250 research outputs found
New digital infrastructure, cross-border e-commerce and global vision of creating Electronic World Trade Platform
The rapid development of cross-border e-commerce has integrated with the global economy more closely in the past decade. How to create global digital customs to facilitate cross-border e-commerce on the basis of national Single Window system has become an important task for national governments and international organizations such as World Customs Organizations? The paper aims to explore the relationship between technological progress, cross-border e-commerce and the establishment of global digital customs from the dimensions of the latest development of new digital infrastructure, national Single Window system and global vision of creating Electronic World Trade Platform (eWTP). It is argued that cross-border e-commerce platforms, national Single Window and eWTP, all of which are indispensable for the establishment of global digital customs, have close linkages in business regulation, data sharing and information exchange. The establishment of global digital customs requires global governance through the joint efforts by firms, national governments and international organizations
What Are the New Challenges of the Current Cancer Biomarkers?
Biomarkers are emerging research filed in the past decade. Even though numerous biomarkers were reported, the efficiency of cancer therapy remains low. So the question emerges as to how much can we trust the current biomarkers on cancer therapy? In this upcoming chapter, we would like to illustrate the outcomes of classical cancer therapies on advanced pancreatic cancer disclosed successful, neutral and failed in clinical trials. The analysis will be carried on the perspective interdisciplinary on the biomarkers including anatomy, physiology, pharmacology, biochemistry, history path and development of pharmacy. Particular in-depth insight may open a window for new researches and lighting the therapies
Virulent and attenuated strains of Trichoderma citrinoviride mediated resistance and biological control mechanism in tomato
IntroductionRoot-knot nematode disease is one of the world's most serious vegetable crop diseases. In recent years, Trichoderma spp. has been widely used in root-knot nematode disease control as a biological control agent.MethodsVirulent and attenuated strains of Trichoderma citrinoviride mediated resistance and biological control mechanism in tomato were determined.ResultsPreliminary experiments found differences in nematicidal virulence among Trichoderma citrinoviride. The 24-hour corrected mortality rate of the virulent strainT1910 was as high as 92.37%, with an LC50 of 0.5585 against the second juveniles (J2s) of Meloidogyne incognita. And the attenuated strain TC9 was 23.01%, the LC50 was 2.0615, so the virulent strain T1910 had a more substantial effect on the J2s than the attenuated strain. We found that the strong virulent strain T1910 have a good control effect on M. incognita by the pot experiment of tomato than that of the attenuated virulent strain TC9,especially the J2 and J4 numbers were inhibited inside the root knots of tomato. Theinhibition rates of virulent strains reached 85.22% and 76.91%, followed by attenuatedstrain TC9, which were 63.16% and 59.17%, respectively. To reveal the differences intomato defense pathways induced by different virulent strains, qRT-PCR was further usedto detect changes in the expression of inducement-related genes. The results showed thatthe TC9 was significantly upregulated at 5dpi, LOX1, PR1, and PDF1.2. The PR5 gene ofthe virulent strain T1910 was highly upregulated, and the JA pathway was activated laterbut weaker than the attenuated strain. The results of this study revealed that thebiocontrol mechanism of T. citrinoviride as poison killing through the virulent strain T1910 and induced resistance to M. incognita through attenuated strain, although virulence degradation also has an induced resistance effect. Moreover, the attenuated strain TC9 stimulated tomato immune response earlier than the virulent strain by nematode-associated molecular pattern-triggered (NAMP).DiscussionTherefore, the research elucidated the mechanism of multiple control of Trichoderma spp. against M. incognita
Influence of medium modifications (optimization) on high nematicidal activity of the fermentation broth of Clostridium beijerinckii
Introduction: The nematode species Meloidogyne incognita has been responsible for significant financial losses within the agricultural sector. Nematophagous bacteria, characterised by their extensive distribution and broad spectrum of hosts, exhibit remarkable efficacy as natural antagonists against nematodes. Sneb518 (Clostridium beijerinckii) fermentation broth displayed substantial biocontrol activity against M. incognita in previous research. Optimizing fermentation conditions is a fundamental technique for dramatically enhancing end product performance. There has been no such study conducted yet on enhancing the nematicidal activities of Sneb518 (Clostridium beijerinckii) fermentation using response surface methodology (RSM).Methods: The influence of strain Sneb518 fermentation media and conditions on nematicidal activity was examined using the three-factor technique and a Plackett-Burman design, and the interaction between various fermentation factors was examined using a Box-Behnken design. The present study employed response surface methodology (RSM) to examine and enhance the nematicidal activity of Sneb518 culture filtrates by identifying and optimising the influential components.Results: Glucose, peanut cake flour, and potassium chloride as carbon, nitrogen, and inorganic salts displayed considerably increased nematicidal potential in the present study. Furthermore, the corrected mortality of J2 ranged from 52.24% to 91.15% when utilizing the Box-Behnken design. These findings clearly support the application of RSM for medium optimization. Moreover, the outcomes of the validation experiment corresponded to the model predictions.Discussion: This research has enhanced the biocontrol ability of C. beijerinckii to control M. incognita and this research has led to the advancement of new biocontrol agents
Effects of season and diel cycle on hydroacoustic estimates of density, Target Strength, and vertical distribution of fish in Yudong Reservoir, a plateau deep water reservoir in southwest China in a plateau deep
Hydroacoustics is a non-invasive fish stock assessment sampling technique that plays an important role in fishery science and management. However, non-standard hydroacoustic surveys could lead to biased results, and the factor of the sampling period (e.g., season and diel cycle) is extremely critical as it can greatly affect hydroacoustic results. Efforts to improve the accuracy and credibility of the hydroacoustic survey results are getting more and more attention. Thus, we conducted two diel hydroacoustic surveys in situ in summer and winter to detect whether there were diel and seasonal differences in density, Target Strength (TS) and vertical distribution of fish. The results indicated that nighttime had significantly higher fish mean density than daytime in summer and winter. No significant difference between summer and winter daytime, however, significant difference between summer and winter nighttime, but this bias could be accepted from the fisheries management perspective; The mean TS of the summer daytime was significantly higher than that of summer nighttime, winter daytime and winter nighttime, but there were no significant differences among summer nighttime, winter daytime, and winter nighttime, mainly due to mean TS may be overestimated from fish schooling behavior during summer daytime; The fish vertical distribution had significant seasonal correlations and was more dispersed in different water layers during the nighttime, proving that the assessment was better at nighttime than during the daytime. Consequently, the hydroacoustic surveys in Yudong Reservoir and other similar plateau deep water reservoirs should be performed at nighttime, which will yield relatively accurate density and TS, and dispersed vertical distribution of fish
Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing
Description of a new record species of Heterodera from China (Tylenchida, Heteroderidae)
A new record species Heterodera ripae was found in the rhizosphere
soil of nettle (Urtica sp.) on the bank of the Mangniu River in
Shenyang City, Liaoning Province, China. The new record species can be
characterized by vulval cone bifenestrate with circular or subcircular
semifenestrae, vulval slit length averaging 34.2 μm, vulval bridge
mostly broad, underbridge weak, bullae absent;the second-stage
larvae stylet knobs rather wide and slightly projecting
anteriorly,tail length averaging 43.7 μm and hyaline region
length averaging 22.3 μm
LOCAL EXISTENCE OF CLASSICAL SOLUTIONS TO SHALLOW WATER EQUATIONS WITH CAUCHY DATA CONTAINING VACUUM
National Research Foundation of Korea (NRF) [2010-0029638]; Korea government (MEST); NSFC [10801111, 11171223]; Fundamental Research Funds for the Central Universities [2010121006]; NSF of Fujian Province of People's Republic of China [2010J05011]; NSF [DMS 0908207]In this paper, we investigate the Cauchy problem for the rotating shallow water equations with physical viscosity. We obtain the local existence of classical solutions without assuming the initial height is small or a small perturbation of some constant status. Moreover, the initial vacuum is allowed and the spatial measure of the set of vacuum can be arbitrarily large. In particular, the initial height can even have compact support; in this case, a blow-up example is given
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