“Revisiting the Past”: A Redescription of \u3ci\u3ePhysaloptera retusa\u3c/i\u3e (Nemata, Physalopteridae) from Material Deposited in Museums and New Material from Amazon Lizards = “Revisitando o passado”: Uma redescrição de \u3ci\u3ePhysaloptera retusa\u3c/i\u3e (Nemata, Physalopteridae) a partir de material depositado em museus e novo material de lagartos amazônicos

Abstract Physaloptera Rudolphi, 1819 is a genus of nematodes that includes approximately 100 species parasitic in vertebrates around the world. From these, approximately 30 occur in the Neotropical region, with nine reported from neotropical reptiles. Physaloptera spp. are recognized by their distinct morphology of the apical end and characters of the reproductive system. However, despite the fact that the morphological characters for species diagnosis have been firmly established, we frequently find identification problems regarding poorly detailed descriptions and poorly preserved specimens. These may lead to taxonomic incongruencies. Physaloptera retusa (Rudolphi, 1819) is the most common species of the genus and has been reported from several species of neotropical reptiles. Based on our reexaminations of nematode specimens identified as P. retusa from different museum collections, we provide a detailed redescription including the type material, voucher specimens and new specimens recovered currently and showed in this study with new morphological data obtained using light and scanning electron microscopy tools. Keywords: Helminths of reptiles, Amazon, taxonomical identification, morphology, scanning electron microscopy. Resumo Physaloptera Rudolphi, 1819 é um gênero de nematódeos que inclui aproximadamente 100 espécies parasitárias em vertebrados em todo o mundo. Destes, aproximadamente 30 ocorrem na região Neotropical, e nove foram reportados para répteis neotropicais. Physaloptera spp. são reconhecidas por sua morfologia distinta na extremidade apical e por caracteres do sistema reprodutivo, especialmente nos machos. No entanto, embora os caracteres morfológicos para o diagnóstico de espécies tenham sido estabelecidos, frequentemente são encontrados problemas de identificação em relação a descrições pouco detalhadas e espécimes mal preservados. Isto pode levar a incongruências taxonômicas e erros de identificação. Physaloptera retusa (Rudolphi, 1819) é a espécie mais comum do gênero e tem sido reportada para várias espécies de répteis neotropicais. Com base nos estudos das revisões de espécimes de nematódeos, identificados como P. retusa de diferentes coleções de museus, foi providenciada uma redescrição detalhada, incluindo-se o material-tipo, espécimes “voucher” e novos espécimes obtidos neste estudo com novos dados morfológicos, obtidos a partir de microscopia eletrônica de luz e varredura

A new species of Aplectana Railliet & Henry, 1916 (Nematoda: Cosmocercidae) in the Brazilian Amazon and the taxonomic status of Aplectana longa

Abstract Nematodes of the genus Aplectana Railliet & Henry, 1916 are common parasites of the digestive tract of amphibians and reptiles in the Neotropical region. During a parasite survey on Boana boans (Linnaeus, 1758), we found specimens of nematodes with Aplectana characteristics. We observed a set of characteristics that differs the species of our study from its congeners, and the present study describes a new species of Aplectana parasite of B. boans using light microscopy and scanning electron microscopy. Aplectana pella n. sp. has lateral alae and somatic papillae in males and females; males have equal short spicules, and the gubernaculum is absent. The arrangement of pairs of caudal papillae also differs from other species (2 precloacal,1 adcloacal, and 5 postcloacal+1 unpaired). In females, the vulva is simple, with non-prominent lips, and equatorial. This is the first record of the genus Aplectana parasitizing B. boans and the 58th species described for this genus. Additionally, we added the precloacal papillae pattern of A. delirae, and based on morphological and morphometric characteristics, we propose the reallocation of Aplectana longa to the genus Oxyascaris

Redescription of Oswaldocruzia chambrieri (Strongylida: Molineidae) from Rhinella margaritifera (Anura: Bufonidae) in Caxiuanã National Forest, Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-11-08T09:51:50Z No. of bitstreams: 1 arnaldo_maldonadojr_etal_IOC_2016.pdf: 5314000 bytes, checksum: 4e1df705c2bd8200866fed75654eefe0 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-11-08T10:01:06Z (GMT) No. of bitstreams: 1 arnaldo_maldonadojr_etal_IOC_2016.pdf: 5314000 bytes, checksum: 4e1df705c2bd8200866fed75654eefe0 (MD5)Made available in DSpace on 2018-11-08T10:01:06Z (GMT). No. of bitstreams: 1 arnaldo_maldonadojr_etal_IOC_2016.pdf: 5314000 bytes, checksum: 4e1df705c2bd8200866fed75654eefe0 (MD5) Previous issue date: 2016Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Biologia Celular e Helmintologia "Profa. Dra. Reinalda Marisa Lanfredi". Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Biologia Celular e Helmintologia "Profa. Dra. Reinalda Marisa Lanfredi". Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciiências Biológicas. Laboratório Multidisciplinar da Biologia a Distância. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Biologia Celular e Helmintologia "Profa. Dra. Reinalda Marisa Lanfredi". Belém, PA, Brasil.Abstract Oswaldocruzia chambrieri Ben Slimane et Durette-Desset, 1993 is redescribed from specimens collected from the small intestine of the South American common toad, Rhinella margaritifera, from Caxiuanã National Forest in Pará, Brazil, using light and scanning microscopy and molecular analysis of Cytochrome Oxidase I (COI) - coding regions of DNA. The discovered nematodes are characterized by a type III caudal bursa with two papillae, rays 4 with a median groove, and spicules divided into a blade, a shoe and a fork. Cervical alae are absent, the cephalic vesicle is divided into two portions, and the synlophe has low ridges without chitinous supports. The present study establishes the Caxiuanã National Forest as a new location for O. chambrieri, which had previously been reported as a parasite of R. margaritifera in Ecuador, uses light microscopy and scanning electron microscopy (SEM) to identify new morphological characters of the species and represents the second molecular sequence deposited for the Oswaldocruzia genus

New species of Serpinema (Nematoda: Camallanidae) from the scorpion mud turtle Kinosternon scorpioides (Testudines: Kinosternidae) from eastern Amazon, Brazil

The genus Serpinema Yeh, 1960 allocates parasitic nematodes of freshwater turtles distributed across the Oriental, Neotropical, and Palearctic regions. Five of 10 valid species of the genus Serpinema occur in the Neotropical region, and three have been recorded parasitizing Kinosternon scorpioides Linnaeus, 1766. In the present work, we describe a new species of Serpinema, a gastrointestinal parasite of K. scorpioides from the Brazilian Amazon, using light and scanning electron microscopy. Serpinema pelliculatus n. sp. differs from other species of the genus by the number and distribution of caudal papillae and spicule morphology. The new species is the seventh recorded for the Neotropical region