A method for identification of the methylation level of CpG islands from NGS data

In the course of sample preparation for Next Generation Sequencing (NGS), DNA is fragmented by various methods. Fragmentation shows a persistent bias with regard to the cleavage rates of various dinucleotides. With the exception of CpG dinucleotides the previously described biases were consistent with results of the DNA cleavage in solution. Here we computed cleavage rates of all dinucleotides including the methylated CpG and unmethylated CpG dinucleotides using data of the Whole Genome Sequencing datasets of the 1000 Genomes project. We found that the cleavage rate of CpG is significantly higher for the methylated CpG dinucleotides. Using this information, we developed a classifier for distinguishing cancer and healthy tissues based on their CpG islands statuses of the fragmentation. A simple Support Vector Machine classifier based on this algorithm shows an accuracy of 84%. The proposed method allows the detection of epigenetic markers purely based on mechanochemical DNA fragmentation, which can be detected by a simple analysis of the NGS sequencing data

Conference Review. The First Siberian Legal Forum. ‘Specialization of Courts and Judges' (14-16 October 2014, Tyumen, Russia)

status: publishe

Particularities of the sustainable energy technologies and labour market in Russia

The energy sector is now experiencing a renaissance thanks to new opportunities offered by innovative technologies that are filling the market, for example in the field of storage and management of electrical energy. For the controlled development of distributed generation, maintaining the stability of the energy system while maintaining the price advantages of small generation, the speed of commissioning new power capacities and the scalability of the solutions used, a new subject of relations in the electricity market is proposed - an active energy complex. This is an energy system that is electrically connected within the general boundaries of the balance sheet membership, which includes energy receiving, generating, accumulating (if any), electric grid energy equipment and a control system for this energy system as a single complex in order to meet energy needs. There is a contradiction between the development of the labour market and the achievement of environmental well-being. On one hand, environmental pollution, depletion of natural capital has a negative impact on the state of the labour market, employment of the population, and on the other hand - activities to improve the environment, eliminate the accumulated environmental damage, the development of a market for environmental works, goods and services promote the expansion of employment, not only green, but general employment