Confirmation of myocardial-specific SOCS3 deletion in SOCS3-CKO mice.

<p>mRNA was prepared from the hearts and livers of wild-type (WT) mice 3 h after lipopolysaccharide (LPS) injection, and real-time PCR analyses for SOCS3 were performed. Values normalized to GAPDH are expressed (n = 5 per group). *<i>P</i> < 0.05 vs. WT pre-injection, §<i>P</i> < 0.05 vs. WT LPS injection.</p

Early and sustained expression of SOCS3 protein during myocardial IRI.

<p>(<b>A</b>) Total cell lysates (TCL) were prepared from the left ventricle of wild-type (WT) and SOCS3-CKO mice at the indicated times after reperfusion and were immunoprecipitated with anti-SOCS3 antibodies. The immunoprecipitates and total cell lysates were subjected to western blotting and probed with antibodies against SOCS3 and GAPDH, respectively. The graphs represent the time course expression of SOCS3 protein in WT mice after reperfusion (left) and the quantitative difference between WT and SOCS3-CKO mice 3 h after reperfusion (right; n = 5 per group). *<i>P</i> < 0.05 vs. WT pre-ischemia. §<i>P</i> < 0.05 vs. WT 3 h after reperfusion. AU indicates arbitrary units; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IRI, ischemia reperfusion injury. (<b>B</b>) Real-time PCR analysis for SOCS3 mRNA expression in mouse hearts pre-ischemia or 3 h after reperfusion (n = 5 for each group). *<i>P</i> < 0.05 vs. pre-ischemia.</p

Echocardiography measurements in wild-type (WT) and cardiac-specific SOCS3-CKO mice during the chronic phase.

<p>Echocardiography was performed at pre-ischemia and from day 1 to day 28 after reperfusion (n = 8 per group). Pooled data for echocardiographic measurements in WT and SOCS3-CKO mice. *<i>P</i> < 0.05 vs. WT mice. FS indicates fractional shortening; LVESD, left ventricular end systolic dimension; LVEDD, left ventricular end diastolic dimension; IVST, interventricular septum thickness; PWT, posterior left ventricular wall thickness.</p