

Promotion of cashmere growth by sulfur supplements in cashmere goats

This study was conducted to investigate effects of inorganic and organic sulfur supplements on cashmere growth and their differences. Thirty-six six-month-old female Liaoning cashmere goats with a body weight of approximately 25 kg and good health were randomly assigned to three treatments: control, ZnSO4 and HMBi (2-hydroxy-4-(methylthio) butyric acid isopropyl ester). The three groups were fed a basal diet, a ZnSO4 diet (supplemented with 0.63% ZnSO4.H2O) and an HMBi diet (supplemented with 1.27% HMBi), respectively. Blood and cashmere samples were collected at the end of the three-month experimental period. The plasma concentrations of total protein, urea nitrogen, ammonia and amino acids; the cashmere content of amino acids and sulfur contents; the cashmere growth rates; and the diameter of the cashmere fibres were determined. The results indicated that dietary supplementation with ZnSO4 or HMBi can decrease the plasma urea nitrogen concentration and increase concentrations of total protein and methionine in plasma. In addition, the two types of sulfur supplements appeared to increase the methionine, cysteine and sulfur contents in cashmere fibres. Furthermore, the supplements can accelerate cashmere growth, with no significant effect on cashmere fineness. The promotion of cashmere growth probably stems from the improvement in the protein metabolic balance, sulfur retention and sulfur-containing amino acids synthesis in cashmere goats following the ZnSO4 or HMBi supplementation. In general, the ZnSO4 supplement promotes greater cashmere growth than the HMBi supplement under the experimental conditions

Evaluation and Spatial Characteristics of Cooperation among Tourist Attractions Based on a Geographic Information System: A Case Study of The Yangtze River Delta Region, China

With the development of global economic integration and the gradual formation of unified tourism markets, strengthening regional tourism cooperation has become an internal requirement of regional tourism development but also a new way of sustainably developing tourism. This study selected the key factors affecting the cooperation of tourist attractions, including the competitiveness of tourist attractions and the relationships among tourist attractions, and established an evaluation index system and mathematical model of tourist attractions’ cooperation. Furthermore, the level of cooperation was evaluated. According to the value of the cooperation level, the spatial characteristics of the cooperation level were analyzed using a geographic information systems analytical method, which can better reflect the competitiveness, relationships, and overall cooperation level of tourist attractions. The results showed that the tourism competitiveness of tourist attractions was generally strong and their internal relations relatively close, and the overall tourism cooperation level was high. However, a two-dimensional four-quadrant map revealed that there were still great differences in tourism competitiveness among tourist attractions and their internal relations. Twenty-three tourist attractions exhibited weak tourism competitiveness and sparse relationships with other tourist attractions. The tourism competitiveness of tourist attractions and their internal relations and tourism cooperation level showed positive spatial autocorrelation and spatial agglomeration characteristics. The spatial differentiation of an “inverted U-shape” indicated that the cooperation level, tourism competitiveness, and mutual relations of tourist attractions were not balanced and that a stable and gradual spatial transformation had not been achieved. This study can provide valuable insights for the government to formulate policies and measures for regional tourism cooperation, carry out regional joint marketing, and help tourism enterprises design tourist routes

Evaluation and Spatial Characteristics of Cooperation among Tourist Attractions Based on a Geographic Information System: A Case Study of The Yangtze River Delta Region, China

With the development of global economic integration and the gradual formation of unified tourism markets, strengthening regional tourism cooperation has become an internal requirement of regional tourism development but also a new way of sustainably developing tourism. This study selected the key factors affecting the cooperation of tourist attractions, including the competitiveness of tourist attractions and the relationships among tourist attractions, and established an evaluation index system and mathematical model of tourist attractions’ cooperation. Furthermore, the level of cooperation was evaluated. According to the value of the cooperation level, the spatial characteristics of the cooperation level were analyzed using a geographic information systems analytical method, which can better reflect the competitiveness, relationships, and overall cooperation level of tourist attractions. The results showed that the tourism competitiveness of tourist attractions was generally strong and their internal relations relatively close, and the overall tourism cooperation level was high. However, a two-dimensional four-quadrant map revealed that there were still great differences in tourism competitiveness among tourist attractions and their internal relations. Twenty-three tourist attractions exhibited weak tourism competitiveness and sparse relationships with other tourist attractions. The tourism competitiveness of tourist attractions and their internal relations and tourism cooperation level showed positive spatial autocorrelation and spatial agglomeration characteristics. The spatial differentiation of an “inverted U-shape” indicated that the cooperation level, tourism competitiveness, and mutual relations of tourist attractions were not balanced and that a stable and gradual spatial transformation had not been achieved. This study can provide valuable insights for the government to formulate policies and measures for regional tourism cooperation, carry out regional joint marketing, and help tourism enterprises design tourist routes

Effects of 2-hydroxy-4(methylthio) butanoic acid isopropyl ester on rumen fermentation in cashmere goats

The impact of dietary 2-hydroxy-4-(methylthio) butyric acid isopropyl ester (HMBi) on rumen fermentation in cashmere goats was investigated to determine the effect of this ester on cashmere production. Four healthy castrated Liaoning cashmere goats with permanent rumen cannulas were assayed for four periods. In each period, goats were fed a different diet: control diet (containing 0% MetaSmart (an HMBi product)), diet I (containing 0.85% MetaSmart), diet II (containing 1.27% MetaSmart) or diet III (containing 1.70% MetaSmart). Rumen pH, ammonia-N concentration, volatile fatty acid (VFA) concentrations and total protein concentration were determined after feeding in each period. Results showed that rumen pH significantly increased and rumen ammonia-N concentration significantly decreased when the diet was supplemented with 0.85%, 1.27% and 1.70% MetaSmart. Diets with 1.27% and 1.70% MetaSmart increased rumen total VFA concentration, and 3 hours post feeding all three HMBi diets increased acetic acid concentration. Rumen total protein concentration significantly increased in the goats fed 1.27% or 1.70% MetaSmart. Thus, diets supplemented with HMBi significantly affected rumen pH, ammonia-N concentration, VFA concentrations and total protein content. Dietary HMBi can promote rumen fermentation in cashmere goats

通过光学相干断层扫描血管成像检测发现袖状胃切除术改善肥胖队列的微血管表型

Abstract Aims To examine how metabolic status is associated with microvascular phenotype and to identify variables associated with vascular remodeling after bariatric surgery, using noninvasive optical coherence tomography angiography (OCTA). Methods The study included 136 obese subjects scheduled for bariatric surgery and 52 normal‐weight controls. Patients with obesity were divided into metabolically healthy obesity (MHO) and metabolic syndrome (MetS) groups according to the diagnosis criteria of the Chinese Diabetes Society. Retinal microvascular parameters were measured by OCTA, including superficial capillary plexus (SCP) and deep capillary plexus (DCP) vessel densities. Follow‐ups were performed at the baseline and 6 months after bariatric surgery. Results Fovea SCP, average DCP, fovea DCP, parafovea DCP, and perifovea DCP vessel densities were significantly lower in the MetS group, compared to controls (19.91% vs. 22.49%, 51.60% vs. 54.20%, 36.64% vs. 39.14%, 56.24% vs. 57.65% and 52.59% vs. 55.58%, respectively, all p < .05). Parafovea SCP, average DCP, parafovea DCP, and perifovea DCP vessel densities significantly improved in patients with obesity 6 months after surgery, compared to baseline (54.21% vs. 52.97%, 54.43% vs. 50.95%, 58.29% vs. 55.54% and 55.76% vs. 51.82%, respectively, all p < .05). Multivariable analyses showed that baseline blood pressure and insulin were independent predictors of vessel density changes 6 months after surgery. Conclusions Retinal microvascular impairment occurred mainly in MetS rather than MHO patients. Retinal microvascular phenotype improved 6 months after bariatric surgery and baseline blood pressure and insulin status may be key determinants. OCTA may be a reliable method to evaluate the microvascular complications associated with obesity